Method for catalyzed degradation of triazophos by chloroperoxidase

Abstract

The invention discloses a method for catalyzing and degrading triazophos through chloroperoxidase. The method comprises the following steps: based on a biological enzyme catalytic degradation system, adding the chloroperoxidase into a triazophos solution to be degraded, uniformly mixing, regulating pH of mixed liquid to 2-5 by using a phosphate buffer solution, then taking H2O2 as an oxidizing agent, adding an H2O2 aqueous solution into the mixed liquid, stirring at the room temperature and degrading the triazophos. The method has the benefits that the operation is simple, the using amounts of the chloroperoxidase and the H2O2 are less, and the reaction conditions are mild; the molecular structure of the triazophos can be quickly damaged, so that the triazophos is cracked into micromolecules, and accordingly, environmental pollution is reduced; the degradation rate of the triazophos under optimal conditions within 1-2 minutes can reach 98.3-100 percent.

Description

technical field [0001] The invention belongs to the technical field of water, waste water or sewage treatment, and in particular relates to a method for rapidly degrading triazophos by using an enzyme-catalyzed oxidation method. Background technique [0002] The pure product of triazophos is a light yellow oily liquid, which is a broad-spectrum phosphorothioate insecticide and acaricide, belonging to organophosphorus pesticides. Triazophos has strong permeability to rice plants, high stomach poisoning activity and ovicidal ability to pests. Therefore, it has strong contact activity against common rice pests such as rice stem borer and rice stem borer, and has been widely used in the control of rice, cotton and other pests. However, triazophos is toxic to bees and fish, and is more toxic to higher animals. High concentrations of triazophos may also have a strong mutagenic effect. With the increasing use of triazophos, while ensuring the yield of crops, it also has a negati...

AI Technical Summary

Problems solved by technology

Among them, soil adsorption and degradation is a physically reversible process. After the pesticide is applied to the soil, desorption may occur; hydraulic cavitation technology is still in the exploratory stage in the field of wastewater treatment, and the degradation efficiency of triazophos is only about 50%. It takes a long time for phosphorus-degrading bacteria to degrade triazophos (>1 hour); the degradation rate of freshwater algae is less than 50% within 8 days; the photocatalytic oxidation of diphenylthiophene takes a long time, and the degradation rate is only 73.96% in 6 days; TiO 2 Photocatalytic method requires a special light source with high cost, and the utilization rate of sunlight is low; Fenton oxidation method will produce secondary pollution when degraded; O 3 O 3 It needs to be generated online, and the equipment requirements are high