Active component of selaginella doederleinii hieron as well as preparation method and use thereof

Abstract

The invention discloses an active component of selaginella doederleinii hieron as well as a preparation method and a use thereof. The method comprises the following steps: extracting and concentrating degreased residues of selaginella doederleinii hieron by using an ethanol solution to obtain selaginella doederleinii hieron liquid extract; and repeatedly dissolving the selaginella doederleinii hieron liquid extract in water, filtering the solution by using macroreticular resin, gradient eluting the solution by using different polar solutions, collecting to obtain different polar eluents of selaginella doederleinii hieron, concentrating and carrying out tumor cells in vitro screening to obtain an antitumor active component. The active constituent enriches more than 70% of flavonoid components of the selaginella doederleinii hieron, and the active component of the selaginella doederleinii hieron can be prepared into a variety of dosage forms by pharmaceutical methods. The active component of the selaginella doederleinii hieron prepared from the preparation method is provided to have an obvious inhibitory effect on such tumor cell lines as lung cancer, leukemia, colon cancer, nasopharyngeal carcinoma and the like in vitro. The active component prepared from the preparation method can be applied to preparation of antitumor drugs or pharmaceutical compositions.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract, in particular to a method for preparing an active component of cypress cypress and its application. Background technique [0002] Selaginella is a genus of Selaginella ( Selaginella ) plant dark green Selaginella ( Selaginella doederleinii The dried whole herb of Hieron). It is mainly produced in Fujian, Taiwan, Guangdong and other places. It can be harvested throughout the year, washed, used fresh or dried as medicine. It has the effects of clearing away heat and detoxification, expelling wind and dampness, stopping bleeding, and anticancer. It is an anticancer drug commonly used in anticancer prescriptions of traditional Chinese medicine. Modern pharmacological studies have also found that Shishangbai or its prescriptions can enhance the body's metabolism and reticuloendothelial system function in cancer patients, while no reports of toxic side effects have been seen. [0003] S...

AI Technical Summary

Problems solved by technology

At present, there is no report on the development and research of new drugs on the anti-tumor active compounds and components of cypress cypress, and there are disadvantages such as unclear material basis and uncontrollable quality, and there is a lack of methods and patent reports for the preparation of active components of high-content total flavonoids