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A protein molecular electronic device based on nanopore structure

A nanopore structure, electronic device technology, applied in specific-purpose bioreactors/fermenters, biochemical instruments, biomass post-processing, etc., can solve problems such as poor base sensitivity on nucleic acid chains

Inactive Publication Date: 2016-08-31
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention aims at the problem of poor sensitivity of the through-hole current to the bases on the nucleic acid chain in the solid-state nanopore sequencing technology. By immobilizing single protein molecules such as DNA polymerase or RNA polymerase or exonuclease in the nanopore, the efficiency of the through-hole is improved. The ability of current to recognize bases on single-stranded nucleic acids, and the development of a low-cost, fast DNA sequencing device

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  • A protein molecular electronic device based on nanopore structure

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Effect test

Embodiment approach 1

[0044] Embodiment 1: DNA polymerase molecular electronic device based on nanopore structure

[0045] (1) Preparation of nanopore chips: A series of micro-nano processing methods such as photolithography, dry etching, wet etching, and reactive ion beam etching are used to prepare Si / SiO 2 / SiN suspended support membrane structure. Among them, SiO 2 As an insulating buffer layer, it plays the role of supporting the SiN film and reducing the capacitive effect in electrical measurement. Si mainly supports SiO 2 effect. Finally, a 300kV high-energy focused electron beam (TEM) is used to process a nanohole with a diameter of less than 10 nanometers in the center of the suspended membrane. Processing indicators: nanopore diameter: less than 10 nanometers; thickness: around 50 nanometers.

[0046] (2) The self-made nanopore surface was modified with amino groups. The specific processing procedure is as follows: plasma treatment of self-made nanopore chip for 3 minutes, immersi...

Embodiment approach 2

[0053] Embodiment 2: RNA polymerase molecular electronic device based on nanopore structure

[0054] (1) Preparation of nanopore chips: A series of micro-nano processing methods such as photolithography, dry etching, wet etching, and reactive ion beam etching are used to prepare Si / SiO 2 / SiN suspended support membrane structure. Among them, SiO 2 As an insulating buffer layer, it plays the role of supporting the SiN film and reducing the capacitive effect in electrical measurement. Si mainly supports SiO 2 effect. Finally, a 300kV high-energy focused electron beam (TEM) is used to process a nanohole with a diameter of less than 10 nanometers in the center of the suspended membrane. Processing indicators: nanopore diameter: less than 10 nanometers; thickness: around 50 nanometers.

[0055] (2) The self-made nanopore surface was modified with amino groups. The specific processing procedure is as follows: the self-made nanopore chip is subjected to plasma treatment for 3...

Embodiment approach 3

[0062] Embodiment 3: Urease molecular electronic device based on nanopore structure

[0063] (1) Preparation of nanopore chips: A series of micro-nano processing methods such as photolithography, dry etching, wet etching, and reactive ion beam etching are used to prepare Si / SiO 2 / SiN suspended support membrane structure. Among them, SiO 2 As an insulating buffer layer, it plays the role of supporting the SiN film and reducing the capacitive effect in electrical measurement. Si mainly supports SiO 2 effect. Finally, a 300kV high-energy focused electron beam (TEM) is used to process a nanohole with a diameter of less than 10 nanometers in the center of the suspended membrane. Processing indicators: nanopore diameter: less than 10 nanometers; thickness: around 50 nanometers.

[0064] (2) The self-made nanopore surface was modified with amino groups. The specific processing procedure is as follows: plasma treatment of self-made nanopore chip for 3 minutes, immersion in aq...

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Abstract

The invention relates to a protein molecular electronic device based on a nanopore structure, which is a single-enzyme biosensor, which can be used to detect substrate molecules with extremely low concentration in solution, and can be used to sequence single-molecule DNA. The invention belongs to the third generation of DNA sequencing devices. A protein molecular electronic device based on a nanopore structure, characterized in that: 1) a solid structure with a nanochannel divides the solution into two parts, and the solutions on both sides of the solid structure communicate through the nanochannel; 2) in the nanochannel Immobilize protein molecules or protein complex molecules; 3) Place electrodes in the two solutions separated by the solid structure above, and detect the resistance of protein molecules passing through the nanochannel by detecting the current between the electrodes; 4) According to the current of protein molecules Value changes, determine the dynamic characteristics of protein conformation, and obtain information on the substrates of protein-catalyzed reactions.

Description

technical field [0001] The invention relates to a protein molecular electronic device based on a nanopore structure, which is a single-enzyme biosensor, which can be used to detect substrate molecules with extremely low concentration in solution, and can be used to sequence single-molecule DNA. The invention belongs to the third generation of DNA sequencing devices. Background technique [0002] DNA sequencing technology is one of the important milestones in the development of modern life sciences. In the past ten years, the emergence of low-cost, high-throughput DNA sequencing technology has caused the explosive growth of the largest and most core nucleic acid sequence information in the highly complex system of life, which has brought unprecedented opportunities for the development of life sciences and medicine. And challenges, the thorough deciphering of the genetic code of life will soon become possible, and the popularization of big data on genetic information will ben...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34
CPCB01J19/0046B01J2219/00277B01J2219/00608B01J2219/00853
Inventor 陆祖宏李清宁赵清贾忠伟万成康玉麟丁韬力
Owner PEKING UNIV
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