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Boar frozen semen diluted powder

A technology for freezing semen and glucose, which is applied in the field of cryopreservation of pig semen, and can solve the problems of blind purchase of dilution powder, lack of evaluation standards and institutions, and mixed results

Inactive Publication Date: 2015-02-04
SHANDONG XINJI ANIMAL HUSBANDRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The diluent powder market is relatively chaotic, with mixed fish and dragons, and there are no specialized evaluation standards and institutions, making producers blind when purchasing diluent powder

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment

[0015] D-glucose 11.5g, trisodium citrate 11.65g, EDTA sodium salt 2.35g, potassium dihydrogen phosphate 1.75g, potassium cyanide 0.75g, streptomycin sulfate 0.5g, polyvinyl alcohol (PVP.Type11) 1.0g , tricarboxymethylaminomethanol (Tris) 5.5g, citric acid 4.1g, semipanine 0.07g, OEP (amino-sodium dodecyl sulfate) 0.4g, SDS (sodium dodecyl sulfate) 0.3 g, PGF2a 0.5g, vitamin E 0.5g, GSH (glutathione) 0.5g, hyaluronic acid 0.3g, N-acetyl-D-glucosamine 0.5g add 1000g temperature at 37℃, containing 20 impurities Double distilled water within 100 mg can be used after mixing evenly.

[0016] The use effect of above-mentioned embodiment is shown in the following table:

[0017]

[0018] The present invention is a dilution powder specially used for freezing pig semen. Experimental data show that since the sugar in the diluent of the present invention is combined with monosaccharides and disaccharides, the integrity rate of the acrosome and the survival rate of sperm after freezin...

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Abstract

The invention discloses a boar frozen semen diluted powder, which is characterized in that: the diluted powder is composed of D-glucose, trisodium citrate, EDTA sodium salt, potassium dihydrogen phosphate, potassium cyanide, streptomycin sulphate, polyvinyl alcohol (PVP.Typell), trishydroxymethylaminomethanol (Tris), citric acid, cysteine, OEP (amino-sodium dodecyl sulfate), SDS (sodium dodecyl sulfate), PGF2a, vitamin E, GSH (glutathione), hyaluronic acid, and N-acetyl-D-aminoglucose. The boar frozen semen diluted powder provided by the invention improves the fertilization rate and litter size of boar frozen semen, simultaneously can improve the product safety, reduces the risk of semen infection caused by animal derived components in the diluent, and fills the domestic blank in the field, thus having far-reaching significance.

Description

technical field [0001] The invention belongs to the technical field of cryopreservation of porcine semen, in particular to a dilution powder of porcine frozen semen. Background technique [0002] In recent years, the research focus of artificial insemination of pigs has gradually shifted to freezing semen. Pig frozen semen technology refers to the use of dry ice (-79°C), liquid nitrogen (-196°C) as cooling sources, freezing the semen after special treatment, and storing it in a state of ultra-low temperature (-79°C or -196°C). The purpose of long-term preservation, and the technique of insemination after thawing to achieve a higher conception rate. China is the world's largest producer of pigs and pork. Especially in recent years, my country's pig industry is transforming from traditional extensive and free-range farming to large-scale and intensive feeding. Artificial insemination technology has been widely promoted and applied to pig production practice. However, in my c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 彭兴泉郎秀锦梁慧仉弦张旭杨军峰袁震
Owner SHANDONG XINJI ANIMAL HUSBANDRY
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