Salmonella immunochromatography test strip based on low-noise excitation type fluorescent mark

An immunochromatographic test strip, Salmonella technology, applied in material excitation analysis, fluorescence/phosphorescence, analysis materials, etc., can solve the problem of difficult and rapid quantitative detection of Salmonella, and achieve the effect of high signal-to-noise ratio, improved sensitivity, and high detection sensitivity

Inactive Publication Date: 2015-03-25
BEIJING ENTRY EXIT INSPECTION & QUARANTINE BUREAU INSPECTION & QUARANTINE TECH CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] For this reason, the technical problem to be solved by the present invention lies in the problem that it is difficult to quickly and quantitatively detect Salmonella in the prior art, and then provide a kind of Salmonella immunochromatographic test strip based on low-noise excitation fluorescent labeling, and further disclose its application

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  • Salmonella immunochromatography test strip based on low-noise excitation type fluorescent mark
  • Salmonella immunochromatography test strip based on low-noise excitation type fluorescent mark
  • Salmonella immunochromatography test strip based on low-noise excitation type fluorescent mark

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Embodiment 1

[0042] The Salmonella immunochromatographic test strip based on low-noise excitation fluorescent labeling described in this embodiment is as follows: figure 1 As shown, it includes a bottom plate 7 and a sample pad 1, a binding pad 2, an antibody-carrying membrane 3 and a water-absorbent pad 6 that are sequentially adhered to the bottom plate along the length direction of the bottom plate 7, and the sample pad 1 , binding pad 2, antibody-carrying membrane 3 and water-absorbing pad 6, which are sequentially in contact with and only partially overlap adjacent parts; the antibody-carrying membrane 3 is adhered to the middle part of the bottom plate 7, and A quality control line 5 with spaced detection lines 4 is provided, the detection line 4 is close to the binding pad 2 , and the quality control line 5 is close to the water-absorbing pad 6 . The quality control line 4 is arranged parallel to the detection line 5, and the distance between the detection line and the quality contr...

Embodiment 2

[0052] This embodiment provides a method for qualitatively detecting Salmonella using the above-mentioned Salmonella immunochromatographic test strip based on low-noise excitation fluorescent labeling, comprising the following steps:

[0053] a) Take 25g of chicken sample, cut it into pieces, dilute the sample with 225mL of PBS buffer solution containing 5%BSA and 0.1%Tween20 with a pH of 7.4, take the supernatant as a test sample, draw 1ml of the supernatant into the EP tube, Ultrasonic bacteria destruction, probe with a diameter of 3mm, work for 5s, rest for 15s, 40% energy (ultrasonic cell pulverizer provided by Ningbo Xianchang Electronic Technology Co., Ltd., model XC-CD), a total of 30 cycles, absorb 100μL by ultrasonic Add the treated sample dropwise to the sample pad 1, and then add 50 μL of the above-mentioned PBS buffer dropwise after the absorption is dry, and perform immunochromatography with the test strip;

[0054] b) After standing at room temperature for 15 min...

Embodiment 3

[0057] This embodiment provides a method for the quantitative detection of Salmonella using the low-noise excitation-based fluorescent-labeled Salmonella immunochromatographic test strip.

[0058] Preparation of standard curve: take 10 5 The Salmonella pure bacterium (laboratory isolate) of CFU / mL, carries out multiple serial dilution to described pure bacterium with containing PBS (PH7.4) diluent, makes 0.5*10 5 CFU / mL, 1*10 4 CFU / mL, 0.5*10 4 CFU / mL, 1*10 3 CFU / mL, 0.5*10 3 CFU / mL, 1*10 2 CFU / mL, 10CFU / mL samples; then perform ultrasonic treatment according to the method described in step a in Example 2, draw 50 μL of the sample and drop it on the sample pad 1, and then add 50 μL of the above-mentioned PBS dropwise after the sample is absorbed Buffer solution, let stand at room temperature for 10 minutes, put the above test strip into a portable low-noise excitation fluorescence scanner (provided by Beijing Borunfude Technology Development Co., Ltd.) for reading.

[00...

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Abstract

The invention relates to a salmonella immunochromatography test strip based on a low-noise excitation type fluorescent mark and application of the salmonella immunochromatography test strip. A low-noise excitation type fluorescent dye is used as a mark and an immunochromatography technology is adopted to prepare the immunochromatography test strip targeting salmonella; when the salmonella immunochromatography test strip is used for detecting, a special portable type low-noise excitation type fluorescent scanner is used for scanning a quality control line and a detection line respectively to detect a line fluorescence intensity detection value so that the qualitative and quantitative detection of a sample is realized; and the test strip is applicable to instant detection of salmonella in foods and pathological samples. The salmonella immunochromatography test strip has the characteristics of rapidness, high sensitivity, qualitativeness and accurate quantitativeness and portability.

Description

technical field [0001] The invention belongs to the field of food detection, and in particular relates to a Salmonella immunochromatographic test strip based on a low-noise excitation type fluorescent dye label and a preparation method and application thereof. Background technique [0002] As an important indicator of pathogenic bacteria detection, Salmonella is of great significance in public health, food hygiene, animal husbandry and veterinary medicine, and entry-exit inspection and quarantine. Require. In order to seek fast, accurate, high-sensitivity, easy-to-operate and quantitative detection methods, scholars from all over the world have conducted a lot of research, developing from traditional methods to rapid detection methods based on immunology and molecular biology, and in practice Continuously making new progress. [0003] Immunochromatography technology is a new type of immunoassay technology that effectively combines the excellent separation ability of chroma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/533G01N21/64
CPCG01N33/56916G01N33/533
Inventor 张捷谢雪钦万晓楠陈广全张惠媛康西西尚士进张晓光舒咬根
Owner BEIJING ENTRY EXIT INSPECTION & QUARANTINE BUREAU INSPECTION & QUARANTINE TECH CENT
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