Biological-compatibility artificial ear and in-vitro rapid construction method thereof

A biocompatible, artificial ear technology, applied in the direction of prosthesis, medical science, etc., can solve the problems of high cost of plastic surgery and low biocompatibility of artificial ear, and achieve the effect of reducing biological toxicity and high repeatability

Inactive Publication Date: 2015-04-08
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Through the above analysis, the biocompatibility of the artificial ear under the existing technical conditions is low, and it only has the effect of surgical plastic surgery, and the cost of plastic surgery is high, and most of them are repair methods based on physics.

Method used

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  • Biological-compatibility artificial ear and in-vitro rapid construction method thereof
  • Biological-compatibility artificial ear and in-vitro rapid construction method thereof
  • Biological-compatibility artificial ear and in-vitro rapid construction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1: A biocompatible artificial ear was fabricated by multi-nozzle three-dimensional printing.

[0066] a) Designing the digital three-dimensional model of the artificial ear by a computer, and assigning a print forming path by the computer, and controlling the operation of the three-dimensional printing device;

[0067] b) Mix the prepared sodium alginate with a mass volume concentration of 2% and 20% gelatin to obtain a natural polymer sodium alginate / gelatin hydrogel, mix the chondrocytes with the hydrogel, and mix the The concentration of chondrocytes was 10 8 each / mL; the hydrogel containing chondrocytes was loaded into the extrusion nozzle assembly of the composite multi-nozzle 3D printing equipment; using the microcapsule preparation process, the hydrogel containing adipose-derived stem cells and endothelial cell growth factor with a particle size of 100 μm was obtained Gel microcapsules, the microcapsules were suspended in phosphate buffered saline (PBS),...

Embodiment 2

[0075] Example 2: A biocompatible artificial ear was prepared by using multi-nozzle three-dimensional printing.

[0076] a) Designing the digital three-dimensional model of the artificial ear by a computer, and assigning a print forming path by the computer, and controlling the operation of the three-dimensional printing device;

[0077] b) Mix the prepared sodium alginate with a mass volume concentration of 3% and 15% gelatin to obtain a natural polymer hydrogel, and combine chondrocytes, adipose stem cells, and Schwann cells with the hydrogel respectively Mix, the mixed chondrocyte density is 10 7 cells / mL, the density of adipose-derived stem cells was 10 4 cells / mL, the Schwann cell density was 10 6 each / mL; the cell-containing hydrogel is loaded into the extrusion nozzle assembly of the composite multi-nozzle 3D printing device; using the microcapsule preparation process, the 1% fibrinogen hydrogel of endothelial cell growth factor with a particle size of 20 μm is obtain...

Embodiment 3

[0084] Example 3: A biocompatible artificial ear embedded with a cochlear implant was fabricated by multi-nozzle three-dimensional printing.

[0085] a) The digital three-dimensional model is obtained by CT scanning the external ear pinna of normal human body, which is used as the contour design of the artificial ear, and the computer assigns the printing forming path to control the operation of the three-dimensional printing equipment;

[0086] b) The prepared fibrinogen with a mass volume concentration of 1% and collagen 5% is used to obtain a natural polymer hydrogel, and the cartilage stem cells are mixed with the hydrogel, and the concentration of the mixed cartilage stem cells is 10 5 each / mL; the hydrogel containing cartilage stem cells was loaded into the extrusion nozzle assembly of the composite multi-nozzle 3D printing equipment; the microcapsule preparation process was used to obtain the hydrogel microcapsules containing nerve cell growth factor with a particle size...

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Abstract

The invention discloses a biological-compatibility artificial ear and an in-vitro rapid construction method thereof, and belongs to the field of biological organ engineering and advanced manufacture. A rapid forming technology is used for constructing an artificial ear in vitro, and the artificial ear has a feature and functions similar with a natural auricle, and can be compounded with an artificial auricle assembly to restore hearing. The artificial ear comprises a skin simulation layer, a hydrogel layer, a hydrogel micro-capsule layer and a skeleton simulation layer, wherein the skin simulation layer and the skeleton simulation layer are made of a synthetic macromolecule material; the hydrogel layer and the hydrogel micro-capsule layer are made of a natural macromolecule material which contains cells and growth factors, have strong bioactivity and are favorable for the regeneration of (cartilage) tissues, blood systems and nervous systems. The biological-compatibility artificial ear also can be compounded with the artificial auricle assembly which comprises an inner part of the auricle and an outer part of the auricle. The biological-compatibility artificial ear can be used for a plastic surgery to replace the malformed auricle of a patient. After the artificial auricle assembly is compounded, the artificial ear can be used for treating and restoring the auditorily handicapped.

Description

technical field [0001] The invention belongs to the field of tissue and organ manufacturing and bioengineering, and relates to a biocompatible artificial ear and a rapid construction method thereof in vitro. Background technique [0002] Treatment of ear injuries and deformities caused by congenital and acquired factors is still a clinical problem. Patients with congenital microtia have incomplete auricle development, accompanied by certain atresia or stenosis of the external auditory canal and deformity of the middle ear, but Nell develops normally and still retains some hearing. Congenital microtia is generally treated by total auricle reconstruction and auditory function enhancement surgery. For patients with abnormal development of the outer ear and inner ear, simultaneous treatment of outer ear plastic surgery and hearing restoration is required. [0003] Congenital microtia and goblet ears are two common congenital ear deformities. The current treatment options mainl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/18A61L27/38A61L27/26A61L27/24A61L27/22A61L27/20A61L27/18A61L27/16A61L27/52A61L27/56A61L27/54
Inventor 王小红许雨帆
Owner TSINGHUA UNIV
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