InDel molecular marker for basic resistance of rice to rice blast germs and application of InDel molecular marker
A technology of molecular markers and rice blast fungus, applied in the direction of recombinant DNA technology, DNA / RNA fragments, etc., can solve the problems of no polymorphism, lack of specific markers, and the inability of dominant markers to distinguish homozygotes and heterozygotes, etc., to achieve The effect of improving accuracy and efficiency and overcoming the influence of environmental conditions
Inactive Publication Date: 2015-03-11
YUNNAN AGRICULTURAL UNIVERSITY
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Problems solved by technology
Many current molecular markers have their own shortcomings, such as RAPD markers are dominant markers that cannot distinguish homozygotes and heterozygotes, SSR technology lacks general specific markers among varieties and polymorphisms are not as abundant as InDel markers. , so polymorphism-rich InDel markers have been widely used in the polymorphism analysis of Magnaporthe grisea
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[0016] The scheme of the present invention is described in further detail below in conjunction with the accompanying drawings and specific embodiments:
[0017] The rice variety used in the present invention is Moon Valley, a traditional local variety in Yunnan. This variety is a rice variety with medium resistance. After inoculating the rice variety with a strong pathogenic strain of Magnaporthe grisea, the total RNA of rice is extracted to obtain the transcript of Moon Valley infected by Magnaporthe oryzae. Microarray, through a large number of analysis, found that the expression of the gene Os01g0750500 was up-regulated when Moon Valley was infected by Magnaporthe grisea for 12 hours. Utilize the InDel molecular marker InDelBR1 used in the present invention to amplify the rice gene Os1g0750500 and the specific steps are as follows:
[0018] (1) Genomic DNA of a resistant rice variety Lijiang Xintuan Heigu and a susceptible rice variety Xieyou 527 were extracted
[0019] (2...
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The invention discloses an InDel molecular marker for basic resistance of rice to rice blast germs and application of the InDel molecular marker and the InDel molecular marker can be used for identifying the basic resistance to rice blast germs. The nucleotide sequence of the InDel molecular marker is shown in SEQ ID No.1. A primer pair of the InDel molecular marker can be adopted for PCR amplification by taking rice genome DNA as a template, the InDel molecular marker which is tightly linked with the basic resistance genes of rice to rice blast germs can be obtained, and the marker is named as InDelBR1 in the invention. Due to PCR amplification analysis, whether the rice has the basic resistance to the rice blast germs or not can be definitely judged, and whether the rice has the basic resistance to the rice blast germs or not can be detected. The InDel molecular marker has the characteristics of simple banding pattern, relatively dense distribution and the like, has unparallel advantages when being compared with SSR, is capable of rapidly and precisely detecting the polymorphism of subspecies, and has a wide application prospect.
Description
technical field [0001] The invention relates to the field of biomolecular markers, in particular to an Indel molecular marker for basic resistance of rice to blast fungus and application thereof. Background technique [0002] DNA molecular markers are DNA fragments that can reflect certain differences in the genome between individuals and populations. This DNA fragment is detected on electrophoresis gel or hybridization membrane after restriction endonuclease cutting and molecular hybridization or PCR amplification of genomic DNA. It is widely used in biological genome research, evolutionary classification, genetic breeding and so on. DNA molecular markers have the following advantages: ① Not affected by the environment and there is no question of gene expression or not; ② Abundant in quantity, covering the entire genome; ③ High polymorphism; sex etc. Currently widely used molecular markers include RFLP (Restriction Fragment Length Polymorphism), AFLP (Amplified Fragment ...
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IPC IPC(8): C12N15/11C12Q1/68
Inventor 杨静李成云朱有勇刘林梁美玲
Owner YUNNAN AGRICULTURAL UNIVERSITY