Method for separating and extracting 20(S)-protopanaxatriol from cortex juglandis mandshuricae and use of 20(S)-protopanaxatriol

A technology for the separation of protopanaxadiol and its separation method, which is applied in the field of extraction and separation of 20-protopanaxadiol, which can solve the problems of unfavorable industrial production, undetailed description of the separation process, and environmental pollution, so as to save the amount of extraction solvent and process Ease of use and save extraction time

Inactive Publication Date: 2015-04-29
HEILONGJIANG UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Wild walnut resources are abundant, and the annual output of walnut green skin is more than 100,000 tons, but most of them are discarded as waste, which not only pollutes the environment, but also fails to make full use of this precious resource (Yang Jinzhi, Chen Jinping. Comprehensive development of walnut resources Utilization, Food and Drugs, 2007, 9(4): 71-73)
[0003] Zhou Yuanyuan isolated 20(S)-protopanaxadiol from Qinglongyi for the first time (published, Yang Bingyou, Meng Ying, Liu Zhaoxi, Jiang Yanqiu, Zhou Yuanyuan. Analysis of triterpenoids in Qinglongyi, Chinese Journal of Experimental Formulas, 2015 , 21(2):49-52), but the specific separation process is not described in detail and the process has not been perfected, the yield is low, which is not conducive to industrial production

Method used

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  • Method for separating and extracting 20(S)-protopanaxatriol from cortex juglandis mandshuricae and use of 20(S)-protopanaxatriol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] in a blue dragon garment 20(S)- The extraction and separation method of protopanaxadiol is characterized in that: comprising the following steps:

[0017] (1) Extraction: Take Qinglongyi medicinal material collected in August of that year as the raw material, dry it at low temperature and weigh 5kg; add dichloromethane 50KHz ultrasonic extraction 3 times according to the amount of solvent that is 5 times the weight of the raw material (V / W). After 45 minutes, dichloromethane was reclaimed under reduced pressure to obtain 138 g of dichloromethane extract;

[0018] (2) Impurity removal: Add 138g of dichloromethane extract to water to adjust to a suspension with a density of 1.05 g / mL. The suspension is passed through the activated carbon suction filter funnel for 10 times for vacuum suction filtration (activated carbon particles The particle size is between 40 mesh and 60 mesh, and the volume of activated carbon should not exceed 1 / 2 volume of the height of the Buchn...

Embodiment 2

[0022] in a blue dragon garment 20(S)- The extraction and separation method of protopanaxadiol is characterized in that: comprising the following steps:

[0023] (1) Extraction: take 5kg of the dry medicinal material prepared in Example 1; add dichloromethane 50KHz ultrasonic extraction 8 times, 45min each time, and recover dichloromethane under reduced pressure , to obtain 162g of dichloromethane extract;

[0024] (2) Impurity removal: 162g of dichloromethane extract was added to water, adjusted to a suspension with a density of 1.05 g / mL, and the suspension was repeatedly passed through the activated carbon decompression device in Example 1 for 8 times. The purpose of the process is mainly to remove fat-soluble impurities mainly based on chlorophyll; after the suction filtration is completed, the suction filtrate is concentrated and evaporated to dryness, then add 30ml of absolute ethanol, 100g of Girard reagent P 100g and 12% (V / V) acetic acid to knead and dissolve. W...

Embodiment 3

[0028] in a blue dragon garment 20(S)- The extraction and separation method of protopanaxadiol is characterized in that: comprising the following steps:

[0029] (1) Extraction: Take 5kg of the dry medicinal material prepared in Example 1; add dichloromethane 50KHz ultrasonic extraction 4 times, each time for 45min, and recover dichloromethane under reduced pressure , to obtain 158g of dichloromethane extract;

[0030] (2) Impurity removal: 158g of dichloromethane extract was added to water to adjust to a suspension with a density of 1.05 g / mL, and the suspension was repeatedly passed through the activated carbon decompression device in Example 1 for 5 times. The purpose of the process is mainly to remove fat-soluble impurities mainly based on chlorophyll; after the suction filtration is completed, the suction filtrate is concentrated and evaporated to dryness, then add 30ml of absolute ethanol, 100g of Girard reagent P 100g and 12% (V / V) acetic acid to knead and dissolve...

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Abstract

The invention relates to method for separating and extracting 20(S)-protopanaxatriol from cortex juglandis mandshuricae and use of 20(S)-protopanaxatriol and discloses a method for separating and extracting 20(S)-protopanaxatriol from cortex juglandis mandshuricae and use of 20(S)-protopanaxatriol. The method comprises the following steps of carrying out ultrasound extraction on cortex juglandis mandshuricae medicinal material which is harvested in current year with dichloromethane, adsorbing with activated carbon and a Girard reagent to remove impurity and separating by the silica gel column chromatography and the like to obtain the target product 20(S)-protopanaxatriol. The whole process is simple and feasible. In addition, the invention also relates to application of 20(S)-protopanaxatriol in preparing drugs for treating mucosal injury caused by chronic gastric ulcer and the comprehensive utilization value of cortex juglandis mandshuricae can be increased.

Description

technical field [0001] The invention relates to a method for extracting and separating 20(S)-protopanaxadiol, in particular to a method for extracting and separating 20(S)-protopanaxadiol in Qinglongyi and its application. Background technique [0002] 20(S)-Protopanaxadiol is a tetracyclic triterpenoid compound. Modern pharmacological studies have shown that a variety of such diol group ginsenosides have antitumor effects. Metabolism studies have found that ginsenogenin - protopanaxadiol and its metabolites in vivo have good anti-tumor activity. And compared with other ginsenosides, protopanaxadiol has stronger antitumor activity (Liu Na, Park Huri, Li Ning, etc. Research progress on chemistry and anticancer activity of protopanaxadiol and its derivatives, Chinese Medicinal Chemistry Journal, 2008, 8(5): 384-388). In the past, this ingredient was mainly isolated from plants of the genus Panax genus or obtained through complex chemical synthesis, and the cost of raw materi...

Claims

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Application Information

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IPC IPC(8): C07J9/00A61P1/04
CPCA61K31/575C07J9/00
Inventor 周媛媛付蕾张晓娟
Owner HEILONGJIANG UNIV OF CHINESE MEDICINE
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