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A kind of yeast agent and its use and method for producing ethanol

A technology of yeast and yeast, which is applied in the field of yeast inoculum and ethanol production, can solve the problems of being unable to become the dominant strain of lignocellulosic ethanol, inhibiting cell growth and fermentation performance, and inhibiting sensitive components, etc., to achieve strong practicability and sugar The effect of high utilization rate and stable genetic performance

Active Publication Date: 2018-01-05
CHINA PETROLEUM & CHEM CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Saccharomyces cerevisiae can efficiently use glucose to produce ethanol, it has poor tolerance to inhibitors in fermentation broth and high concentration of ethanol (Almeida J R M et al., 2007), especially sensitive to inhibitory components in cellulose hydrolyzate , preventing Saccharomyces cerevisiae from becoming the dominant strain for lignocellulosic ethanol production
Secondly, in the production of cellulosic ethanol, the degradation products produced during the pretreatment of cellulose raw materials and the residual degradation products will inhibit the subsequent fermentation of strains to varying degrees, among which acetic acid, furfural, phenols and SO 4 2- and other inhibitory factors have a strong toxic effect on yeast, which will significantly inhibit the growth of cells and their fermentation performance

Method used

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  • A kind of yeast agent and its use and method for producing ethanol
  • A kind of yeast agent and its use and method for producing ethanol

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Embodiment approach

[0022] According to a preferred embodiment of the present invention, the preparation method of the yeast agent is as follows: inoculating Saccharomyces cerevisiae RIPP-06, Saccharomyces cerevisiae RIPP-01 and Saccharomyces cerevisiae RIPP-07 into a yeast medium for culturing. The yeast preparation prepared according to the preferred embodiment of the present invention has better fermentation performance.

[0023] Wherein, there is no special limitation to the total inoculation amount of above-mentioned three kinds of Saccharomyces cerevisiae, can be conventional inoculum amount, preferably, with respect to the total sugar of every gram in described yeast culture medium, the total inoculum amount of Saccharomyces cerevisiae is 10 3 -10 8 For each yeast cell, more preferably, the ratio of the inoculation amount of Saccharomyces cerevisiae RIPP-06, Saccharomyces cerevisiae RIPP-01 and Saccharomyces cerevisiae RIPP-07 is 1:0.5-2:0.5-2. There is no particular limitation on the yea...

preparation Embodiment 1

[0040] Prepare the saccharomyces agent according to the proportion shown in Table 1.

[0041] Table 1

[0042]

preparation Embodiment 2

[0044] Saccharomyces cerevisiae RIPP-06, Saccharomyces cerevisiae RIPP-01 and Saccharomyces cerevisiae RIPP-07 were inoculated into yeast medium (ie seed medium without acetic acid) for cultivation (cultivation temperature was 30°C and cultivation time was 50h), Saccharomyces cerevisiae The total inoculum size is 10 5 yeast cells (relative to the total sugar per gram in the yeast culture medium), the inoculum ratio of S. cerevisiae RIPP-06, S. cerevisiae RIPP-01 and Saccharomyces cerevisiae RIPP-07 is 1:1:1 to obtain yeast Agent 4 # .

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Abstract

The invention discloses a yeast inoculant which contains saccharomyces cerevisiae RIPP-06, saccharomyces cerevisiae RIPP-01 and saccharomyces cerevisiae RIPP-07, wherein the collection number of the saccharomyces cerevisiae RIPP-06 is CGMCC No.8203, the collection number of the saccharomyces cerevisiae RIPP-01 is CGMCC No.8201, and the collection number of the saccharomyces cerevisiae RIPP-07 is CGMCC No.8204. The invention also discloses application of the yeast inoculant in production of ethanol. Besides, the invention also discloses a method for producing ethanol. The method comprises the following step of: carrying out ethanol fermentation on an ethanol fermentation stock solution by using the yeast inoculant. The yeast inoculant disclosed by the invention has the advantages of high stress resistance, multiple stress tolerance, stable genetic property, high sugar utilization rate, high ethanol output and high practicability and can be applied to the existing fuel ethanol production process and new lignocellulose ethanol process.

Description

technical field [0001] The invention relates to a yeast agent, its use in ethanol production and a method for producing ethanol by using the yeast agent. Background technique [0002] Due to the rapid increase in the use of traditional fossil energy, energy shortage has become a worldwide problem. The development of energy diversification and the acceleration of renewable energy development have become the research and development priorities of countries all over the world. Among them, the production and application of fuel ethanol are important in economic development and strategic security. out of great significance. The diversification strategy of energy supply represented by alternative energy such as fuel ethanol has become an important direction of my country's energy policy. Cellulose is a very abundant and cheap biomass raw material that can be degraded into fermentable sugars for the production of fuel ethanol. Vigorously developing biomass fuel ethanol as a renew...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18C12P7/06C12P7/10C12R1/865
CPCC12N1/18C12P7/06C12P7/10Y02E50/10
Inventor 高岚刘颖郭勇刘金胜李宝石蔺建民
Owner CHINA PETROLEUM & CHEM CORP
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