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A method for identifying Cordyceps sinensis powder mixed with fermented Cordyceps sinensis powder

A technology of Cordyceps sinensis powder and Cordyceps sinensis, which is applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of market adulteration, difficulty in identifying Cordyceps sinensis fermented Cordyceps sinensis powder, flooding of counterfeit products, etc., to achieve fast and accurate identification, convenient batch identification, The effect of strong economic applicability

Active Publication Date: 2017-07-07
YICHANG SHANCHENGSHUIDU CORDYCEPS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But its efficacy and price are quite different from wild Cordyceps sinensis
Therefore, many unscrupulous traders added fermented Cordyceps sinensis fungus powder and other similar products to Cordyceps sinensis in order to make huge profits.
[0004] Microscopic identification and infrared identification of pure powder of Cordyceps sinensis reported in the current literature can effectively identify and distinguish whether non-genuine Cordyceps such as Yaxiangbang Cordyceps and Liangshan Cordyceps are mixed in or mixed with soil, glue, starch, etc., but cannot Effectively identify whether Cordyceps sinensis is mixed with its analog - fermented Cordyceps sinensis powder
Because the chemical composition of wild Cordyceps sinensis and fermented Cordyceps sinensis powder is very similar, traditional methods such as thin layer chromatography and high performance liquid chromatography are also difficult to identify the low proportion of fermented Cordyceps sinensis powder mixed in Cordyceps sinensis

Method used

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  • A method for identifying Cordyceps sinensis powder mixed with fermented Cordyceps sinensis powder
  • A method for identifying Cordyceps sinensis powder mixed with fermented Cordyceps sinensis powder
  • A method for identifying Cordyceps sinensis powder mixed with fermented Cordyceps sinensis powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take a commercially available Cordyceps sinensis capsule sample A, take out the powder, grind and pulverize it, pass through a 100-mesh sieve, then weigh 0.050g of the sample and place it in a 100mL round-bottomed conical flask, add 10mL of 30% ethanol aqueous solution, 40KH Z Frequency ultrasonic extraction for 25min, centrifugation at 5000rpm for 10min to obtain the supernatant. Take out the supernatant and dilute it 4 times, filter it with a 0.45 μm microporous membrane, and scan the ultraviolet spectrum with 12.5 μg / mL adenosine standard as a reference. The scanning conditions are: scanning range 350-220nm, spectral resolution 2nm. The resulting spectrum is shown in figure 1 . It can be seen from the ultraviolet absorption spectrum of the sample that there is no negative absorption value in the 275-250nm band, indicating that the fermented Cordyceps sinensis powder is mixed in the sold Cordyceps sinensis capsules.

Embodiment 2

[0026] Take a commercially available Cordyceps sinensis buccal tablet sample B, grind it and pass through a 100-mesh sieve, then weigh 0.050g of the sample and place it in a 100mL round-bottomed conical flask, add 10mL of 30% ethanol aqueous solution, 40KH Z Frequency ultrasonic extraction for 25min, centrifugation at 5000rpm for 10min to obtain the supernatant. Take out the supernatant and dilute it 4 times, filter it with a 0.45 μm microporous membrane, and scan the ultraviolet spectrum with 12.5 μg / mL adenosine standard as a reference. The scanning conditions are: scanning range 350-220nm, spectral resolution 2nm. The resulting spectrum is shown in figure 2 . It can be seen from the ultraviolet absorption spectrum of the sample that there is a negative absorption value in the 275-250nm band, indicating that more than 10% fermented Cordyceps sinensis powder is not mixed in the sold Cordyceps sinensis buccal tablets.

Embodiment 3

[0028] Take the commercially available Cordyceps capsule sample A, the wild Cordyceps standard product and the fermented Cordyceps powder standard product purchased from China Food and Drug Control Institute and the Cordyceps powder containing 10%, 20%, 40%, 50% fermented Cordyceps powder standard product 7 samples were crushed and passed through a 100-mesh sieve respectively, then weighed 0.050g each, placed in a 100mL round-bottomed Erlenmeyer flask, added 10mL of 30% ethanol aqueous solution, extracted by ultrasonic at 40KHZ frequency for 25min, centrifuged at 5000rpm for 10min to take the supernatant liquid. Take out the supernatant and dilute it 4 times, filter it with a 0.45 μm microporous membrane, take the 12.5 μg / mL adenosine standard solution as a reference, and scan the ultraviolet spectrum of the above 7 samples respectively. The conditions are as follows: Range 350-220nm, spectral resolution 2nm. The ultraviolet scanning spectra obtained by superimposing the wild...

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Abstract

The invention relates to an ultraviolet spectrum analysis identification method for whether fermented cordyceps sinensis powder is mixed into cordyceps sinensis powder and for preliminary judgment of the mixing ratio. Using the adenosine solution with a concentration of 12.5 μg / mL as the reference solution, scan the ultraviolet spectrum at 350‑220 nm. Observing whether there is a negative absorption value in the 275-250nm range of the sample to be tested can determine whether the fermented Cordyceps sinensis powder is mixed. The scanning patterns of Cordyceps pure powder sample, fermented Cordyceps sinensis powder standard substance and Cordyceps sinensis samples containing different proportions of fermented Cordyceps sinensis powder standard substance were superimposed to establish a standard model pattern. Comparing the spectrum of the sample to be tested with the established model spectrum can preliminarily judge the proportion of mixed fermented Cordyceps sinensis powder. The identification method of the invention has a small demand for samples, simple operation, fast analysis, easy operation, no need for large expensive instruments, accurate and reliable identification results, and batch identification.

Description

technical field [0001] The invention relates to an identification method of Chinese medicinal materials, in particular to an analysis method for identifying whether or not fermented Cordyceps sinensis powder is mixed in the powder of Cordyceps sinensis and for preliminary judgment of the mixing ratio. [0002] technical background [0003] Cordyceps sinensis (Berk.) Sacc. (also known as Cordyceps sinensis, Chinese caterpillar fungus) is a complex of ergot fungus parasitizing on the larvae of bat moths and larvae. Cordyceps sinensis grows in plateaus and high mountains at an altitude of 3,500 to 5,000 meters, and is distributed in Sichuan, Qinghai, Tibet, and Yunnan in my country. The tradition of Cordyceps sinensis was first recorded in "Materia Medica Congxin". It is sweet in taste and warm in nature, enters the lung and kidney meridian, and has the effects of nourishing the lung and kidney, relieving cough, benefiting deficiency and insufficiency, and strengthening essence....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33
Inventor 李文佳艾中钱正明周建桥李文庆李光荣
Owner YICHANG SHANCHENGSHUIDU CORDYCEPS
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