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Material capable of regulating cell adhesion

A technology of cells and substrate materials, applied in the field of biomedical materials, can solve problems such as being unsuitable for large-scale production, functional differences in bioactive molecules, and complex methods

Active Publication Date: 2015-03-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] There are many deficiencies in the current existing methods, for example: 1. The method is relatively complicated and cannot be generally applied to the surface of a variety of material substrates; 2. In practical applications, the stability and activity of bioactive molecules cannot be well maintained; 3. The functionality of different batches of bioactive molecules will vary
4. The addition of bioactive molecules is prone to inflammatory reactions; 5. Not suitable for large-scale production

Method used

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  • Material capable of regulating cell adhesion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 316L stainless steel is selected as the base material, and the polyelectrolyte assembly coating is constructed on the surface of the base material, including the following steps:

[0034] (1) Dissolving polylysine in a buffer solution with pH=7.4 to prepare a 1 mg / mL polylysine solution; dissolving heparin in a buffer solution with pH=7.4 to prepare a 1 mg / mL heparin solution;

[0035] (2) Soak the stainless steel in the polylysine solution completely, take it out after a period of time, and wash it with the buffer solution of pH=7.4;

[0036] (3) Soak the base material obtained in step (2) completely in the heparin solution, take it out after a period of time, and use a buffer solution with pH=7.4;

[0037] (4) Steps (2) to (3) were repeated 12 times to obtain a coating with a thickness of 800 nm.

[0038] When endothelial cells are cultured on the coating, endothelial cells can partially adhere to the surface of the coating, and cells can easily form cell clusters. ...

Embodiment 2

[0040] Polyethylene terephthalate (PET) is selected as the base material, and a polyelectrolyte assembly coating is constructed on the surface of the base material, including the following steps:

[0041] (1) dissolving chitosan in the buffer solution of pH=4.4 is made into 1mg / mL chitosan solution; Dissolving hyaluronic acid in the buffer solution of pH=7.4 is made into 1mg / mL hyaluronic acid solution;

[0042] (2) Soak the PET in the chitosan solution, take it out after a period of time, and wash with the buffer solution of pH=7.4;

[0043] (3) Soak the base material obtained in step (2) in the hyaluronic acid solution, take it out after a period of time, and use a buffer solution with pH=7.4;

[0044] (4) Repeat steps (2) to (3) 8 times to obtain a coating with a thickness of 200nm

[0045] When mesenchymal stem cells are cultured on the coated surface, the cells maintain low adhesion on the coated surface, while the cells cannot adhere to the surface of the uncoated materia...

Embodiment 3

[0047] 316L stainless steel is selected as the base material, and the coating is constructed on the surface of the base material, including the following steps:

[0048] (1) dissolving protamine sulfate in pure water to make 1mg / mL protamine sulfate solution; dissolving protamine deoxyribonucleic acid in pure water to make 1mg / mL deoxyribonucleic acid solution;

[0049] (2) Soak the polydimethylsiloxane substrate in the protamine sulfate solution, take it out after a period of time, and wash it with pure water;

[0050] (3) Soak the base material obtained in step (2) in the deoxyribonucleic acid solution, take it out after a period of time, and wash it with pure water;

[0051] (4) Repeat step (2) to step (3) 12 times, the thickness of the coating is 360nm.

[0052] When endothelial cells and smooth muscle cells were cultured simultaneously on the coated surface, endothelial cells could adhere, but smooth muscle cells could not. On the surface of pure polydimethylsiloxane, b...

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Abstract

The invention discloses a material capable of regulating cell adhesion. The material consists of a substrate material and a coating. A preparation method of the coating comprises the following steps: (1) respectively preparing a polycationic electrolyte solution and a polyanionic electrolyte solution; (2) soaking the substrate material into the polycationic electrolyte solution, taking out the substrate material, and removing the polycationic electrolyte solution by washing; (3) soaking the substrate material obtained in the step (2) into the polyanionic electrolyte solution, taking out the substrate material, and removing the polyanionic electrolyte solution by washing; and (4) repeating the steps (2) and (3) for a plurality of times to obtain a polyelectrolyte assembly coating, wherein the thickness of the coating is 1 nanometer to 10 microns. The material disclosed by the invention can be used for regulating the adhesion degree of cells without loading bioactive factors, and can be used for changing the adhesion degree of the cells around the material; and compared with the prior art, the material disclosed by the invention is safer and more stable.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a material capable of regulating cell adhesion. Background technique [0002] Drug-loaded supercoatings are constructed on the surface of medical devices and implants, and the functions of disease treatment, prevention, antibacterial, and interface biocompatibility can be improved through drug release. [0003] The layered assembly method constructs supercoatings through the alternate adsorption of two interacting substances on the substrate, which has the advantages of simple operation, mild conditions, and no selectivity to the substrate. As a technology for constructing functional coatings, this method has been widely and deeply researched and applied in many frontier fields such as biomedicine, optoelectronic components, and artificial intelligence. [0004] Polyelectrolytes, also known as polymer electrolytes, are a class of linear or branched synthetic and natural water...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/12A61L31/10A61L27/40A61L27/34C12N5/071
Inventor 计剑任科峰常皓
Owner ZHEJIANG UNIV