A kind of oil-based drilling cuttings degrading bacteria and its preparation and application method
A technology of oil-based drilling cuttings and degrading bacteria, which is applied in the direction of biochemical equipment and methods, chemical instruments and methods, and methods based on microorganisms, can solve the problems of long processing cycle and low degradation rate, and achieve short processing cycle and improved Degradation rate, effect of shortening degradation cycle
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[0025] The preparation of medium is listed in Table 1:
[0026] Table 1
[0027]
Embodiment 1
[0029] (1) Take petroleum-contaminated soil at the well site, carry out enrichment culture on the bacteria in the soil under the conditions of LB medium, neutral environment, and temperature at 30°C, and isolate, purify and screen the bacteria after enrichment culture, Three strains of degrading monobacteria were obtained, including Pseudomonas aeruginosa, Micrococcus krusei, and Acinetobacter calcoaceticus.
[0030] (2) Mix the three strains of degrading monobacteria obtained in step 1 according to the volume ratio of Pseudomonas aeruginosa: Micrococcus krusei: Acinetobacter calcium acetate 1:0.9:0.9 to obtain a mixed bacterial solution, in which oil-based drilling cuttings The viable count of degrading bacteria is 10 9 ~10 11 pcs / g.
[0031] (3) The mixed bacterial liquid of step (2) is fermented and amplified in the industrial medium P1, and the fermentation process conditions are: 33° C. of fermentation temperature, 10% v / v of inoculum size, 100 rpm of stirring speed, 24...
Embodiment 2
[0034] Oil-contaminated soil was taken at the well site, and the bacteria in the soil were enriched and cultured in LB medium, neutral environment, and temperature at 35°C. The bacteria after enrichment culture were separated, purified and screened to obtain 3 strains Degradative monobacteria, including Pseudomonas aeruginosa, Micrococcus krusei, and Acinetobacter calcoaceticus.
[0035] The same step as in Example 1, the difference is that Pseudomonas aeruginosa: Micrococcus krusei: Acinetobacter calcium acetate is mixed in a volume ratio of 1:1:1; the mixed bacteria solution is carried out in industrial medium P3 Fermentation scale-up cultivation; fermentation process conditions are: fermentation temperature is 35 ℃, inoculum size is 15% v / v, stirring speed is 120rpm, fermentation time is 36h, and the gas-liquid volume ratio of feeding air per minute is 1.2; after fermentation, Filter and separate the degrading bacteria through a plate and frame filter press with a pressure ...
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