Method for extracting high-purity esculine and fraxin from Cortex Fraxini

Abstract

The invention provides a method for extracting high-purity esculine and fraxin from Cortex Fraxini, which comprises the following steps: pulverizing a Cortex Fraxini raw material and extracting under hot reflux by a conventional process, preparing a dual-water phase, dissolving, extracting, concentrating, desalinizing and crystallizing. The method mainly comprises the following steps: 1) preparing a dual-water phase system, and separating to obtain an upper phase and a lower phase for later use, and dissolving a Cortex Fraxini extract in the upper phase solvent; 2) centrifugating or filtering the solution containing the Cortex Fraxini extract, adding a right amount of lower phase solvent, sufficiently stirring and mixing, standing, separating the upper phase solution, and concentrating, thereby effectively removing inorganic substances, monosaccharides, polysaccharides. vegetable proteins, chlorophyl and other solid impurities in the Cortex Fraxini extract and enhancing the contents of esculine and fraxin; and removing salts and other impurities from the solution by conventional processes, and concentrating to a proper concentration, thereby sequentially obtaining the esculine and fraxin crystals. The method solves the problems of low crystallizing tendency, high production cost, complex operation and the like in the existing process of preparing esculine and fraxin from Cortex Fraxini, and has the characteristics of simple production equipment, environment friendliness and the like.

Description

technical field [0001] The invention relates to a method for dissolving the upper and lower phase solutions of the two-phase aqueous system formed by inorganic salts / water-miscible organic solvents and extracting auropeptin and auropeptin in the extract of fennel bark, belonging to the technical field of biochemical industry . Background technique [0002] At present, the main uses of high-purity alopecia are as follows: ① as a biochemical detection reagent: using the structural enzymes of some metabolically active bacteria to quickly hydrolyze alopecia to produce alopecia Ferric ammonium citrate or ferric citrate decomposed in the medium produced Fe 2+ reaction to generate blue-black substances, making the culture medium appear blue-black spots, which can visually and accurately identify certain types of oxygen-containing bacteria, and the test results can be obtained within 15-30 minutes at the fastest, which is different from traditional methods. Compared with the conve...

AI Technical Summary

Problems solved by technology

The main problem of this method is that the solution to be crystallized contains a large amount of plant polysaccharides, monosaccharides, proteins, etc., and has high viscosity and is not easy to crystallize.

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