High-yield gamma-aminobutyric acid lactobacillus and screening method thereof

A technology of aminobutyric acid lactic acid bacteria and aminobutyric acid, which is applied in the biological field, can solve the problems of reducing product production cost input, and achieve the effect of reducing production cost input

Inactive Publication Date: 2015-04-01
LINYI GERUI FOOD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The invention solves the problem that traditional food is added with gamma-aminobutyric ac...

Method used

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  • High-yield gamma-aminobutyric acid lactobacillus and screening method thereof
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  • High-yield gamma-aminobutyric acid lactobacillus and screening method thereof

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Embodiment Construction

[0041] One, the culture medium used in the invention

[0042]MRS medium: peptone 1%, beef extract 1%, yeast extract 0.5%, glucose 0.5%, sodium acetate 0.5%, diamine citrate 0.2%, Tween 80 0.1%, dipotassium hydrogen phosphate 0.2%, Magnesium sulfate 0.02%, manganese sulfate 0.005%, calcium carbonate 2%, distilled water 1000 mL, pH 6.5.

[0043] GYP fermentation medium: glucose 1%, yeast extract 1%, peptone 0.5%, sodium acetate 0.2%, magnesium sulfate 0.02%, manganese sulfate 0.01%, ferrous sulfate 0.01%, sodium chloride 0.01%, L-glutamic acid 1%.

[0044] Concrete steps of the γ-aminobutyric acid-containing fermented milk process are as follows:

[0045] 2. Screening of lactic acid bacteria producing gamma-aminobutyric acid

[0046] The scope of screening for lactic acid bacteria producing GABA is the strains used by the company to produce yogurt. The screening process is as follows:

[0047] 1. Fermentation culture of lactic acid bacteria

[0048] First, use MRS medium fo...

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Abstract

The invention discloses a high-yield gamma-aminobutyric acid lactobacillus and a screening method thereof, belonging to the field of biotechnology. The high-yield gamma-aminobutyric acid lactobacillus is prepared by the following steps: 1) fermentation culture of lactobacillus for test; 2) preliminary screening of gamma-aminobutyric acid producing bacteria according to whether the lactobacillus has glutamate decarboxylase activity; and 3) secondary screening of high-yield gamma-aminobutyric acid lactobacillus. After screening with the method, the content of gamma-aminobutyric acid in the fermentation liquid after the fermentation culture reaches 5.025g/L, and the conversion rate of L-sodium glutamate reaches 90.05% of lactobacillus plantarum. The method disclosed by the invention can be used for solving the problem of exogenously adding gamma-aminobutyric acid or extremely low content of gamma-aminobutyric acid in traditional food, and reducing the production cost of the products.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a screening method for a high-yielding gamma-aminobutyric acid lactic acid bacterium. Background technique [0002] γ-aminobutyric acid, chemical name: 4-aminobutyric acid, English name: γ-aminobutyric acid (GABA), structural formula: NH 2 -CH 2 -CH 2 -COOH; molecular formula: C 4 h 9 NO 2 ; Molecular weight: 103.1 (Qu Yan et al. γ-aminobutyric acid and its research progress in soybean fermented food [J]. China Brewing, 2010, 3:1-4) The structural formula of GABA is: [0003] [0004] GABA has an amino group on the γ-position carbon atom, which exists in an unbound state and is very soluble in water. GABA is widely distributed. Studies have found that γ-aminobutyric acid exists in animals, plants and microorganisms. GABA has Lower blood pressure, improve nervous function, regulate body metabolism, regulate heart blood, regulate hormone secretion and other functio...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12N1/20C12R1/25
Inventor 赵迎庆
Owner LINYI GERUI FOOD
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