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Plant extract and method for cross-linking collagen by using plant extract as biological cross-linker

A technology of plant extract and biological cross-linking agent is applied in the field of plant extract and its use as a biological cross-linking agent to cross-link collagen to achieve the effects of improving stability and broad application prospects

Active Publication Date: 2015-04-29
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These compounds can effectively cross-link collagen, but due to their chemical toxicity, they should not be used in food processing

Method used

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  • Plant extract and method for cross-linking collagen by using plant extract as biological cross-linker

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Experimental program
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Effect test

Embodiment 1

[0044] A plant extract, Amomum chinensis extract, contains polyphenol active ingredients, and Amomum chinensis extract is in the form of dry powder. The specific preparation method steps of Amomum chinensis extract are as follows:

[0045] In the first step, the amomum is washed with deionized water, dried at 60°C, and crushed to 80 mesh with a pulverizer;

[0046] In the second step, the pulverized amomum powder is leached in 50% ethanol solution, the ratio of feeding material to liquid is 1:15, the leaching temperature is 60°C, the leaching time is 2 hours, and continuous stirring is required during the leaching process. ;

[0047] In the third step, the leaching solution and the residue are filtered and separated with a 0.5 μm membrane;

[0048] In the fourth step, the leaching solution is concentrated under reduced pressure, the temperature is 60°C, and the vacuum degree is 0.07MPa;

[0049] In the fifth step, the concentrated plant extract solution is quickly frozen at...

Embodiment 2

[0057] A plant extract, amomum extract, contains polyphenol active ingredients, and the amomum extract is in the form of dry powder. The specific preparation method steps of the amomum extract are as follows:

[0058] In the first step, the amomum is washed with deionized water, dried at 70°C, and crushed to 120 mesh with a pulverizer;

[0059] In the second step, the pulverized amomum powder is leached in 75% ethanol solution, the ratio of feeding material to liquid is 1:20, the leaching temperature is 50°C, and the leaching time is 4 hours. During the leaching process, constant stirring is required. ;

[0060] In the third step, the extract and residue are centrifuged at a speed of 5000r / min, and the supernatant is taken;

[0061] The fourth step is to concentrate the extract under reduced pressure at a temperature of 50°C and a vacuum of 0.08MPa;

[0062] In the fifth step, the obtained concentrated plant extract solution is frozen at -18°C, and after freezing, a dehydra...

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Abstract

The invention discloses a plant extract and a method for cross-linking collagen by using the plant extract as a biological cross-linker. The plant extract is an amomum villosum extract, wherein the amomum villosum extract contains polyphenol effective components. The plant extract is prepared through the following steps: firstly, cleaning, drying and crushing amomum villosum; secondly, extracting crushed amomum villosum powder in an extracting solution; thirdly, separating the extracting solution; fourthly, concentrating the extracting solution; fifthly, obtaining a concentrated plant extract solution or dehydrated plant extract powder. The plant extract has the benefits that by using the amomum villosum plant extract as the biological cross-linker, molecular chains of the collagen are cross-linked, the structure of the collagen can be stabilized, and the degradation resistance of the collagen is improved, so that the processing characteristics of a collagen product are improved, the physical properties and the texture characteristics are good, and the application prospect is wide.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a plant extract and a method for crosslinking collagen as a biological crosslinking agent. Background technique [0002] Collagen exists in the connective tissue of animals and the human body, and is an important structural matrix for tissues, cartilage, skin and other organs. Animal collagen has rich nutritional value and is often extracted and used as a functional food raw material. However, due to the collagen itself The triple helix structure of collagen is easy to degrade, and it is difficult to maintain the stable triple helix structure of collagen, thus affecting the texture characteristics and thermal stability of foods with collagen as ingredients. Therefore, in order to improve the stability and anti-degradation ability of collagen, it is now necessary to cross-link collagen to generate collagen with a stable structure, thereby improving its anti-degradation ability and ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08L89/00A23L1/30A23L33/105
Inventor 薛勇赵园园薛长湖王玉明李兆杰徐杰唐庆娟王静凤
Owner OCEAN UNIV OF CHINA
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