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Method of improving stability of finished enramycin premixing agent

A technology of enramycin and premix, which is applied in the directions of non-active ingredients medical preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc. Enramycin quality and other issues, to achieve the effect of stable quality, convenient transportation and application, and low production cost

Active Publication Date: 2015-03-25
江西兴鼎科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although enramycin has a good development prospect in China, the finished product of enramycin premix is ​​easy to degrade, and its instability affects the quality of enramycin in the process of storage, transportation and application, which in turn affects its quality. sale and use

Method used

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  • Method of improving stability of finished enramycin premixing agent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for improving the stability of the enramycin premix finished product is achieved through the following measures:

[0032] (1) Fermentation broth preparation: Put the enramycin-producing bacteria Streptomyces fungicidicus into the seed tank for seed culture, and connect the seed liquid to the fermenter at a 10% inoculation amount, and the seed culture condition is a temperature of 27 ℃, tank pressure 0.05MPa, aeration ratio v / v =1:1, cycle 29h, rotation speed 120rpm; fermentation conditions: temperature 27℃, tank pressure 0.03MPa, aeration ratio v / v =1:0.8, fermentation cycle 168h , speed 100rpm;

[0033] (2) Inactivation of fermentation broth: the fermentation broth is heated and inactivated for 30 minutes, and after inactivation, the temperature of the fermentation broth is lowered to 40-45 °C, and the temperature for heating and inactivation is 50 °C;

[0034] (3) Pretreatment method: first add chitosan solution with a mass concentration of 0.5% to the ferme...

Embodiment 2

[0039] A method for improving the stability of the enramycin premix finished product is achieved through the following measures:

[0040] (1) Fermentation broth preparation: Put the enramycin-producing bacteria Streptomyces fungicidicus into the seed tank for seed culture, and connect the seed liquid to the fermenter at a 10% inoculation amount, and the seed culture condition is a temperature of 28 ℃, tank pressure 0.05MPa, aeration ratio v / v =1:1, cycle 28h, rotation speed 120rpm; fermentation conditions: temperature 28°C, tank pressure 0.05MPa, aeration ratio v / v =1:0.90, fermentation cycle 192h , speed 120rpm;

[0041] (2) Fermentation broth inactivation: The fermentation broth is heated and inactivated for 30 minutes, and after inactivation, the temperature of the fermentation broth is lowered to 42°C, and the temperature for inactivation is 70°C;

[0042] (3) Pretreatment method: first add chitosan solution with a mass concentration of 0.5% to the fermented liquid after ...

Embodiment 3

[0046] A method for improving the stability of the enramycin premix finished product is achieved through the following measures:

[0047] (1) Fermentation broth preparation: Put the enramycin-producing bacteria Streptomyces fungicidicus into the seed tank for seed culture, and connect the seed liquid to the fermenter at a 10% inoculation amount, and the seed culture condition is a temperature of 28 ℃, tank pressure 0.05MPa, aeration ratio v / v =1:1, cycle 28h, rotation speed 120rpm; fermentation conditions: temperature 30°C, tank pressure 0.06MPa, aeration ratio v / v =1:1, fermentation cycle 216h , speed 140rpm;

[0048] (2) Fermentation broth inactivation: The fermentation broth is heated and inactivated for 30 minutes. After inactivation, the temperature of the fermentation broth is lowered to 40-45 ° C, and the temperature for heating and inactivation is 90 ° C;

[0049] (3) Pretreatment method: first add chitosan solution with a mass concentration of 0.5% to the fermented l...

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Abstract

The invention discloses a method of improving stability of a finished enramycin premixing agent. The method comprises the steps: after the fermentation cultivation of enramycin is finished, inactivating fermentation broth at high temperature, cooling and respectively performing pre-treatment according to chitosan solution and sodium polyacrylate solution; and performing plate-frame filtering and spray drying to the processed fermentation broth to obtain the finished premixing agent. Compared with the present enramycin production technology, the fermentation broth is pre-treated and the drying manner is adjusted, so that the quality of the finished enramycin is changed. The method of improving stability of the finished enramycin premixing agent is strong in operability and low in cost; compared with the non-pretreated finished products or the finished products pre-treated in the other manners, the finished enramycin premixing agent obtained by the method provided by the invention is non-degradable basically and the stability of the finished product is increased during storage. The stability problems of the finished enramycin premixing agent during transportation, storage and usage are solved.

Description

technical field [0001] The invention relates to a method for improving the stability of an enramycin premix finished product, which belongs to the fields of industrial biotechnology and fermentation engineering. Background technique [0002] Enramycin (Enramycin), also known as Anlaimycin, Persistent Mycin, Enramycin, etc., is a polypeptide antibiotic composed of 13 different types of 17 amino acid molecules and fatty acid molecules. The main components are Enramycin Laramycin A (C 107 h 138 C 12 N 26 o 31 ) and enramycin B (C 108 h 140 C 12 N 26 o 31 ) two, in the form of its hydrochloride application. The product was developed by Takeda Pharmaceutical Co., Ltd. in Japan as early as 1966. It was officially registered in Japan in 1974, and has since been registered and widely used in many countries. In 1993, my country approved the registration of enramycin premix, which is used as a drug feed additive. In 2005, the domestic company Zhejiang Hisun Pharmaceutical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/00A61K38/10A61K47/36A61P31/04
Inventor 李国栋邹君彪杜春荣赵明宪邓健刘伯雅
Owner 江西兴鼎科技有限公司
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