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Kit for rapid detection and quantification of thiocyanate radicals in raw fresh milk, and its application

A technology of thiocyanate and reagent kits, which is applied in the direction of analyzing materials through chemical reactions, and analyzing materials through observing the impact on chemical indicators, so as to save resources, improve detection efficiency, and improve on-site detection efficiency.

Inactive Publication Date: 2015-03-11
黑龙江省兽药饲料监察所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Some of the existing kits that can be used for on-site detection are mainly qualitative, and can only perform qualitative screening on whether thiocyanate is contained in raw milk, and perform an intuitive detection of the concentration of thiocyanate according to the detection limit provided by it. identify

Method used

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  • Kit for rapid detection and quantification of thiocyanate radicals in raw fresh milk, and its application
  • Kit for rapid detection and quantification of thiocyanate radicals in raw fresh milk, and its application
  • Kit for rapid detection and quantification of thiocyanate radicals in raw fresh milk, and its application

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specific Embodiment approach 1

[0027] Specific implementation mode one: as Figure 1-5 As shown, the rapid detection and quantification kit for thiocyanate in fresh milk provided in this embodiment consists of 2 graduated straws, colorimetric tubes with lids (24 pieces), protein precipitant (15-20mg / ml trichloroacetic acid aqueous solution ), chromogen (1-2mg / ml ferric chloride hydrochloric acid solution), thiocyanate reference substance (1.6732g), and sample color guide, wherein: the sample color guide is a white bottom plate with 4 rows of small holes, each row 6 pieces, the protein precipitating agent and the color developing agent are packed in a light-proof glass bottle with a stopper, and the potassium thiocyanate reference substance is packed in a dry and light-proof aluminum foil sealed packet.

[0028] The above kit can be used to detect the content of thiocyanate in fresh milk, and the specific detection method is carried out according to the following steps:

[0029] 1) To prepare a standard con...

specific Embodiment approach 2

[0035] Specific implementation mode two: according to the method specified by the Ministry of Health: No. [2009] No. 29 of the Ministry of Health Food Remediation Office specifies the detection method of thiocyanate in milk, and the specific steps are as follows:

[0036] 1) To prepare a thiocyanate standard concentration gradient solution, take the potassium thiocyanate reference substance packet included in the kit and distill it to volume in a 1000ml volumetric flask to obtain a 1000ppm thiocyanate standard stock solution. Take 1 mL of thiocyanic acid standard stock solution, place it in a 100 mL volumetric flask, and add water to the mark. This solution contains thiocyanic acid 10mg / L. Pipette 0.1, 0.2, 0.5, 1.0, 2.0mL thiocyanic acid standard intermediate solution, dilute it with water into a 10mL volumetric flask, and make standard concentration gradient solutions with concentrations of 0.1, 0.2, 0.5, 1.0, 2.0mg / L respectively. Take six colorimetric tubes with lids, add...

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Abstract

The invention discloses a kit for rapid detection and quantification of thiocyanate radicals in raw fresh milk. The kit can be used for detecting the content of thiocyanate radicals in the raw fresh milk. The kit is composed of a measuring pipette, a colorimetric tube with a cover, a protein precipitating agent, a color developer, a thiocyanate radical reference substance and a sample color board. The rapid detection and quantification kit is characterized in that trichloroacetic acid is added to the raw fresh milk, the obtained fresh milk is shaken to precipitate proteins in the raw fresh milk, thiocyanate radicals in a supernatant obtained after standing and the ferric ion color developer can form colored ferric thiocyanate, different standard concentrations of a thiocyanate radical solution are prepared and react with iron trichloride, a standard color board is made according to displayed different chromas, the color of a sample to be detected is compared with a standard color board to determine the content of thiocyanate radicals in liquid milk, and the sample with a required concentration is operated on a machine in a lab. The detection limit of a method is 0.01%.

Description

technical field [0001] The invention relates to a kit for rapid detection and quantification of thiocyanate in fresh milk and its application. Background technique [0002] The cyanide ion released by thiocyanate in the body can make the tissue unable to use oxygen and cause poisoning, and can hinder the body's absorption of iodine and cause thyroid disease. In the breeding process of dairy cows, feed and water quality have an impact on the content of thiocyanate ions in raw milk. Adding thiocyanate into raw milk up to 15mg / kg can effectively inhibit bacteria, preserve freshness, and prolong the shelf life of the product. The problem of preservation quality. The "List of Non-usable Substances and Food Additives That May Be Added Illegally in Food (First Batch)" issued by the Ministry of Health clearly stipulates that thiocyanate in milk and dairy products is an illegally added substance. There is no standard for the detection of thiocyanate in our country, and there are m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 刘全宇郭文欣韩宏超许谨郭蔚
Owner 黑龙江省兽药饲料监察所
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