Method for extracting high-purity esculine and fraxin from Cortex Fraxini
A kind of technology of aricin and aricin, which is applied in the fields of aricin and aricin, can solve the problems of difficult crystallization and high viscosity, and achieve the effects of low production cost, high product yield and easy control of production scale
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[0028] Below in conjunction with example the present invention is described in further detail. For the TLC and HPLC detection methods in the examples, refer to "Workbook of Chemical Reference Substances of Traditional Chinese Medicine" edited by Chen Dechang (China Medical Science and Technology Press, 2000, P129‐130).
[0029] Take 1 kg of Qinpi medicinal material (origin: Shaanxi), crush it into coarse powder, add 6 kg of ethanol, heat to reflux for 1 hour, and filter out the solution; then add 4 kg of ethanol and heat to reflux for 1 hour, filter out the solution, and combine the two extracts , concentrating under reduced pressure and recovering ethanol to obtain an extract with a specific gravity of 1.15 for subsequent use.
[0030] Keep the ambient temperature at 20°C, prepare a two-phase aqueous system with ammonium sulfate, ethanol, and water (the mass concentrations of ammonium sulfate and ethanol in the system are 14% and 30%, respectively), and after fully mixing, le...
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