Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Matrix and system for preserving biological specimens for qualitative and quantitative analysis

A biological sample and matrix technology, applied in the direction of analysis materials, biological testing, application, etc., can solve the problems of micro-extraction difficulties and achieve the effect of reducing the cost of transporting infectious materials

Inactive Publication Date: 2015-04-08
VIVEBIO
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, microextraction of genetic material from DBS or DPS on filter paper is significantly more difficult if uptake of high molecular weight DNA or RNA is required
While the introduction of new materials and shipping methods continues to improve the way samples are handled, the quantity and quality of samples available for subsequent analysis remains a major concern for researchers and clinicians alike

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Matrix and system for preserving biological specimens for qualitative and quantitative analysis
  • Matrix and system for preserving biological specimens for qualitative and quantitative analysis
  • Matrix and system for preserving biological specimens for qualitative and quantitative analysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0130] One (1.0)ml Sample Preparation and Device Recovery Kit

[0131] Kit components:

[0132] This example provides a kit for the preparation, transport and recovery of thirty-six (36) dried biological samples from bodily fluids or tissues. Materials and reagents for preparation and recovery of thirty-six (36) one (1.0) ml samples for dry environment shipment consisted of the following components:

[0133] components

quantity

Device kit container (tube)

36 each

Recovery buffer

3X 13ml

Disposable 3ml syringe

36 each

15ml conical centrifuge tube

36 each

[0134] Storage and Handling:

[0135] Upon receipt, store all kit components dry at room temperature (15-25°C). Only use the unit container tube when the indicating desiccant color is blue. Device kit containers should not be used if the indicator desiccant is white or pink in color. Such as 1000 μl pipette, 1000 μl sterile DNase-free with aerosol barrier, ...

Embodiment 2

[0137] Sample preparation using device kits

[0138] Sample preparation steps are performed in a biosafety cabinet using aseptic technique and general precautions related to the handling of potentially infectious materials. Before starting the sample preparation process, one should be familiar withFigure 1A Procedure for using the device kit described in &1B.

[0139] Before loading the sample liquid suspension of the biological sample containing the analyte of interest, uncap the device container, turn it over, and place the absorbent matrix up on the clean work surface ( Figure 1A &1B). Add approximately up to 1 ml of sample liquid slowly to the top of the matrix plug and allow to absorb completely into the matrix. Allow the device kit matrix loaded with sample liquid to air dry. Generally speaking, it takes about 4.5 to 5 hours to air dry in a biological safety cabinet. Once the sample is completely dry, the cap holding the dried sample-containing matrix is ​​carefully ...

Embodiment 3

[0141] Sample recovery using device kits

[0142] The sample recovery procedure was likewise performed in a biosafety cabinet using aseptic technique and usual precautions related to the handling of potentially infectious materials. Essentially, a sterile 3 or 5 ml disposable LUER-LOK syringe (supplied with the kit) is inserted into the 15 ml receiving tube (also supplied with the kit). Remove the plunger from the syringe barrel. The absorbent matrix containing the dried sample is transferred to the syringe by pressing the matrix against the sterile interior of the syringe barrel with just enough pressure to detach it from the attached cap and allow it to fall freely to the bottom of the syringe. In the syringe barrel ( Figure 4 &5). Place the syringe cylinder with the removed matrix plug into the 15 ml conical receiver tube, which is further placed into the rack. Slowly and directly apply approximately 1 ml of reconstitution buffer (supplied with the kit) to the top of t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
diameteraaaaaaaaaa
sizeaaaaaaaaaa
Login to View More

Abstract

The present invention provides a device, system, and methods of use comprising an absorbent hydrophobic polyolefin matrix, and methods of use thereof, for storage, preserving, and recovering liquid suspension of biological specimens containing analytes of interest in a dry state. The dried biological specimens containing analytes of interest absorbed on the polyolefin matrix are reconstituted such as with molecular-grade water and released by compressing the polyolefin matrix. The reconstituted biological analytes are qualified for subsequent analysis, such as for qualitative and quantitative analysis of viral nucleic acids, such a viral load testing, genotyping, and sequencing. Also provided are kits with instructions, and methods of use thereof, for storage, preserving, and recovering biological specimens containing analytes of interest using the compression device of the invention.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to US Provisional Application No. 61 / 680,193, filed August 6, 2012, which is hereby incorporated by reference in its entirety. field of invention [0003] The present invention generally relates to biological sample preservation matrices and systems, devices and methods of use thereof. More specifically, the present invention relates to substrates and systems for the collection, storage and recovery of nucleic acids, such as viral DNA and RNA samples, for subsequent quantitative and qualitative laboratory analysis, such as viral load, genetic analysis, type and antiviral drug resistance testing. Background of the invention [0004] Biological samples are often collected, transferred, and stored in order to analyze the levels and concentrations of the various analytes contained therein. By convention, liquid suspensions of biological samples are stored under refrigeration in airtight, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/04G01N1/00C12Q1/68G01N33/48
CPCC12Q1/6806G01N2001/4027A61B10/0096
Inventor 阿贝尔·德拉罗萨米米·C·G·希利克里斯蒂·S·莉丝丹尼尔·R·麦克伦阿妮塔·马修斯·麦克伦
Owner VIVEBIO
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com