The invention discloses an orientation positioning seeder. The orientation positioning seeder comprises a workbench, a conveying device, an aperture disk, an aperture pressing mechanism, an oriented seed aperture device, a negative pressure seed discharge device, a sprinkling device, a guide rod, a position sensor and a control case. The workbench comprises a frame and a workbench surface. The conveying device is installed on the workbench surface and is used for conveying the aperture disk according to a flow line working process so that the aperture disk goes through the aperture pressing mechanism, the oriented seed aperture device, the negative pressure seed discharge device and the sprinkling device, PLC is used for controlling the whole seeder, the oriented seed aperture device adopting a machine vision technology realizes seed direction adjustment, the negative pressure seed discharge device carries out orientation positioning sowing on the seeds subjected to direction adjustment, the sprinkling device is used for watering the apertures with the seeds, and the position sensor is used for accurately detecting and feeding back the work process. The orientation positioning seeder realizes 45 degree orientation positioning sowing of large seeds. The orientation positioning seeder solves the problem that the manual sowing method has low work efficiency and causes fatigue easily, can cultivate standard seedlings and provides favorable conditions for automatic grafting implementation.