Unlock instant, AI-driven research and patent intelligence for your innovation.

Use of Isl1 gene or protein in Tetralogy of Fallot (TOF) diagnosis

A Tetralogy of Fallot, gene technology, applied in the direction of biochemical equipment and methods, microbe measurement/testing, material inspection products, etc., can solve problems such as cardiac hypoplasia

Pending Publication Date: 2015-02-25
SHANGHAI EAST HOSPITAL EAST HOSPITAL TONGJI UNIV SCHOOL OF MEDICINE
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, in the middle and late stages of heart development, it is still unclear which key gene deletion or low expression will not lead to embryonic death, but will lead to cardiac hypoplasia

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Use of Isl1 gene or protein in Tetralogy of Fallot (TOF) diagnosis
  • Use of Isl1 gene or protein in Tetralogy of Fallot (TOF) diagnosis
  • Use of Isl1 gene or protein in Tetralogy of Fallot (TOF) diagnosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] Example 1 Detailed phenotypic analysis of Isl1 mutant mice to elucidate the role of Isl1 in the formation of cardiac structures

[0100] method:

[0101] (1) Animal model

[0102] Mating Isl1hypo / hypo with Isl1+ / nLacZ or Isl1hypo / hypo will generate Isl1 compound low mutant mice (Isl1hypo / nLacZ) and low expression mutant mice, respectively. TnT-Cre;Isl1 f / + Mating with Isl1f / f mice will generate cardiomyocyte-specific Isl1 knockout mouse variants. Embryos were collected at different time points.

[0103] (2) Histological analysis: Mice were crossed, embryos were harvested at the desired developmental stages, and fixed with 4% PFA for whole-body and section staining analysis. For high-resolution tissue analysis, whole-mount embryos were paraffin-embedded and sectioned, and stained with Nuclear Fast Red. To analyze the morphology of the pharyngeal arch arteries, both transverse and longitudinal sections will be analyzed.

[0104] (3) Immunostaining and Cell Prolifera...

Embodiment 2

[0107] Example 2 Molecular Mechanism of Isl1 in the Process of Cardiac Outflow Tract Formation

[0108] (1) RNA in situ hybridization. Using Digoxigenin-labeled cRNA probe and AP-labeled Digoxigenin antibody (Roche Company), BCIP and NBT (Roche Company) kits were used for color development. The probes used include: BMP, TGF, FGF, Wnts, Shh, Notch, endothelins, PDGF, Tbx1, Tbx3 / 5, GATA factors, Nkx2.5, Mef2c, Foxh1, etc.

[0109] (2) Gene chip analysis: According to the type of change in outflow tract vascular phenotype and the time point of appearance, the time point of collection of outflow tract specimens will be determined, and the control, TnT-Cre; Isl1 variant and Isl1 low expression variation will be collected Mouse outflow tract specimens. The cardiac outflow tract samples were immediately placed in RNAlater and kept overnight at 4°C, and the RNAlater was removed the next day and stored at -80°C. RNA was extracted with Trizol. cRNA synthesis, labeling, microarray hy...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a use of an Isl1 gene or protein in Tetralogy of Fallot (TOF) diagnosis. Concretely, the invention discloses the use of the LIM homeodomain transcription factor (Islet1, ISl1) gene or protein in preparation of a reagent or a kit for prediction or diagnosis of TOF. The Isl1 gene or protein can be used for prenatal examination or children patient congenital heart disease early-screening and realizes sound child rearing or early intervention guide.

Description

technical field [0001] The present invention relates to the field of diagnosis of congenital heart disease, in particular, to the prediction or diagnosis of tetralogy of Fallot in prenatal screening. Background technique [0002] Congenital heart disease (CHD) is the most common congenital disorder, affecting up to 1% of newborns. 10% of neonatal deaths and 20% of early spontaneous abortions are directly related to congenital heart disease. [0003] At present, prenatal screening for congenital heart disease is often carried out around 23-24 weeks of pregnancy, and artificial abortion can be performed for severe congenital heart disease, but abortion at this time will cause greater harm to the mother; in addition, prenatal screening that relies on ultrasound Congenital heart screening has a certain rate of missed detection, and children with congenital heart disease born in this way bring a great burden to the family and society. [0004] Abnormal development of the great ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68G01N33/68
CPCC12Q1/6883C12Q2600/158G01N33/6893G01N2333/4703G01N2800/32
Inventor 梁兴群孙云甫
Owner SHANGHAI EAST HOSPITAL EAST HOSPITAL TONGJI UNIV SCHOOL OF MEDICINE