Method for determining aflatoxin

An aflatoxin and gold nanoparticle technology, which is applied in the fields of analytical chemistry and chemiluminescence, can solve the problems of insufficient sensitivity and specificity, insufficient simple and fast detection, insufficient reagent stability, etc., and achieves wide application prospects and simple and fast detection. , detect the effect of a wide linear range
CN104422686AInactive Publication Date: 2015-03-18QINGDAO LANNONGGU AGRI PROD RES & DEV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
QINGDAO LANNONGGU AGRI PROD RES & DEV
Publication Date
2015-03-18
Estimated Expiration
Not applicable · inactive patent
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Abstract

The invention discloses a method for determining aflatoxin. The method is characterized by comprising the following steps: (1) preparing a solution 1 for later use; (2) preparing a solution 2 for later use; (3) preparing R1 modified by HRP (horseradish peroxidase); (4) preparing R2 modified by HRP; and (5) detecting aflatoxin. Red rhodamine B can neither cause concentration quenching, nor cause insufficient light intensity. By adding mica powder, the luminescence lifetime of an oxalate luminescence system is significantly prolonged. Ruthenium terpyridine and chemiluminescence have the advantages of high detection sensitivity, wide linear range and the like, and aflatoxin can be detected more effectively. The method disclosed by the invention has the excellent properties of lower toxicity, higher flash point and lower vapor pressure. By detecting the content of aflatoxin in liquid milk and using a standard addition recovery method, the recovery yield of 98.4%-100.4% is obtained, indicating that the method has good accuracy in determination of aflatoxin.
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Description

technical field

[0001] The invention belongs to the field of analytical chemistry and chemiluminescence, and in particular relates to a method for determining aflatoxin. Background technique

[0002] Aflatoxins (AFT) are a class of compounds with similar chemical structures, all of which are derivatives of dihydrofuranocoumarins. Aflatoxins are secondary metabolites mainly produced by the fungus Aspergillus parasiticus, and the probability of aflatoxins appearing in food and feed in hot and humid areas is the highest.

[0003] The sudden deaths of 100,000 turkeys in the UK in the 1960s were identified as being linked to peanut meal imported from Brazil. Further investigation proved that the meal was contaminated with a poisonous substance from a fungus. These research work eventually It made people discover the toxic metabolites produced by Aspergillus flavus. Aflatoxins are metabolites of Aspergillus flavus and Aspergillus parasiticus. Aspergillus can also produce af...

Claims

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