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Silver carp dpp-iv inhibitory polypeptide and its application

A DPP-IV, silver carp technology, applied in the field of medicine, can solve problems such as no relevant research on DPP-IV inhibitory active peptides, and achieve good DPP-IV inhibitory activity, beneficial to human body utilization, and high DPP-IV inhibitory activity. Effect

Inactive Publication Date: 2019-04-26
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, there is no relevant research on the preparation of DPP-IV inhibitory active peptides by using silver carp as raw material through enzymatic hydrolysis.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: the preparation of silver carp meat protein solution

[0042] After the silver carp is killed, remove the scales and viscera, wash and collect the meat, select the white meat on the back of the silver carp, cut it into small pieces and use a grinder to stir it into minced meat; then mix the minced silver carp with deionized water, adjust The protein concentration is 5%, and the fish meat turbid liquid with adjusted protein concentration is placed in a 95°C water bath for 10 minutes to kill the enzyme, so as to eliminate the endogenous protease in the fish meat; after cooling, carry out homogenization treatment to obtain silver carp fish meat protein solution, freeze-dried for future use or directly proceed to the enzymatic hydrolysis reaction in the next step.

Embodiment 2

[0043] Embodiment 2: Preparation of freshwater fish silver carp polypeptide

[0044] The first step of enzymatic hydrolysis reaction: take a certain amount of fish protein powder and dissolve it in distilled water (5%, w / w). Place the solution in a constant temperature water bath at 37°C. When the temperature of the solution rises to 37°C, adjust its pH value to 2.0 with 1mol / L HCl, and add pepsin to the solution to start the first step of enzymatic hydrolysis reaction. The weight ratio of the substance, that is, E:S is 1%, and after hydrolysis at 37° C. for 1 hour, the hydrolyzate of pepsin is obtained;

[0045] The second step of enzymatic hydrolysis reaction: use 0.9mol / L NaHCO 3 Adjust the pH value of the hydrolyzate of pepsin to 5.3, then adjust its pH value to 7.5 with 1mol / L NaOH, then add trypsin, the weight ratio of enzyme and substrate, that is, E:S is 2%, start the second step Enzymolysis reaction, after 2 hours of reaction at 37°C, the enzymatic hydrolyzate of tr...

experiment example 3

[0048] Experimental Example 3: Optimization of Enzymolysis Reaction Conditions

[0049] Using the method of Example 2, the enzymatic hydrolyzate and reaction conditions of the enzymatic hydrolysis reaction were optimized, and the DPP-IV inhibitory activity in the final product was detected. The results are shown in Table 1:

[0050] Table 1: Enzymolysis reaction conditions and the degree of hydrolysis and DPP-Ⅳ inhibitory activity during the enzymolysis process

[0051] Protease type

Substrate concentration

pH

temperature

Enzyme / substrate ratio

Enzymolysis time

degree of hydrolysis

DPP-Ⅳ inhibition rate

Before enzymatic digestion

/

/

/

/

0h

0

7.74％

Pepsin

5％

2.0

37℃

1％

1h

7.88％

40.57％

trypsin

5％

8.0

37℃

2％

3h

20.26％

47.98％

Dispase

5％

7.5

45℃

3％

3.5h

22.71％

49.34％

Dispase

5％

7.5

45℃

...

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PUM

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Abstract

The invention relates to a silver carp DPP-IV inhibitory polypeptide and applications thereof. The inhibitory polypeptide is a natural product by fine hydrolysis of fish protein of freshwater fish silver carp by protease, namely a silver carp protease functional polypeptide. The DPP-IV inhibitory polypeptide prepared by subjecting the silver carp protein to three-section enzymolysis by pepsin, pancreatin and neutral protease has good DPP-IV inhibition activity, has an inhibition rate reaching 51.59% (with the polypeptide concentration being 5 mg / mL), and has good tolerant gastrointestinal digestion stability. The DPP-IV inhibitory polypeptide is characterized by being natural, safe, efficient, and low in cost, is a prospect food functional base material for diabetes prevention or treatment, and can be used for preparation of medicines or functional foods for prevention or auxiliary treatment of diseases benefited from DPP-IV inhibition.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a freshwater fish silver carp polypeptide with DPP-IV inhibitory activity and application thereof. Background technique [0002] Diabetes mellitus (DM) is one of the diseases with the fastest growing rate and the most widespread incidence in the world, and has become the "third killer" after tumors and cardiovascular diseases. Diabetes mellitus is a group of chronic metabolic abnormal syndromes caused by the interaction of multiple genetic and environmental factors. It is caused by the relative or absolute lack of insulin and the disturbance of carbohydrate, protein and fat metabolism caused by the surrounding target tissues to different degrees of insulin resistance. Yes, it can lead to blindness, cardiovascular and cerebrovascular diseases, renal failure, neuropathy, limb gangrene leading to amputation, coma and other complications, seriously endangering human health. Type 2 ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/18A61K38/01A61P3/10A61P37/04A61P9/00A23L33/18A23L33/00
Inventor 陈尚武张颖
Owner CHINA AGRI UNIV
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