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A preparing method of a shake-flask culture medium

A culture medium preparation and culture medium technology, which is applied in the field of preparation of tetracycline shake flask culture medium, can solve the problems of large fluctuations in shake flask batches, single types of raw materials, affecting the quality and potency of shake flask fermentation, etc.

Inactive Publication Date: 2015-02-11
NINGXIA QIYUAN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the preparation of tetracycline shake flasks, the raw materials are single
Starch is gelatinized with boiling water during preparation, and the quality of the preparation medium is closely related to the temperature of water and the amount of water added during gelatinization; and after the medium is sterilized, the higher the starch ratio, the more viscous it is, and the long period of strain adjustment after inoculation is likely to cause The difference between batches of shake flasks fluctuates greatly, which affects the quality and potency of shake flask fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Add 60g of cornstarch and 700ml of drinking water to a 1000ml beaker, add 0.06g of amylase, place the beaker on an induction cooker, heat and stir, when the temperature rises to 70-80°C, keep it warm for 10-15 minutes , to produce liquefied sugar. Mix 20 grams of soybean cake powder, 10 grams of yeast powder, 5.0 grams (NH 4 ) 2 SO 4 , 6.0 g NaSO 4 , 1.2 g MgSO 4 .7H 2 O, 3.0 g K 2 HPO 4 .3H 2 O. Dissolve them in drinking water and add them to liquefied sugar, set the volume to 1000 ml, adjust to pH 7.0-7.2, pack into shake flasks, and sterilize at 118-121°C for 30 minutes. The tetracycline strain was placed in the shake flask culture medium, and after culturing for 160 hours, the detected fermentation unit was 20570u / ml, which was 21% higher than that of the comparative experiment.

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Abstract

The invention relates to a preparing method of a shake-flask culture medium, and particularly relates to a preparing method of a tetracycline shake-flask culture medium. The preparing method includes: preparing liquefied sugar from corn starch and amylase, adding soybean cake meal, yeast powder, (NH4)2SO4, Na2SO4, MgSO4.7H2O, and K2HPO4.3H2O in sequence, adding to a constant volume, adjusting pH with an alkaline reagent, and sterilizing to obtain the shake-flask culture medium. Based on traditional tetracycline shake-flask culture media, and by the preparing method in which the liquefied sugar is prepared firstly and then other raw materials of the culture medium are added, the fermentation tilter when the culture medium is used for tetracycline shake-flask culture is increased by 20-25% than the fermentation tilter when a traditional culture medium is used.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation medicine, in particular to a preparation method of a tetracycline shake flask culture medium. Background technique [0002] Tetracycline has the advantages of wide antibacterial spectrum, acuteness, low toxicity, and small side effects. Although it is not directly used in medicine, it is known for its antibacterial, growth-promoting, high feed utilization rate, and low residual amount in the human body. Advantages, it is widely used as feed additive in animal husbandry; in addition, semi-synthetic antibiotics with high clinical value and superior curative effect can be prepared or invented through chemical transformation. In recent years, tetracyclic antibiotics have been used in anti-tumor, inhibiting bone resorption, Research on bone formation and other aspects has broadened the scope of application of such products in medicine. At present, in the breeding of tetracycline strain...

Claims

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Application Information

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IPC IPC(8): C12P29/00
Inventor 张燕玉孙瑞君陈卓王惠
Owner NINGXIA QIYUAN PHARMA