Conjugated linoleic acid isomer biological enrichment method

Abstract

A conjugated linoleic acid isomer biological enrichment method includes preparation of a biological enrichment culture medium, preparation of production strains, conjugated linoleic acid isomer biological enrichment and other steps, and c9,t11-conjugated linoleic acid (CLA) isomers can be obtained. The c9,t11-conjugated linoleic acid (CLA) isomer content is greater than 0.45% by mass, substrate conversion rate is more than 90%, the c9,t11-conjugated linoleic acid (CLA) isomer content in products is increased significantly, by biological enrichment culture, not only an anti-cancer function product rich in the c9,t11-conjugated linoleic acid (CLA) isomer can be obtained, proteins and celluloses in defatted soybean also can be decomposed into polypeptides and soluble dietary fiber and other health care function active factors, the health care function of the products is significantly enhanced, and the application range of the product is further broadened.

Description

technical field

[0001] The invention belongs to the technical field of biomedicine. Background technique

[0002] Conjugated linoleic acid (CLA) is a general term for a group of octadecyl-conjugated dienoic acids, and the conjugated double bond has 4 positions of isomerism (8,10; 9,11; 10,12; 11 ,13) and 4 geometric isomers ( cis , cis ; cis , trans ; trans , cis ; trans , trans ), there are as many as 16 isomers, such as: c 9, t 11-CLA and t 10, c 12-CLA isomers, etc. Studies have found that CLA has physiological functions such as anti-cancer, preventing atherosclerosis, enhancing body immunity, and anti-diabetes, and more and more studies have shown that its physiological activities are isomer-specific, and it is now generally believed that c 9, t 11-CLA isomers have anticancer and atherosclerotic effects. In nature, CLA is mainly found in the milk and fat of ruminants, mainly composed of c 9, t 11-CLA and small amounts t 9, t 11-CLA, t 10, c 11-CL...

Examples

Embodiment 1

[0020] After soaking 1 part of low-temperature defatted soybeans with 4 parts of water overnight, coarsely grind and then finely grind 1 time with a refiner, and then process 2 times with a colloid mill to obtain a mixed emulsion. After boiling, adjust the protein content therein to The mass percentage is 8% to obtain the basal medium. Add 1% fructo-oligosaccharides by mass to the basic medium, and pasteurize to obtain a bio-enriched medium. Insert 5% Lactobacillus casei ( Lactobacillus casei ) CGMCC 1.574 production strain, and at the same time add 0.5% linoleic acid substrate, mix well and incubate at 37°C for 24h. After enrichment by this method, the resulting CLA isomers are c 9, t 11-CLA isomers ( figure 1 ), in the fermented product c 9, t The content of 11-CLA isomer is 0.46% by mass, and the conversion rate of linoleic acid is 92%. The concentration of the substrate linoleic acid has a significant impact on the synthesis of the product CLA. Under optimal condi...

Embodiment 2

[0022] After soaking 1 part of low-temperature defatted soybeans with 4 parts of water for 6 hours, coarsely grind and then finely grind once with a refiner, and then process twice with a colloid mill to obtain a mixed emulsion, after boiling, adjust the protein content in it to The mass percentage is 5%, and the skimmed milk powder with a mass percentage of 3% is added to obtain a basal medium. Add 1% fructo-oligosaccharides by mass to the basic medium, and pasteurize to obtain a bio-enriched medium. Insert 5% Lactobacillus casei ( Lactobacillus casei ) CGMCC 1.574 production strains, at the same time add 0.5% linoleic acid substrate, mix well and incubate at 37°C for 24h, the product c 9, t The content of 11-CLA isomer is 0.48% by mass. After the fermentation product is spray-dried or freeze-dried at low temperature, it is processed into tablets by pressing and coating, which can effectively prevent c 9, t The 11-CLA isomer undergoes an oxidation reaction during storag...

technical field

[0001] The invention belongs to the technical field of biomedicine. Background technique

[0002] Conjugated linoleic acid (CLA) is a general term for a group of octadecyl-conjugated dienoic acids, and the conjugated double bond has 4 positions of isomerism (8,10; 9,11; 10,12; 11 ,13) and 4 geometric isomers ( cis , cis ; cis , trans ; trans , cis ; trans , trans ), there are as many as 16 isomers, such as: c 9, t 11-CLA and t 10, c 12-CLA isomers, etc. Studies have found that CLA has physiological functions such as anti-cancer, preventing atherosclerosis, enhancing body immunity, and anti-diabetes, and more and more studies have shown that its physiological activities are isomer-specific, and it is now generally believed that c 9, t 11-CLA isomers have anticancer and atherosclerotic effects. In nature, CLA is mainly found in the milk and fat of ruminants, mainly composed of c 9, t 11-CLA and small amounts t 9, t 11-CLA, t 10, c 11-CL...

Examples

Embodiment 1

[0020] After soaking 1 part of low-temperature defatted soybeans with 4 parts of water overnight, coarsely grind and then finely grind 1 time with a refiner, and then process 2 times with a colloid mill to obtain a mixed emulsion. After boiling, adjust the protein content therein to The mass percentage is 8% to obtain the basal medium. Add 1% fructo-oligosaccharides by mass to the basic medium, and pasteurize to obtain a bio-enriched medium. Insert 5% Lactobacillus casei ( Lactobacillus casei ) CGMCC 1.574 production strain, and at the same time add 0.5% linoleic acid substrate, mix well and incubate at 37°C for 24h. After enrichment by this method, the resulting CLA isomers are c 9, t 11-CLA isomers ( figure 1 ), in the fermented product c 9, t The content of 11-CLA isomer is 0.46% by mass, and the conversion rate of linoleic acid is 92%. The concentration of the substrate linoleic acid has a significant impact on the synthesis of the product CLA. Under optimal condi...

Embodiment 2

[0022] After soaking 1 part of low-temperature defatted soybeans with 4 parts of water for 6 hours, coarsely grind and then finely grind once with a refiner, and then process twice with a colloid mill to obtain a mixed emulsion, after boiling, adjust the protein content in it to The mass percentage is 5%, and the skimmed milk powder with a mass percentage of 3% is added to obtain a basal medium. Add 1% fructo-oligosaccharides by mass to the basic medium, and pasteurize to obtain a bio-enriched medium. Insert 5% Lactobacillus casei ( Lactobacillus casei ) CGMCC 1.574 production strains, at the same time add 0.5% linoleic acid substrate, mix well and incubate at 37°C for 24h, the product c 9, t The content of 11-CLA isomer is 0.48% by mass. After the fermentation product is spray-dried or freeze-dried at low temperature, it is processed into tablets by pressing and coating, which can effectively prevent c 9, t The 11-CLA isomer undergoes an oxidation reaction during storag...

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