Preparation method and application of a single-channel dual-concentration gradient microfluidic chip

Abstract

The invention discloses a single-channel double-concentration-gradient microfluidic chip which comprises a side channel, a main channel and a PDMS (polydimethylsiloxane) film positioned between the side channel and main channel, wherein the side channel is a chemical oxygenation reaction channel, of which both ends are respectively provided with a reactant inlet and an outlet; a mixing region and a reaction region are sequentially arranged between the reactant inlet and outlet; the main channel is a cell culture channel; both ends of the main channel are respectively provided with an inlet and an outlet; and an oxygen gradient and a drug concentration gradient are formed on the main channel. The invention also discloses a preparation method and application method of the chip, establishes a double-gradient model on oxygen concentration and chemical drug concentration, and is applicable to tumor cell drug screening.

Description

technical field [0001] The invention relates to the technical field of microfluidics, the field of drug screening and the field of biological detection, in particular to a microfluidic chip capable of realizing double gradients of oxygen concentration and chemical concentration in a single channel. Background technique [0002] At present, most drug screening investigation methods ignore the hypoxic microenvironment of tumor cells in the body. Because the growth state of the cells is very different from the microenvironment in the body, the accuracy of the drug efficacy test is not high, and the receptor restricted by many factors. In various human cancers, solid tumors account for more than 90%. Some cells in solid tumors are in a hypoxic or anaerobic microenvironment. Tumor cells in a hypoxic microenvironment are resistant to chemical drugs. The treatment is resistant, not only because the cells under hypoxic conditions are resistant to most anti-tumor drugs, but also bec...

AI Technical Summary

Problems solved by technology

For example, L.Wang et al. constructed a microfluidic chip (Lab Chip, 2013, 13, 695–705.) with a dual concentration gradient of oxygen gradient and chemical concentration gradient for cell research. However, this microfluidic device oxygen Gradients are created by adding oxygen-consuming reagents directly to the cell culture medium, chemical reagents that change the composition of the cell culture medium and are more likely to affect cell behavior

Recently, Wen Chang et al. improved the generation of oxygen concentration and prepared a PDMS-PC microfluidic chip with dual concentration gradients of oxygen gradient and chemical concentration gradient in a non-toxic way to cells (Chia-Wen Chang, Yung-Ju Cheng, Melissa Tu, Ying-Hua Chen, Chien-Chung Peng, Wei-Hao Liaoa, Yi-Chung Tung, Lab Chip, 2014, 14, 3762–3772), but the chip has a three-layer complex structure and is cumbersome to operate

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