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A method for producing Moringa polysaccharides by suspension culture of non-embryogenic cells of Moringa oleifera

A technology of cell suspension and Moringa polysaccharide, applied in the field of natural product biochemistry, to achieve the effects of shortened production cycle, stable output and high content of active ingredients

Active Publication Date: 2017-08-25
海南木辣达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no way to prepare Moringa polysaccharide based on plant cell suspension culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032](1) Take the immature seeds of Moringa oleifera as explants, cut the immature pods of Moringa oleifera into sections, sterilize them with 0.1% mercuric chloride for 10 minutes, rinse them with sterile water, break the pods with a sterile knife, and use Sterile tweezers take out the aseptic seeds, and further peel off the seed coat of the seeds simultaneously to obtain Moringa explants;

[0033] (2) Inoculate the Moringa oleifera explants prepared in step (1) into the callus induction medium, inoculate 2 Moringa oleifera explants in each bottle of culture medium, at a temperature of 27°C, with a light intensity of 3000Lux, and the daily light duration is Under the condition of 12 hours, non-embryogenic callus was obtained after 30 days of culture, and the average diameter of the non-embryogenic callus was 0.5 cm; the formula of the callus induction medium was: MS medium+brown sugar 30g / L+2, 4-D 3mg / L+NAA 0.5mg / L+0.8%g / L agar, pH 5.8;

[0034] (3) Transfer the non-embryog...

Embodiment 2

[0042] (1) Take the immature seeds of Moringa oleifera as explants, cut the immature pods of Moringa oleifera into sections, sterilize them with 0.1% mercuric chloride for 10 minutes, rinse them with sterile water, break the pods with a sterile knife, and use Sterile tweezers take out the aseptic seeds, and further peel off the seed coat of the seeds simultaneously to obtain Moringa explants;

[0043] (2) Inoculate the Moringa oleifera explant prepared in step (1) into the callus induction medium, inoculate 1 grain of Moringa oleifera explant in each bottle of culture medium, at a temperature of 24°C, with a light intensity of 4000 Lux, and the daily light duration is Under the condition of 8 hours, non-embryogenic callus was obtained after 25 days of culture, and the average diameter of the non-embryogenic callus was 0.6 cm; the formula of the callus induction medium was: MS medium+brown sugar 20g / L+2, 4-D 2mg / L+NAA 0.1mg / L+1%g / L agar, pH 5.5;

[0044] (3) Transfer the non-e...

Embodiment 3

[0052] (1) Take the immature seeds of Moringa oleifera as explants, cut the immature pods of Moringa oleifera into sections, sterilize them with 0.1% mercuric chloride for 10 minutes, rinse them with sterile water, break the pods with a sterile knife, and use Sterile tweezers take out the aseptic seeds, and further peel off the seed coat of the seeds simultaneously to obtain Moringa explants;

[0053] (2) Inoculate the Moringa oleifera explants prepared in step (1) into the callus induction medium, inoculate 1 to 2 grains of Moringa oleifera explants in each bottle of culture medium, at a temperature of 28°C, light intensity of 2000Lux, and daily light Under the condition of 10 hours, the non-embryogenic callus was obtained after 35 days of culture, and the average diameter of the non-embryogenic callus was 1 cm; the formula of the callus induction medium was: MS medium + brown sugar 40g / L + 2 , 4-D 1mg / L+NAA 1mg / L+1.2%g / L agar, pH 5.8;

[0054] (3) Transfer the non-embryogen...

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Abstract

The invention relates to the technical field of natural product biochemistry, and specifically discloses a method for producing moringa polysaccharides by suspension culture of non-embryogenic cells of moringa oleifera. The method adopts non-embryogenic cells of Moringa oleifera to produce Moringa polysaccharide, and the obtained non-embryogenic cells have a single type, fully dispersed cells, extremely high value-added efficiency, consistent growth cycle, and easy regulation of yield; induction and proliferation of Moringa oleifera explants After culturing and obtaining suspension cells, further simulate the natural growth environment of Moringa oleifera at high temperature and high light, and optimize the liquid compound high-sugar proliferation medium, choose high-concentration brown sugar instead of sucrose, and stimulate Moringa oleifera cells to synthesize Moringa polysaccharides during the culture process. Increase polysaccharide production. The method of the present invention can industrially produce Moringa polysaccharide, does not occupy arable land, has stable yield, high active ingredient content, and does not destroy natural resources. Compared with planting Moringa (production cycle 3 to 5 years), the production cycle of the present invention is shortened to 7-10 days, the production scale is easy to control, and the investment risk is small.

Description

technical field [0001] The invention relates to the technical field of natural product biochemistry, in particular to a method for producing Moringa oleifera polysaccharides by suspension culture of non-embryogenic cells of Moringa oleifera. Background technique [0002] Moringa, cauliflower Moringaceae, native to India, also known as the drumstick tree, is a perennial tropical deciduous tree, there are about 14 species in the world, currently the following three species are more commonly eaten: Indian traditional spicy Moringa, Indian improved species Moringa (improved species of T.N. Agricultural University in India, early growth and high pod yield) and African Moringa (originally only produced near Lake Turkana in Kenya and southwestern Ethiopia). The leaves and pods of Moringa contain various minerals, vitamins, 20 kinds of amino acids, 46 kinds of antioxidants, 36 kinds of natural anti-inflammatory agents and minerals. Studies have shown that the vitamin E content in 1...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A01H4/00
Inventor 蒋盛军张德旺
Owner 海南木辣达生物科技有限公司
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