Anti-calcification biological patch preparation method
A technology of biological patch and water injection, applied in the field of biomedical materials, can solve the problems of inability to enhance the antibacterial and bacteriostatic function of cross-linked materials, dark color of cross-linked materials, and difficulty in large-scale production, and achieves excellent anti-calcification ability, good Anti-degradation performance, excellent effect of hemostatic performance
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Embodiment 1
[0021] 1. Weigh 100 grams of acellular porcine dermal matrix into the conical flask, add 100 grams of injection water into the conical flask, then place the conical flask in a temperature-controlled cradle filled with water, and turn on the heating switch , adjust the temperature to 37°C and shake for 10 minutes;
[0022] 2. Weigh 8 grams of epoxy chitosan quaternary ammonium salt and add it to the Erlenmeyer flask, shake for 20 minutes until the epoxy chitosan quaternary ammonium salt dissolves completely and penetrates into the acellular porcine dermal matrix;
[0023] 3. Weigh 10 grams of sodium hydroxide and dissolve it in 50 grams of water to obtain a sodium hydroxide solution;
[0024] 4. Slowly add sodium hydroxide solution dropwise, adjust the pH of the reaction solution to 10.0±0.5, keep warm and shake for 72 hours;
[0025] 5 Slowly add lactic acid dropwise, adjust the pH of the reaction solution to neutral, and pour off the reaction solution;
[0026] 6. Add 300 g...
Embodiment 2
[0030] 1. Weigh 10Kg fetal cowhide acellular dermal matrix into the drum, add 30Kg of water for injection, turn on the heating switch, adjust the temperature of the drum to 47°C, and rotate for 30 minutes;
[0031] 2. Add 2Kg epoxy chitosan quaternary ammonium salt to the drum and rotate for 60 minutes;
[0032] 3. Weigh 1Kg of sodium carbonate, slowly add it into the drum, and continuously check the pH value of the reaction, until the pH reaches 9.0-9.5, do not add sodium carbonate; then continue to rotate, and react for 24 hours;
[0033] 4. Weigh 1Kg of ammonium chloride, slowly add it into the drum, and continuously check the pH value of the reaction until the pH reaches 7.0±0.5, then pour off the reaction solution;
[0034] 5. Add 10Kg of water for injection, rotate for 120 minutes, and pour off the reaction solution;
[0035] 6. Then add 10Kg of water for injection, rotate for 120 minutes, and pour off the reaction solution;
[0036] 7. Add 1Kg RPMI-1640 medium, rotate...
Embodiment 3
[0040] 1. Weigh 50Kg of fetal calfskin acellular dermal matrix and put it into the drum, add 25Kg of water for injection, turn on the heating switch, adjust the temperature of the drum to 20°C, and rotate for 20 minutes;
[0041] 2. Add 1Kg epoxy chitosan quaternary ammonium salt to the drum and rotate for 30 minutes;
[0042] 3. Add boric acid-borax buffer solution, adjust the pH to 8.0±0.5, and rotate for 48 hours;
[0043] 4. Slowly add acetic acid dropwise, while continuously checking the pH value of the reaction until the pH reaches 7.0, then pour off the reaction solution;
[0044] 5. Add 50Kg of water for injection, rotate for 60 minutes, and pour off the reaction solution;
[0045] 6. Add 150Kg of water for injection, rotate for 120 minutes, and pour off the reaction solution;
[0046] 7. Add 10Kg RPMI-1640 medium, rotate for 2 hours, pour out the medium;
[0047]8. Add 250Kg of water for injection, wash for 2 hours, pour off the cleaning solution; then add 250Kg of...
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