Newborn total galactose detection kit, as well as application method and preparation method thereof

A detection kit and galactose technology, applied in the screening field of neonatal galactosemia, can solve the problems of high work intensity, poor screening accuracy of neonatal galactosemia, slow detection speed, etc.

Active Publication Date: 2015-01-28
GUANGZHOU FENGHUA BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to solve the problems of poor accuracy, slow detection speed and high work intensity in existing neonatal galactosemia screening

Method used

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  • Newborn total galactose detection kit, as well as application method and preparation method thereof
  • Newborn total galactose detection kit, as well as application method and preparation method thereof
  • Newborn total galactose detection kit, as well as application method and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Neonatal total galactose detection kit

[0059] Neonatal total galactose detection kit contains the following components: 1) Experimental buffer; 2) Mixed lyophilized powder of alkaline phosphatase and oxidized coenzyme II; 3) Lyophilized powder of galactose dehydrogenase; 4) Galactose Calibrator and quality control of filter paper dry blood film; 5) Copper reagent; 6) 96-well microwell blank white reaction plate.

[0060] Wherein, the experimental buffer is a Tris-HCl buffer containing KCl, wherein the molar concentration of Tris-HCl is 10 mmol / L, the molar concentration of KCl is 50 mmol / L, and the pH is 8.0.

[0061]The preparation method of the mixed freeze-dried powder of alkaline phosphatase and oxidized coenzyme II is: a) prepare the mixed solution of alkaline phosphatase and oxidized coenzyme II, which includes alkaline phosphatase with a concentration of 1000U / mL, and the concentration is 46mg / mL oxidized coenzyme II, Tris-HCl with a molar concentration of 1...

Embodiment 2

[0088] Neonatal total galactose detection kit

[0089] Neonatal total galactose detection kit contains the following components: 1) Experimental buffer; 2) Mixed lyophilized powder of alkaline phosphatase and oxidized coenzyme II; 3) Lyophilized powder of galactose dehydrogenase; 4) Galactose Calibrator and quality control of filter paper dry blood film; 5) Copper reagent; 6) 96-well microwell blank white reaction plate.

[0090] Wherein, the experimental buffer is Tris-HCl buffer containing KCl, wherein the molar concentration of Tris-HCl is 10 mmol / L, and the molar concentration of KCl is 10 mmol / L.

[0091] The preparation method of the mixed freeze-dried powder of alkaline phosphatase and oxidized coenzyme II is as follows: a) prepare the mixed solution of alkaline phosphatase and oxidized coenzyme II, wherein the concentration of alkaline phosphatase is 25U / mL, and the concentration of oxidized coenzyme II The concentration of coenzyme II is 1000mg / mL, the molar concen...

Embodiment 3

[0097] Neonatal total galactose detection kit

[0098] Neonatal total galactose detection kit contains the following components: 1) Experimental buffer; 2) Mixed lyophilized powder of alkaline phosphatase and oxidized coenzyme II; 3) Lyophilized powder of galactose dehydrogenase; 4) Galactose Calibrator and quality control of filter paper dry blood film; 5) Copper reagent; 6) 96-well microwell blank white reaction plate.

[0099] Wherein, the experimental buffer is a Tris-HCl buffer containing KCl, wherein the molar concentration of Tris-HCl is 100 mmol / L, and the molar concentration of KCl is 100 mmol / L.

[0100] The preparation method of the mixed freeze-dried powder of alkaline phosphatase and oxidized coenzyme II is as follows: a) prepare the mixed solution of alkaline phosphatase and oxidized coenzyme II, wherein the concentration of alkaline phosphatase is 5000U / mL, and the concentration of oxidized coenzyme II The concentration of coenzyme II is 10mg / mL, the molar co...

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Abstract

The invention discloses a newborn total galactose detection kit. The newborn total galactose detection kit comprises mixed freeze-dried powder, freeze-dried galactose dehydrogenase powder, galactose filter paper dried-blood spot calibration product, a quality control product, copper reagent and a white reaction board, wherein the mixed freeze-dried powder comprises an experiment buffer solution, alkaline phosphatase and oxidized form coenzyme II. The invention further discloses an application method and a preparation method of the newborn total galactose detection kit. The newborn total galactose detection kit is economical, has the time-saving and labor-saving effects, can be used for screening galactosemia of newborns, and lays a good technological base for the promotion of the screening for the newborns.

Description

technical field [0001] The present invention relates to the field of neonatal galactosemia screening, in particular to a kit for rapidly detecting neonatal total galactose based on the principle that total galactose reacts with oxidized coenzyme II to generate fluorescent substance reduced coenzyme II. Method of use and preparation method. Background technique [0002] Galactosemia is an autosomal recessive genetic disease caused by galactose utilization disorders and accumulation of harmful metabolites due to enzyme defects in the galactose metabolism process. It is relatively rare clinically, and the incidence rate varies in different regions. The incidence rate is 12 / 100,000 in eastern Saudi Arabia, 1 / 30,000 for permanent residents in Ireland, and about 1 / 59,000 in the United States. According to reports, the incidence rate in Hong Kong is 0.25 / 100,000. [0003] In the liver, galactose is expressed in galactokinase (galactokinase, GALK), galactose-1-phosphate uridyl-tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/66G01N33/531
CPCG01N33/66
Inventor 冯健明
Owner GUANGZHOU FENGHUA BIOENG
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