Preparation method of petroleum-degrading acinetobacter junii bacillus

A technique of Acinetobacter awnii and oil degradation, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of inconvenient transportation and storage, large human injury, and high cost, and reduce the cost of oil degradation , no secondary pollution, low price effect

Inactive Publication Date: 2015-03-25
DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In order to solve the problems of inconvenient transportation and storage, great harm to people, and high cost of traditional microbial agents for directly using microbial fermentation liquid to degrade petroleum, the present invention provides a preparation method for oil-degrading Acinetobacter awnii inoculum

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  • Preparation method of petroleum-degrading acinetobacter junii bacillus
  • Preparation method of petroleum-degrading acinetobacter junii bacillus

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specific Embodiment approach 1

[0012] Specific embodiment one: the preparation method of the petroleum degrading Acinetobacter angnei bacterial agent of this embodiment, carries out according to the following steps:

[0013] 1. Pick Acinetobacter junii (Acinetobacter junii) Z3BS and inoculate it into 25mL beef extract peptone medium, cultivate it at 120r / min, 28°C for 16-24h, and obtain the first-grade seed liquid;

[0014] 2. Add the primary seed liquid obtained in step 1 into the fermentation medium at an inoculum size of 0.5% to 2.0% by volume, and cultivate it at 120 to 150 r / min at 28° C. for 24 to 48 hours to obtain a bacterial fermentation liquid;

[0015] 3. After sterilizing corn cob powder, rice bran and wheat bran, take any two of corn cob powder, rice bran and wheat bran and mix them at a weight ratio of 1:1 to obtain a mixture. Add bacterial fermentation broth, chlorine Sodium chloride and potassium dihydrogen phosphate are mixed evenly and then dried and sealed at 28°C for storage to obtain th...

specific Embodiment approach 2

[0020] Embodiment 2: This embodiment differs from Embodiment 1 in that: the first-grade seed liquid obtained in Step 1 is added to the fermentation medium at an inoculum volume of 1% to 1.5% by volume. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0021] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the formula of fermentation medium in step two is: choose any two from corn cob, rice bran, wheat bran, each kind of material is 5g / L, sodium chloride 2g / L, potassium dihydrogen phosphate 1g / L, ammonium nitrate 1g / L, anhydrous magnesium sulfate 0.05g / L, anhydrous calcium chloride 0.05g / L, add distilled water to 1L, pH is 7.2. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses a preparation method of petroleum-degrading acinetobacter junii bacillus, relates to a preparation method of a petroleum-degrading bacterial agent, and is used for solving the problems that transportation and storage are inconvenient due to the fact that the petroleum is directly degraded by using the bacterial fermentation liquor, the injuries to human bodies are relatively large, and a traditional microbial agent is low in cost. The method comprises the following steps: 1, selecting acinetobacter junii, inoculating into a culture medium, and cultivating to obtain a first-stage seed solution; 2, adding the first-stage seed solution into a fermentation culture medium, and cultivating to obtain a bacterial fermentation liquor; and 3, sterilizing corncob powder, rice bran and wheat bran, mixing any two of the corncob powder, the rice bran and the wheat bran, adding the bacterial fermentation liquor, sodium chloride and monopotassium phosphate to the mixture, mixing evenly, baking, and hermetically storing, so as to obtain the petroleum-degrading acinetobacter junii bacillus. The petroleum-degrading acinetobacter junii bacillus has the advantages of being low in price, easy to degrade, and free of secondary pollution; meanwhile, the soil fertility can be improved; and the method is applied to degradation of petroleum.

Description

technical field [0001] The invention relates to a preparation method of a petroleum degrading bacterial agent. Background technique [0002] Petroleum, also known as crude oil, has very complex components and is extremely difficult to degrade. In recent years, with the increase of petroleum exploitation and usage, the degradation of petroleum pollutants has been paid more and more attention. Microbial degradation has become a research hotspot because of its low cost, high efficiency, and no secondary pollution. However, there are many problems in directly using microbial fermentation broth to degrade petroleum. First of all, the volume of fermentation broth is relatively large, and if it is directly applied, it will bring great inconvenience to transportation and storage. Moreover, the heavy odor of the general microbial fermentation liquid is more harmful to the staff, which does not conform to the concept of "people-oriented". Therefore, many sciences have proposed met...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCC12N1/20B09C1/10
Inventor 杨庆丽董艳姬妍茹刘宇峰张正海高媛王月明潘静
Owner DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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