Degradation bacterium for oil-base drilling cuttings, and preparation method and application method thereof
A technology for oil-based drilling cuttings and degrading bacteria, which is applied in biochemical equipment and methods, chemical instruments and methods, and microorganism-based methods, etc. The effect of short processing cycle and simple operation
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[0025] Preparation of media, listed in Table 1:
[0026] Table 1
[0027]
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[0028] Example 1:
[0029] (1) Take oil-contaminated soil from the well site, enrich and cultivate the bacteria in the soil under the conditions of LB medium, a neutral environment and a temperature of 30 °C, and separate, purify and screen the enriched bacteria. Three degrading bacteria were obtained, including Pseudomonas aeruginosa, Micrococcus cruzi, and Acinetobacter calcoaceticus.
[0030] (2) Mixing the three degrading single bacteria obtained in step 1 according to the volume ratio of Pseudomonas aeruginosa: Micrococcus cruzi: Acinetobacter calcoaceticus 1:0.9:0.9 to obtain a mixed bacterial solution, wherein oil-based drilling cuttings The bacterial count of degrading bacteria is 10 9 ~10 11 pieces / g.
[0031] (3) The mixed bacterial liquid of step (2) is fermented and amplified in the industrial medium P1, and the fermentation process conditions are: fermentation temperature 33 ° C, inoculum size 10% v / v, stirring speed 100 rpm, fermentation time 24h, fermentation...
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[0033] Example 2:
[0034] Oil-contaminated soil was taken from the well site, and the bacteria in the soil were enriched and cultured in LB medium, a neutral environment, and a temperature of 35 °C. The enriched bacteria were isolated, purified and screened to obtain 3 strains. Degrading single bacteria, including Pseudomonas aeruginosa, Micrococcus cruzi, and Acinetobacter calcoaceticus.
[0035] The steps are the same as those in Example 1, except that Pseudomonas aeruginosa: Micrococcus cruzi: Acinetobacter calcoaceticus is mixed in a volume ratio of 1:1:1; the mixed bacterial solution is carried out in the industrial medium P3. Fermentation scale-up culture; fermentation process conditions are: fermentation temperature is 35°C, inoculum volume is 15% v / v, stirring speed is 120rpm, fermentation time is 36h, and the volume ratio of air to liquid per minute is 1.2; The degrading bacteria were filtered and separated by a plate-and-frame filter press, and the filter pressure ...
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