Method for rapid analysis of flavonoid glycoside and phenolic acid active ingredients in ixeris sonchifolia injection by virtue of high performance liquid chromatography (HPLC)
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A rapid analysis technology for flavonoid glycosides and phenolic acids, which is applied in the fields of analyzing materials, separating materials, measuring devices, etc.
Active Publication Date: 2015-03-25
TONGHUA HUAXIA PHARMA
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[0003] Aiming at the defects of the existing Kudiezi injection analysis method, the present invention provides a method for rapidly analyzing the active components of flavonoid glycosides and phenolic acids in Kudiezi injection by using HPLC
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Embodiment 1
[0015] Method for rapid analysis of flavonoid glycosides and phenolic acids in Kudiezi injection by HPLC:
[0019] Chromatographic column: UltimateTMAQ-C18 (4.6×250 mm, 5 μm) (Welch Materials Company); mobile phase: A phase organic phase, B phase is 1% (v / v) formic acid aqueous solution; flow rate is 1 mL min-1 ; The detection wavelength is 348 nm; The injection volume is 10 μL.
[0020] Mobile phase: Phase A is a mixed solution of tetrahydrofuran and methano...
Embodiment 2
[0040] Method for rapid analysis of flavonoid glycosides and phenolic acids in Kudiezi injection by HPLC:
[0041] The conditions within the optimized range of chromatographic analysis were selected for the analysis experiment of Kudiezi injection. (1) Instruments and materials. Shimadzu LC-2010A high performance liquid chromatography (SHIMADZU company), UV detector, UltimateTMAQ-C18 column (4.6 × 250 mm, 5 μm, Welch Materials company). Kudiezi injection (Tonghua Huaxia Pharmaceutical Co., Ltd., batch number 91112), chromatographic methanol, chromatographic acetonitrile, chromatographic tetrahydrofuran, chromatographic formic acid (Shandong Yuwang Company), double distilled water (self-made in the laboratory). (2) Chromatographic conditions. Ultimate AQ-C18 chromatographic column (4.6 × 250 mm, 5 μm, Welch Materials Company); column temperature 40 ° C; mobile phase: A phase organic phase, B phase is 1% (v / v) formic acid aqueous solution; flow rate is 1 mL ·min-1; the detect...
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Abstract
The invention belongs to the field of analyzing Chinese herbal medicinal ingredients, and in particular discloses a method for rapid analysis of flavonoid glycoside and phenolic acid active ingredients in an ixeris sonchifolia injection by virtue of high performance liquid chromatography (HPLC). Chromatographic analysis conditions are as follows: (1) chromatographic column is a C18 reversed-phase chromatographic column; (2) a mobile phase consists of methanol, water, tetrahydrofuran and acid, and volume ratios of various ingredients in the mobile phases are as follows: the ratio of the tetrahydrofuran to the methanol is 0.03-0.1, the total proportion of the tetrahydrofuran and the methanol is 30-40% and the proportion of the acid is 0.1-2% of the water phase; (3) an ultraviolet detection wavelength is 348nm; and (4) chromatographic column temperature is 35-45 DEG C. Under an isocratic elution condition, more than 15 chromatographic peaks can be simultaneously separated and analyzed within 20min; compared with former methods, the method disclosed by the invention can be used for solving a problem that luteolin-7-O-beta-D-glucoside and luteolin-7-O-beta-D-glucuronide are difficult to separate, and the phenolic acid chromatographic peak in the ixeris sonchifolia injection can be better separated; and furthermore, analysis time is greatly shortened by the method.
Description
technical field [0001] The invention belongs to the field of traditional Chinese medicine component analysis. That is, a method for rapid analysis of flavonoid glycosides and phenolic acids in Kudiezi injection by HPLC. Background technique [0002] Kudiezi Injection is an intravenous injection made by extracting and refining the herb of Compositae, Kudiezi, and its main components include phenolic acids and flavonoids. Kudiezi injection is included in the national drug standard product (Z20025450), produced by Tonghua Huaxia Pharmaceutical Co., Ltd. It is widely used in the treatment of cardiovascular and cerebrovascular diseases because of its functions of promoting blood circulation, relieving pain, clearing heat and removing blood stasis. Long-term clinical practice has proved that it is safe to use and has definite curative effect. Luteolin-7-O- β -D glucuronide belongs to flavonoid glycosides, and its content in Kudiezi medicinal material is high and relatively stab...
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