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Preparation method of adsorbent for adsorbing low-density lipoprotein (LDL) for whole blood perfusion

A low-density lipoprotein and adsorbent technology, which is applied in the fields of polymer chemistry and biomedicine, can solve the problems of the limitation of ligand immobilization, affecting the adsorption effect of the adsorbent on the target substance, and the limited removal effect, and achieves excellent adsorption performance. Effect

Active Publication Date: 2015-04-08
JAFRON BIOMEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since phosphate is a small molecular substance, the removal effect on low-density lipoprotein and other target substances is limited. At the same time, the phosphate ligand is selective to the carrier, and some carriers with excellent performance cannot be used, and the immobilization capacity of the ligand is also limited. In turn, affect the adsorption effect of the adsorbent on the target substance

Method used

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  • Preparation method of adsorbent for adsorbing low-density lipoprotein (LDL) for whole blood perfusion
  • Preparation method of adsorbent for adsorbing low-density lipoprotein (LDL) for whole blood perfusion

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Preparation of the adsorbent for adsorbing low-density lipoprotein for whole blood perfusion:

[0044] Synthesis of Macroporous Polystyrene Adsorbent Resin:

[0045] Add 600mL of aqueous solution containing 0.5wt% polyvinyl alcohol and 3wt% NaCl to a 1000mL three-necked flask, add 25g styrene, 25g divinylbenzene (DVB) with a purity of 63wt%, 25g toluene, 35g 200 # The mixed organic phase of gasoline and 0.5g benzoyl peroxide (BPO) was heated to 75°C for 5 hours under mechanical stirring, and then heated to 80°C for overnight reaction. After the reaction was completed, it was cooled, extracted with acetone for 12 hours, and washed with water until There is no acetone smell, suction filtration, drying, sieving, and selecting the resin with a particle size of 0.6-1.2mm to obtain the required macroporous polystyrene adsorption resin. The specific surface area (BET) of the obtained macroporous polystyrene adsorption resin is 343m 2 / g, the pore volume is 0.79cm 3 / g, the ...

Embodiment 2

[0060] Preparation of the adsorbent for adsorbing low-density lipoprotein for whole blood perfusion:

[0061] Synthesis of Macroporous Polystyrene Adsorbent Resin:

[0062] Add 600mL of aqueous solution containing 0.5wt% polyvinyl alcohol and 3wt% NaCl to a 1000mL three-necked flask, add 5g styrene, 45g divinylbenzene (DVB) with a purity of 63wt%, 50g toluene, 30g n-heptane, 0.5 g Mixed organic phase of benzoyl peroxide (BPO), under mechanical stirring, heat up to 75°C for 5 hours, then heat up to 80°C for overnight reaction, after the reaction, cool down, extract with acetone for 12 hours, wash with water until there is no acetone smell , suction filtration, and drying to obtain a macroporous polystyrene adsorption resin; select the resin with a particle size of 0.6 to 1.2mm, and the specific surface area (BET) of the obtained macroporous polystyrene adsorption resin is 415m 2 / g, the pore volume is 1.02cm 3 / g, the average pore diameter is 9.83nm.

[0063] After synthesiz...

Embodiment 3

[0077] Preparation of the adsorbent for adsorbing low-density lipoprotein for whole blood perfusion:

[0078] Synthesis of Macroporous Polystyrene Adsorbent Resin:

[0079] Add 500mL aqueous solution containing 0.5wt% polyvinyl alcohol and 3wt% NaCl to a 1000mL three-necked flask, add 25g styrene, 10g divinylbenzene (DVB) with a purity of 63wt%, 21g toluene, 21g n-heptane, 0.35 g Mixed organic phase of benzoyl peroxide (BPO), under mechanical stirring, heat up to 75°C for 5 hours, then heat up to 80°C for overnight reaction, after the reaction, cool down, extract with acetone for 12 hours, wash with water until there is no acetone smell , suction filtration, and drying to obtain a macroporous polystyrene adsorption resin; select resins with a particle size of 0.6 to 1.2mm, and the specific surface area (BET) of the obtained macroporous polystyrene adsorption resin is 165m 2 / g, the pore volume is 0.67cm 3 / g, the average pore diameter is 16.24nm.

[0080] After synthesizing...

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Abstract

The invention relates to a preparation method of an adsorbent for adsorbing low-density lipoprotein (LDL) for whole blood perfusion. The preparation method comprises the following steps: performing chloromethylation modification and hydroxylation modification on polystyrene adsorption resin, then performing epichlorohydrin activation to ensure that the surface of the polystyrene macroporous adsorption resin carries a great quantity of active groups, and further immobilizing polyanion ligands onto the polystyrene macroporous adsorption resin. The whole blood perfusion adsorbent with excellent adsorption performance on LDL, TG (triglyceride) and TC (total cholesterol) is prepared by combining the electronegativity of polyanion, the hydrophobicity of a polystyrene resin skeleton structure and the pore adsorption action of the macroporous adsorption resin.

Description

technical field [0001] The invention relates to the fields of biomedicine and polymer chemistry, in particular to a method for preparing an adsorbent for absorbing low-density lipoprotein for whole blood perfusion. Background technique [0002] Cardiovascular disease is one of the three major diseases that threaten human health, and atherosclerosis is an important factor leading to the occurrence of cardiovascular disease. Studies have shown that high concentrations of low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL) in human blood are the main cause of atherosclerosis. In addition, high levels of total cholesterol (TC) and triglycerides (TG) in patients with hyperlipidemia are also prone to atherosclerosis. [0003] Reducing the concentration of LDL, TC and TG in the blood can effectively reduce the incidence of atherosclerosis, thereby effectively preventing and treating cardiovascular diseases. In recent years, more and more blood purification tech...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/30
CPCB01J20/265
Inventor 董凡邵柯
Owner JAFRON BIOMEDICAL
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