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Raoultella ornithinolytica GJ-5 strain and application thereof

A technology of GJ-5 and ornithine, applied in the direction of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve problems such as threats to drinking water safety, eutrophication of water bodies, blooms and red tides, etc., and achieve easy Inactivation, good denitrification effect, simple preparation method

Inactive Publication Date: 2015-03-25
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Discharge of a large amount of ammonia nitrogen wastewater into the water body will easily lead to eutrophication of the water body, cause algal blooms and red tides, and threaten drinking water safety

Method used

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  • Raoultella ornithinolytica GJ-5 strain and application thereof
  • Raoultella ornithinolytica GJ-5 strain and application thereof
  • Raoultella ornithinolytica GJ-5 strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Isolation, screening and identification of high-efficiency nitrosating bacteria:

[0029] (1) Add 5 mL of the activated sludge supernatant to 100 mL of nitrosative bacteria enrichment medium after sterilization at 121°C for 30 minutes, -1 Shaking culture in a constant temperature shaker for 24 h.

[0030] (2) Use Griess reagent to detect whether there is nitrite in the above medium, if it is red, it means that nitrite is formed. Then take 2 mL of culture solution from the above medium, add it into fresh nitrosation-enriched medium, continue to cultivate according to the above conditions and detect whether there is nitrite generation, and repeat this process 3 times.

[0031] (3) Take the bacterial liquid after three cultures, transfer it to the solid separation medium by the dilution plate coating method, place it in an incubator at 30°C for constant temperature cultivation, and select a single colony for purification after 2 days.

[0032] The solid separation medium...

Embodiment 2

[0036] Optimum conditions for efficient nitrosating bacteria:

[0037] Pick a plate to isolate a single colony and inoculate it in LB medium, 30°C, 150r min -1Shaking culture in a constant temperature shaker for 24 h. (1) Take 2 mL of the above-mentioned medium and add it to the medium with different initial ammonia nitrogen concentration (other components of the medium are the same as the enrichment medium for nitrosating bacteria), at 30°C, 150r min -1 Shake culture in a constant temperature shaker for 24 hours, and measure the concentration of ammonia nitrogen and OD in the medium 600 value. When NH 4 -N concentration lower than 100mg·L -1 When the ammonia nitrogen removal effect is higher than 80%. Especially, when the concentration of ammonia nitrogen is 50mg·L -1 , the removal rate of ammonia nitrogen reaches 100%. However, with the increase of the initial ammonia nitrogen concentration, the degradation effect of the bacteria on ammonia nitrogen showed a downward ...

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Abstract

The invention relates to a raoultella ornithinolytica GJ-5 strain and an application thereof. The strain has a collection number of CGMCC NO. 9837; and a 16SrDNA sequence is shown in SEQ ID NO. 1. The raoultella ornithinolytica is streaked on a solid isolation culture medium and is cultured for 24 hours at 30 DEG C; a single colony is inoculated into a 100mL LB culture medium and is cultured for 24 hours in a 150r.min<-1> constant-temperature shaker at 30 DEG C; the single colony is then inoculated into liquid culture media under different conditions and is cultured for 24 hours in a 150r.min<-1> constant-temperature shaker at 30 DEG C, and then the removal efficiency of ammonia nitrogen and the OD600 value are measured. According to the strain, when the ratio of C to N is 6, the removal efficiency of ammonia nitrogen is 52.8% and the strain has good nitrosation and denitrification effects under aerobic condition and is easily inactivated.

Description

technical field [0001] The invention belongs to the field of bacterial strains and applications thereof, and in particular relates to a Raoultella ornithinolyticum GJ-5 bacterial strain and applications thereof. Background technique [0002] The problem of eutrophication in my country's water bodies is becoming more and more serious, and the discharge standards for nitrogen pollutants in water bodies are becoming more and more stringent. In 2012, the Ministry of Environmental Protection raised the sewage discharge standards of industries with large ammonia nitrogen wastewater discharge and high ammonia nitrogen content, such as: synthetic ammonia industry, textile dyeing and finishing industry, coking industry, livestock and poultry breeding industry, etc., especially The requirements for ammonia nitrogen and total nitrogen are becoming more and more stringent. The discharge of a large amount of ammonia nitrogen wastewater into the water body will easily lead to eutrophicat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/01
CPCC02F3/02C02F3/34C12N1/20C12N1/205C12R2001/01Y02W10/10
Inventor 赵晓祥龚娟张弛陈淑丽
Owner DONGHUA UNIV
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