Phytolacca americana extract and application of phytolacca americana extract in preventing and treating dalbergia odorifera anthracnose

A technology of Dalbergia balsamicus and its extracts, applied in the direction of application, chemicals for biological control, biocides, etc., to achieve low cost, good control effect, and simple preparation process

Active Publication Date: 2015-04-22
HAINAN KEDA FORESTRY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the biological so

Method used

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  • Phytolacca americana extract and application of phytolacca americana extract in preventing and treating dalbergia odorifera anthracnose
  • Phytolacca americana extract and application of phytolacca americana extract in preventing and treating dalbergia odorifera anthracnose
  • Phytolacca americana extract and application of phytolacca americana extract in preventing and treating dalbergia odorifera anthracnose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Wash Pokeweed and place it in a dry and ventilated place to dry in the shade to constant weight, put it into a pulverizer and crush it, and pass through a 40-mesh sieve. The obtained powder was placed in an oven at 40°C for 24 hours and then sealed and stored. Use ethyl acetate as the extraction solvent, the ratio of solid to liquid is 1g:10ml, soak 3 times at room temperature, each soaking time is 3d, collect the filtrate 3 times, carry out rotary evaporation, concentrate into extract to constant weight, and obtain ethyl acetate extraction thing.

[0022] A certain amount of ethyl acetate extract of pokeweed was weighed, dissolved in an appropriate amount of absolute ethanol, and then distilled water was added to make the ethanol concentration 10% and the extract concentration 100 mg / ml.

[0023] Add an appropriate amount of 100mg / ml ethyl acetate extract of Pokeweed to the sterilized 55°C PDA medium to make the extract concentration 3.125mg / ml, 6.25mg / ml, 12.5mg / ml, ...

Embodiment 2

[0033] The preparation method of each extract is the same as that in Example 1. A certain amount of Pokeweed ethyl acetate extract is weighed, an appropriate amount of absolute ethanol is added to dissolve, and then distilled water is added, so that the ethanol concentration is 10%, and the extract concentration is 100mg / ml .

[0034] Add an appropriate amount of 100mg / ml plant extract to the sterilized 55°C PDA medium to make the extract concentration 2.5mg / ml, 5mg / ml, 7.5mg / ml, 10mg / ml, 12.5mg / ml Toxic culture medium, with an equal volume of 10% ethanol solution as the control, repeat 3 culture dishes for each treatment. Pipette 0.2ml of the bacterial suspension into the poisonous petri dish and the control petri dish, spread evenly, and culture at 28°C. After 2 days, the growth of pathogenic bacteria was observed, and the minimum concentration of no bacterial growth was the MIC of the extract.

[0035] Continue to cultivate the above pathogenic bacteria, and continue to o...

Embodiment 3

[0040] The preparation method of each extract is the same as that in Example 1. A certain amount of Pokeweed ethyl acetate extract is weighed, an appropriate amount of absolute ethanol is added to dissolve, and then distilled water is added, so that the ethanol concentration is 10%, and the extract concentration is 100mg / ml .

[0041] Take 6 Erlenmeyer flasks, add the same amount of PD medium, add appropriate amount of plant extract after sterilization, so that the final concentration is EC 50 , Mixed and inoculated with 0.2ml anthracnose pathogenic spore suspension. 160r / min, shaking culture at 28°C. After culturing for 48h, 60h, 72h, 84h, 96h, and 120h, take out an Erlenmeyer flask, filter with suction, and place the obtained bacteria in an oven at 60°C to dry until constant weight. The operation was repeated three times, and a blank control group without drug solution was set up. Weigh the dry weight of the bacteria and draw the growth curve.

[0042] Depend on figure ...

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Abstract

The invention discloses a phytolacca americana extract and the application of the phytolacca americana extract in preventing and treating the dalbergia odorifera anthracnose. The extract is extracted from phytolacca americana by means of ethyl acetate. The phytolacca americana extract and the application of the phytolacca americana extract in preventing and treating the dalbergia odorifera anthracnose have the advantages that the required phytolacca americana is an invasive plant and is cheap and easy to obtain, and other plant resources are prevented from being wasted; only one kind of plant is needed, a quite good preventing and treating effect can be achieved in a monothetic mode instead of a compound mode, the preparing processes are simple, and cost is low; the effective constituents are compounds synthesized by the plant, automatic degradation is achieved, resistibility caused by bacterium breeding is avoided, and harm to the environment is avoided.

Description

technical field [0001] The invention relates to a plant extract capable of inhibiting the pathogenic bacteria of Dalbergia japonica anthracnose and an application method thereof. The invention belongs to the field of plant extracts and their antibacterial activity. Background technique [0002] Dalbergia Dalbergia, also known as huanghuali, is an endangered species, a specialty of Hainan, and an evergreen tree of the Fabaceae Papilionaceae subfamily. Anthracnose is one of the most important diseases of Dalbergia japonica, which causes the leaves to gradually dry up or perforate until they fall off, seriously affecting the photosynthesis of the plant and the formation of the core material, and often outbreaks in large areas. At present, the prevention and treatment of Dalbergia japonica anthracnose in China is mainly chemical control technology. For example, Liu Hongwan and others reviewed the control methods using 80% anthrax thiram, 75% chlorothalonil or Bordeaux mixture. ...

Claims

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Application Information

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IPC IPC(8): A01N65/08A01P3/00
Inventor 周国英周洁尘刘君昂董文统李河李杨苏圣淞
Owner HAINAN KEDA FORESTRY CO LTD
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