Preparation method of novel tetanus toxoid medium

Abstract

The invention discloses a preparation method of a novel tetanus toxoid medium. The medium is prepared by peptone, yeast powder, vitamins and inorganic salts through the corresponding preparation method. The problems of large hydrolysis degree difference, low quality consistence, difficult purification of toxins, and unstable toxin output in the prior art are solved, and the tetanus toxoid medium prepared in the invention has the advantages of hydrolysis difference reduction, quality consistence improvement, easy purification of the toxins, and stable increase of the output of the toxins.

Description

technical field [0001] The invention relates to a preparation method of a microbial culture medium, in particular to a preparation method of a novel tetanus toxoid culture medium for cultivating tetanus strains to obtain tetanus toxoid. Background technique [0002] Tetanus toxoid is an important preparation for the preparation of tetanus vaccine. Tetanus toxoid is inoculated with tetanus strains in the culture medium and extracted from the tetanus bacillus obtained by culture; The key to obtaining high-quality, efficient, stable, and consistently purified tetanus toxoid; the medium used for inoculating tetanus strains, cultivating tetanus bacillus, and obtaining tetanus toxoid, referred to herein as the medium. The prior art uses enzymes or acids to hydrolyze animal and plant proteins such as beef, casein, and soybeans to prepare medium; The production of toxin in the medium decreases, and the shallow hydrolysis makes the toxin produced in the existing medium contain exces...

AI Technical Summary

Problems solved by technology

The prior art uses enzymes or acids to hydrolyze animal and plant proteins such as beef, casein, and soybeans to prepare medium; The production of toxin in the medium decreases, and the shallow hydrolysis makes the toxin produced in the existing medium contain excessive impurity protein, which makes it difficult to purify the toxin; and the excessive impurity protein in the toxin will cause the final toxoid product to cause adverse effects on the human body reaction; therefore, there are problems and deficiencies in the prior art, such as large differences in degree of hydrolysis, poor quality consistency, difficult purification of toxins, and unstable toxin production