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Two-material hollow casing pipe for separating primary respiratory tract epithelial cells

An epithelial cell and dual-material technology, which is applied in the field of medical instruments, can solve the problems of loss of cilia directional swing in vivo physiological functions, expensive respiratory epithelial cells, and difficult clamping of reentrant arterial clips. The effect of strong repeatability and easy promotion and application

Inactive Publication Date: 2015-04-01
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are following problems in the operation process of this method: if the selected material is too strong and less elastic, the needle will not be inserted tightly, and it will be difficult to turn back and hold the arterial clip, which will easily cause pronase leakage; The strength of the material is small but the elasticity is high, which will cause the surgical thread to be tied tightly when it is ligated, and it will also cause pronase leakage, etc.
[0006] At present, the commercially available primary airway epithelial cells are expensive, and most of them have lost the in vivo physiological function of cilia directional swing, and their activity is poor

Method used

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  • Two-material hollow casing pipe for separating primary respiratory tract epithelial cells
  • Two-material hollow casing pipe for separating primary respiratory tract epithelial cells
  • Two-material hollow casing pipe for separating primary respiratory tract epithelial cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1. The making of two-material bushing

[0029] (1) Take a GA-1073 silicone tube with 45-degree inclined ends and flat ends at both ends, with an inner diameter of 0.6mm and an outer diameter of 0.8mm;

[0030] (2) Take 25mm Teflon hollow tube with 20mm and both ends are flat ends, inner diameter 0.8mm, outer diameter 1.0mm,

[0031] (3) Apply 10 microliters of Wenjin Chaoneng 380 silicone glue to the flat end of the GA-1073 silicone tube, insert it into the Teflon hollow tube, insert 5mm and fix it for 15 seconds to obtain the dual-material sleeve.

[0032] figure 1 Shown is a perspective view of the dual-material hollow sleeve of the present invention (taking the special dual-material hollow sleeve for mice as an example), wherein

[0033] 1 is Teflon hollow tube, length: 25mm

[0034] 2 is GA-1073 silicone tube, length: 20mm

[0035] BB' is Teflon hollow tube outer diameter: 1.0mm

[0036] CC' is a GA-1073 silicone tube inserted into a Teflon hollow tu...

Embodiment 2

[0039] Example 2. Isolation of mouse trachea

[0040] (1) BALB / c mice were sacrificed by neck dislocation, their heads were tilted up, and their chest and abdomen were soaked in alcohol for 30 seconds for disinfection, taking care to protect the oral cavity and nasal passages from alcohol stimulation. Fix it on the operating table, cut the skin along the linea alba under aseptic conditions, expose the abdominal aorta, incise and let the blood flow out, the purpose is to avoid mixing too many red blood cells when separating the respiratory epithelial cells;

[0041] (2) Bluntly dissect the trachea, expose the trachea, and fully dissociate it;

[0042] (3) Wear the double-strand No. 1 surgical thread 100mm, cut a small gap 5mm below the cricoid cartilage of the trachea, insert the 45-degree oblique end of the dual-material sleeve GA-1073 silicone tube, insert half of the trachea to a depth of about 5mm, and use No. 1 surgical thread Tie a tight knot on the outside of the trache...

Embodiment 3

[0045] Example 3. Digestion of Tracheal Tissue and Removal of Red Blood Cells

[0046] (1) Inject a little 0.05% pronase pre-cooled at 4°C into the trachea fixed at the tip of the cannula through the dual-material cannula, and wash the inner wall of the trachea to remove the washed PBS;

[0047] (2) Fill the entire trachea with 0.05% pronase, so that the trachea expands slightly like a balloon, and clamp the half-folded Teflon hollow tube with a 30mm arterial clamp to seal the entire trachea-cannula combination;

[0048] (3) Fix and infiltrate the entire trachea in MEM added with penicillin, and digest for 8 hours;

[0049] (4) Cut off the lower trachea, collect the digestive juice, and use a 1ml syringe to draw PBS to flush the trachea;

[0050] (5) Collect about 1.5ml of washing liquid (collecting liquid + PBS washing trachea liquid), transfer it to an EP tube after merging, and centrifuge at 500X g for 4 minutes to remove the supernatant;

[0051] (6) Add 150μl of erythro...

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Abstract

The invention relates to a tool for in-vivo separating primary respiratory tract epithelial cells, and particularly relates to a two-material hollow casing pipe for separating primary respiratory tract epithelial cells. The two-material hollow casing pipe is characterized by being formed by bonding a silicone tube (small pipe diameter and high strength) and a Teflon hollow pipe (large pipe diameter, small strength and large flexibility) by virtue of silicone glue.

Description

technical field [0001] The invention relates to the field of medical instruments, in particular to a dual-material hollow sleeve for separating primary respiratory epithelial cells. Background technique [0002] Respiratory epithelial cells are the first natural barrier between the respiratory system and the outside world, and their structural and functional homeostasis is the cellular basis for the respiratory system to maintain normal ventilation and defense functions. Respiratory epithelial cells attach to the basement membrane and grow together to form the pseudostratified columnar ciliated epithelium of the respiratory tract. The cilia on its surface swing rhythmically to the pharynx, which plays an important role in the process of eliminating irritating foreign bodies in the respiratory tract. [0003] The primary airway epithelial cells of experimental animals, as the experimental model of human airway epithelial cells, have an irreplaceable reference for clinical and...

Claims

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Application Information

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IPC IPC(8): A61B10/02
Inventor 李煜生任杰
Owner SOUTHERN MEDICAL UNIVERSITY
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