Biological bactericide for preventing and controlling SRB (sulfate reducing bacteria) in high-temperature water body and SRB inhibition method of bactericide

A biological agent and high-temperature technology, applied in the direction of chemicals for biological control, botany equipment and methods, biocides, etc., can solve the problems of drug resistance, easy secondary pollution, less research, etc., to achieve Effects of eliminating sulfides, reducing production costs, and changing community structure

Active Publication Date: 2015-02-18
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical and physical methods are mainly used to control SRB and sulfide in oil wells, which have disadvantages such as high cost, easy secondary pollution, and formation of drug resistance.
[0003] Oilfield sulfate-reducing bacteria biological control gathering and transportation has gradually emerged in recent years. This technology mainly uses

Method used

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  • Biological bactericide for preventing and controlling SRB (sulfate reducing bacteria) in high-temperature water body and SRB inhibition method of bactericide
  • Biological bactericide for preventing and controlling SRB (sulfate reducing bacteria) in high-temperature water body and SRB inhibition method of bactericide
  • Biological bactericide for preventing and controlling SRB (sulfate reducing bacteria) in high-temperature water body and SRB inhibition method of bactericide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] JSHD-4 Geobacillus of the present invention is screened by the following process:

[0017] (1) Sample collection

[0018] The production fluids of 7 oil wells were collected in Jiangsu Oilfield. The sample names are: CH2-43, CH2-42, CH2-36, T83-1, T83-10, T83-7, H88-10.

[0019] (2) Culture medium configuration

[0020] The enrichment medium is composed of A solution and B solution, in which:

[0021] Solution A: 2.0 g inorganic salt K1, 1.0 g asparagine, 500 ml distilled water, pH 7.0~7.2;

[0022] Solution B: 8.5 g sodium citrate, 2 g sodium acetate, 2 g glucose, KH 2 PO 4 0.5 g, K 2 HPO 4 0.5 g, MgSO 4 ·7H 2 O 1.0 g, FeCl 3 ·6H 2 O 0.05 g, CaCl 2 0.1 g, yeast extract 0.5 g, distilled water 500 ml. The pH value is 7.0~7.2.

[0023] NRB liquid medium: liquid A and liquid B are sterilized separately at 121 ℃ for 20 min, and mixed in equal proportions after sterilization.

[0024] NRB solid medium: add agar with a mass ratio of 1.5% to 2% to liquid A and...

Embodiment 2

[0065] Inhibitory effect of JSHD-4 on SRB under laboratory system conditions

[0066] According to the formula of improved SRB medium in Table 3, the medium was prepared with sterile water as the solvent, the medium was sterilized at 121 °C for 20 min, and after cooling, trace element solutions were added to each liter of medium (the composition is shown in Table 4) 1 mL, 1 mL of vitamin solution (the composition is shown in Table 5), 0.1 g of ferrous ammonium sulfate sterilized by ultraviolet light and 0.5 g of L-cysteine ​​hydrochloride; The SRB strains (Desulfurizing Enterobacteriaceae) were inoculated into SRB medium in 3~4 loops, and cultured at 65°C for 48 hours to obtain freshly enriched cultured SRB strains.

[0067] At the same time, the preserved JSHD-4 was activated and cultured at 75 °C using NRB liquid medium (the same as the NRB liquid medium in Example 1) for 1 day to obtain a freshly enriched cultured JSHD-4 bacterial liquid, and then inoculated at 2% Ratio In...

Embodiment 3

[0073] First, the denitrifying bacteria JSHD-4 was prepared by conventional microbial aerobic fermentation to obtain a spore concentration of 1.0×10 6 cfu / mL industrial fermentation liquid, the industrial fermentation liquid can be stored in 20L plastic barrels, and then sodium nitrate, sodium nitrite and sodium dihydrogen phosphate are all commercially available industrial grade products, and they are mixed evenly in proportion when used, or they can be added in sequence Target oil well production fluid.

[0074] The JSHD-4 multifunctional biological bacterial agent of the present invention is put into Wang 39 well of Jiangsu Oilfield according to the following ratio, and the sulfide concentration of the output fluid of this well before treatment is 28mg / L. The dosage is: JSHD-4 industrial fermentation broth (1.0×10 6 cfu / mL), the dosage is 0.5% of the production fluid content; sodium nitrate: 20mg / L; sodium nitrite: 50mg / L; sodium dihydrogen phosphate: 5mg / L

[0075] When ...

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Abstract

The invention relates to a technology for inhibiting SRB (sulfate reducing bacteria) in oilfield produced liquid, in particular to a biological bactericide for preventing and controlling the SRB in a high-temperature water body and an SRB inhibition method of the bactericide. The biological bactericide is JSHD-4 geobacillustoebii, has a sequence as shown in a sequence table SEQ ID NO:1, is collected in China General Microbiological Culture Collection Center (CCMCC) on March 5, 2013, and has a Latin literature name of Geobacillustoebii and a collection number of CGMCCNO7273. The JSHD-4 geobacillustoebii is subjected to microbial aerobic fermentation to prepare industrial fermentation liquid with the bacterial solution concentration of 1.0*10<6> to 1.3*10<10> cfu/mL, the tolerable temperature is 50-95 DEG C, and the optimal growth temperature is 60-70 DEG C. After the multifunctional biological bactericide prepared from the JSHD-4 geobacillustoebii is thrown into a well, nitrate reducing bacteria (DNB) are quickly proliferated in the high-temperature environment in the well, so that the biological bactericide is suitable for directly treating the SRB in the high-temperature environment in the well on line.

Description

technical field [0001] The invention relates to the technical field of inhibiting sulfate-reducing bacteria in oilfield water, in particular to a biological agent for preventing and controlling SRB in high-temperature water and a method for inhibiting SRB. Background technique [0002] The problem of oil well corrosion has long plagued the oil exploration industry, and it is also a subject that oil well production and scientific research personnel have been paying attention to. It has not been well resolved so far. A large number of studies have shown that the biocorrosion of oil wells is mainly caused by the proliferation of harmful microorganisms sulfate reducing bacteria (SRB), which causes the increase of sulfide concentration. At present, chemical and physical methods are mainly used to control SRB and sulfide in oil wells, which have disadvantages such as high cost, easy secondary pollution, and formation of drug resistance. [0003] Oilfield sulfate-reducing bacteria...

Claims

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Application Information

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IPC IPC(8): C09K8/54C09K8/528A01N63/02A01P1/00E21B37/06E21B41/02
CPCA01N63/10C09K8/528C09K8/54C09K2208/32E21B37/06E21B41/02
Inventor 吴伟林杨帆姚峰薛芸孟章进姜桂英
Owner CHINA PETROLEUM & CHEM CORP
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