Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of Alternaria y1309-1 and its application

A technology of Alternaria spp. and thalli cells is applied to Alternaria spp. Y1309-1 and its application field, which can solve the problems of high energy consumption, high equipment requirements, chemical pollution, etc., and achieve the effect of good uniformity

Active Publication Date: 2017-09-19
SOUTHEAST UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The physical method requires high equipment, expensive production costs, and high energy consumption
The chemicals used in the chemical method will pollute the environment, and will also be adsorbed on the surface of nano-silver and affect its application in the field of medicine; and the nano-silver produced by it will aggregate over time, so storage will affect the quality of nano-silver. particle size

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of Alternaria y1309-1 and its application
  • A kind of Alternaria y1309-1 and its application
  • A kind of Alternaria y1309-1 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: the culture condition of Alternaria Y1309-1

[0039] Alternaria Y1309-1 was cultured on PDA solid medium (potato 20%, glucose 2%, deionized water 1000mL, natural pH, sterilized at 121°C for 30min before use) at 28°C, and on liquid medium (PDA solid Culture medium: 20% potato, 2% glucose, 1.5% agar, 1000mL deionized water, natural pH, sterilized at 121°C for 30min and cultured at 28°C and 150rpm.

Embodiment 2

[0040] Example 2 Extracellular preparation of nano-silver by Alternaria Y1309-1

[0041] Step 1 Alternaria was cultured on PDA solid medium at 28°C for 4 days;

[0042] Step 2 Inoculate Alternaria in 100mL potato dextrose liquid medium and culture at 28°C and 150rpm for 2 days;

[0043] Step 3 Collect the bacteria and wash with sterile water, add 20g of wet bacteria into 100ml of sterile deionized water, and incubate at 28°C, pH 7, 150rpm for 48h;

[0044] Step 4 removes the thalline and collects the supernatant;

[0045] Step 5 Add silver nitrate solution (final concentration 0.5-2.0 mmol / L) to the above supernatant, and react at 28° C. and 150 rpm for 12-48 hours to obtain silver nanoparticles with a particle size of 40-50 nm.

[0046] attached figure 1 The left is the supernatant before adding silver nitrate, and the right is the dark brown nano-silver solution after adding silver nitrate to react for 48 hours. attached image 3 The ultraviolet-visible spectrogram of ...

Embodiment 3

[0047] Example 3 Microbial Alternaria Y1309-1 intracellular preparation of nano-silver

[0048] In step 1, Alternaria was cultivated at 28° C. for 4 days on the PDA solid medium;

[0049] Step 2 inoculate Alternaria in 100mL potato dextrose liquid medium, and cultivate it at 28°C and 150rpm for 2 days;

[0050] Step 3 Collect the bacteria, wash with sterile water, add 20g of wet bacteria to 100ml of sterile silver nitrate solution (final concentration 0.5-2.0 mmol / L), react at 28°C, pH 7, 150rpm for 6-48h, Intracellular preparation of nano-silver;

[0051] Step 4: Destroy the thalline and collect the nano-silver particles.

[0052] attached figure 2 The left is the bacteria before adding silver nitrate, and the right is the bacteria coated with nano-silver after adding silver nitrate for 48 hours, which is yellowish brown.

[0053] attached Image 6 It is a transmission electron micrograph of silver nanoparticles prepared in Alternaria cells, showing that the silver nano...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses Alternaria Y1309-1. The method for preparing nano-silver has mild conditions, does not need to use a variety of chemical reagents, has low reaction cost and is environmentally friendly. The invention also discloses the application of Alternaria Y1309‑1 in the preparation of metal nanomaterials. The invention also discloses the application of Alternaria Y1309‑1 in preparing nano silver. The nano-silver particles prepared by the invention have good uniformity, can be independently dispersed in water, and are stable at normal temperature. The nano-silver produced by the invention has certain inhibitory activity on Escherichia coli and Staphylococcus aureus. The invention can be applied in the fields of medicine, catalysis, environment restoration and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to Alternaria Y1309-1 and its application. Background technique [0002] Nano-silver has unique thermal, optical, electrical, magnetic, catalytic and sensitive properties, and is widely used in catalyst materials, antistatic materials, low-temperature superconducting materials, electronic pastes, biosensor materials, and biomedicine. [0003] At present, there are physical, chemical and biological methods for the synthesis of nano-silver. The physical method requires high equipment, high production costs, and high energy consumption. The chemicals used in the chemical method will pollute the environment, and will also be adsorbed on the surface of nano-silver and affect its application in the field of medicine; and the nano-silver produced by it will aggregate over time, so storage will affect the quality of nano-silver. particle size. Biosynthesis method has become an important bran...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P3/00C12R1/645
CPCC12P3/00C12N1/145C12R2001/645
Inventor 陈峻青李黎吉民戚文秀
Owner SOUTHEAST UNIV