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Preparation method of polyurethane foam immobilized microbe for treatment of azo dye sewage

A technology of immobilized microorganisms and polyurethane foam, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of low decolorization effect of immobilized microorganisms, and achieve the effect of improved effect and high specific surface area

Inactive Publication Date: 2015-04-08
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for preparing polyurethane foam immobilized microorganisms for treating azo dye sewage, which solves the problem that the existing calcium alginate as an immobilized carrier has a low decolorization effect of immobilized microorganisms

Method used

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  • Preparation method of polyurethane foam immobilized microbe for treatment of azo dye sewage
  • Preparation method of polyurethane foam immobilized microbe for treatment of azo dye sewage
  • Preparation method of polyurethane foam immobilized microbe for treatment of azo dye sewage

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preparation example Construction

[0018] The present invention handles the preparation method of the polyurethane foam immobilized microorganism of azo dye sewage, and concrete steps are as follows:

[0019] Step 1, polyester polyol, triethanolamine, water, methyl silicone oil, dibutyltin dilaurate, and graphite felt powder after crushing are ultrasonically dispersed together for 5-10 seconds and then stirred and mixed evenly (polyester polyol, triethanolamine , water, methyl silicone oil, dibutyltin dilaurate, diphenylmethane diisocyanate MDI, the volume ratio between the crushed graphite felt powder is 100:2:2:2:1:100:1), and then Add diphenylmethane diisocyanate MDI, stir for 3-5 seconds until it turns white, and quickly transfer for 24 hours of natural foaming to obtain polyurethane foam. Cut the prepared polyurethane foam into small rectangular parallelepiped pieces with the same side length;

[0020] Step 2: Wash the polyurethane foam obtained in step 1 with distilled water for several times and then dry...

Embodiment 1

[0031] Step 1, 40ml of polyester polyol, 0.8ml of triethanolamine, 0.8ml of water, 0.8ml of methyl silicone oil, 0.3ml of dibutyltin dilaurate, and 3 pieces of crushed graphite felt powder (3×3cm) are ultrasonically dispersed together Stir and mix evenly after 5 seconds, then add 50ml of diphenylmethane diisocyanate MDI, stir for 5 seconds until it turns white, transfer it quickly for natural foaming for 24 hours, and obtain polyurethane foam, cut the prepared polyurethane foam into small cuboids with the same side length piece;

[0032]Step 2: Wash the polyurethane foam obtained in step 1 several times with distilled water and dry it to prepare LB medium for lysing broth. Put the polyurethane foam into the LB medium and sterilize it at 115°C for 30 minutes. After cooling, inoculate immobilized microorganisms Stenotrophomonas maltophilia strain T-8 was cultured with shaking at 25°C and 120rpm for 3 days, washed with distilled water several times, and polyurethane foam with imm...

Embodiment 2

[0034] Step 1, 40ml of polyester polyol, 0.8ml of triethanolamine, 0.8ml of water, 0.8ml of methyl silicone oil, 0.3ml of dibutyltin dilaurate, and 3 pieces of crushed graphite felt powder (3×3cm) are ultrasonically dispersed together Stir and mix evenly after 8 seconds, then add 50ml of diphenylmethane diisocyanate MDI, stir for 4 seconds until it turns white, transfer it quickly for natural foaming for 24 hours, and obtain polyurethane foam. Cut the prepared polyurethane foam into small cuboids with the same side length piece;

[0035] Step 2: Wash the polyurethane foam obtained in step 1 with distilled water for several times and then dry it to prepare LB medium for lysing broth. Put the polyurethane foam into the LB medium and sterilize it at 120°C for 25 minutes. After cooling, inoculate immobilized microorganisms Stenotrophomonas maltophilia strain T-8 was cultured with shaking at 30°C and 130rpm for 4 days, washed with distilled water several times, and polyurethane foa...

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Abstract

The invention relates to a preparation method of a polyurethane foam immobilized microbe for treatment of azo dye sewage. The preparation method comprises the following steps: performing ultrasonic dispersion on polyester polyol, triethanolamine, water, methyl silicone oil, dibutyltin dilaurate and graphite felt powder after grinding together, then adding diphenylmethane diisocyanate (MDI), stirring until whitening, rapidly transferring, naturally foaming to obtain polyurethane foam, washing with distilled water for several times, then drying, preparing a lysogeny broth (LB) culture medium, putting the polyurethane foam into the LB culture medium, sterilizing together, cooling, then inoculating an immobilized microbe, namely a Stenotrophomonas maltophilia strain T-8, performing shaking culture and washing with the distilled water for several times for preparation. The polyurethane foam with the immobilized microbe prepared by the preparation method provided by the invention has a macroporous reticular structure, high specific surface area and hydroxyl and other groups, and can effectively immobilize the microbe and greatly improve the effect of decoloring azo dye.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a preparation method of polyurethane foam immobilized microorganisms for treating azo dye sewage. Background technique [0002] In recent years, due to increasingly stringent environmental protection standards and policies, azo dye wastewater has attracted increasing attention due to its large output and difficult treatment. Immobilized cell technology is widely used in the treatment of azo dye wastewater, especially for the bioaugmentation of toxic and difficult-to-transform substances, because it can maintain a large density of cells, maintain high-efficiency bacterial activity, and has a strong ability to withstand the impact of toxic substances. Processing has enormous potential. Calcium alginate is the most widely used immobilized carrier due to its low cost and high activity. High concentration electrolyte (K + , Na + ) unstable in solution;...

Claims

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Application Information

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IPC IPC(8): C12N11/08C02F3/34C08G18/76C08G18/66C08G18/42C08G18/61C08K3/04C08G101/00C12R1/01
Inventor 朱超马宏瑞花莉解井坤
Owner SHAANXI UNIV OF SCI & TECH
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