Method for determining aflatoxin

An aflatoxin and gold nanoparticle technology, which is applied in the fields of analytical chemistry and chemiluminescence, can solve the problems of insufficient sensitivity and specificity, insufficient simple and fast detection, insufficient reagent stability, etc., and achieves wide application prospects and simple and fast detection. , detect the effect of a wide linear range

Inactive Publication Date: 2015-03-18
QINGDAO LANNONGGU AGRI PROD RES & DEV
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AI Technical Summary

Problems solved by technology

[0010] The disadvantages of the chemiluminescence substrate system in the currently commercially available chemiluminescence detection kits are: the detection sensitivity and specificity are not high enough, the reagent stability is not enough, the cost is high, the detection is not simple and fast enough, etc.

Method used

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Embodiment Construction

[0022] (1) Preparation of ready-to-use solution 1: After washing the magnetic gold nanoparticles with phosphate buffer, take 0.5×10 -5 M of ruthenium terpyridine, add 250 μl of 0.1×10 pH value of 9-10 -5 In the sodium borate buffer solution of M, react at 37 DEG C for 15h; Gained functionalized magnetic gold nanoparticles are washed with phosphate buffer, then dispersed in phosphate buffer, and stored at 5 DEG C for subsequent use;

[0023] (2) Preparation of ready-to-use solution 2: After washing the magnetic gold nanoparticles with phosphate buffer, add 150 μl of 2.0×10 -5 Bis(2,4-dinitrophenyl) oxalate of M, 1.0×10 -5 M bis(2,5-dinitrophenyl) oxalate, add 50 μl of 0.06mg / μl 350 mesh master powder and 50 μl of 0.5×10 -5 Red Rhodamine B of M. Reaction at 37°C for 15h; the resulting functionalized magnetic gold nanoparticles were washed with phosphate buffer, then dispersed in phosphate buffer, stored at 5°C for subsequent use;

[0024] (3) Preparation of HRP (horse...

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Abstract

The invention discloses a method for determining aflatoxin. The method is characterized by comprising the following steps: (1) preparing a solution 1 for later use; (2) preparing a solution 2 for later use; (3) preparing R1 modified by HRP (horseradish peroxidase); (4) preparing R2 modified by HRP; and (5) detecting aflatoxin. Red rhodamine B can neither cause concentration quenching, nor cause insufficient light intensity. By adding mica powder, the luminescence lifetime of an oxalate luminescence system is significantly prolonged. Ruthenium terpyridine and chemiluminescence have the advantages of high detection sensitivity, wide linear range and the like, and aflatoxin can be detected more effectively. The method disclosed by the invention has the excellent properties of lower toxicity, higher flash point and lower vapor pressure. By detecting the content of aflatoxin in liquid milk and using a standard addition recovery method, the recovery yield of 98.4%-100.4% is obtained, indicating that the method has good accuracy in determination of aflatoxin.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and chemiluminescence, and in particular relates to a method for determining aflatoxin. Background technique [0002] Aflatoxins (AFT) are a class of compounds with similar chemical structures, all of which are derivatives of dihydrofuranocoumarins. Aflatoxins are secondary metabolites mainly produced by the fungus Aspergillus parasiticus, and the probability of aflatoxins appearing in food and feed in hot and humid areas is the highest. [0003] The sudden deaths of 100,000 turkeys in the UK in the 1960s were identified as being linked to peanut meal imported from Brazil. Further investigation proved that the meal was contaminated with a poisonous substance from a fungus. These research work eventually It made people discover the toxic metabolites produced by Aspergillus flavus. Aflatoxins are metabolites of Aspergillus flavus and Aspergillus parasiticus. Aspergillus can also produce af...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N1/28
Inventor 宋波
Owner QINGDAO LANNONGGU AGRI PROD RES & DEV
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