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Application of ADAMTS1 as aortic dissection clinical screening molecular marker

A molecular marker, arterial dissection technology, applied in the field of medicine and biology

Active Publication Date: 2015-04-29
郑金刚 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Whether ADAMTS-1 is involved in the degradation of vascular wall matrix protein versican, thereby promoting the occurrence and development of aortic dissection, has not been reported yet

Method used

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  • Application of ADAMTS1 as aortic dissection clinical screening molecular marker
  • Application of ADAMTS1 as aortic dissection clinical screening molecular marker
  • Application of ADAMTS1 as aortic dissection clinical screening molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Enzyme-linked immunosorbent assay (ELISA) was used to detect plasma ADAMTS1 levels in patients with acute aortic dissection (AAD) (16 cases), patients with acute myocardial infarction (AMI) (26 cases) and normal people (19 cases).

[0017] Collection criteria for patients with aortic dissection: Symptoms: sudden severe chest and back tear-like pain, accompanied by uncontrollable hypertension; auxiliary examination: Aortic CT or MRI shows that the aorta has a false cavity.

[0018] The specific conditions of each group of cases are shown in the table below:

[0019]

[0020] The ELISA test showed that the plasma ADAMTS1 level is 0.87±0.12 ng / ml in normal people, the plasma ADAMTS1 level in patients with myocardial infarction is 0.98±0.14 ng / ml, and the plasma ADAMTS1 level in patients with aortic dissection is 1.44±0.25 ng / ml,

[0021] The plasma ADAMTS1 level of patients with aortic dissection was significantly higher than the first two groups. Statistical analysis by Oneway-A...

Embodiment 2

[0024] Example 2 Research on Mouse Model of Aortic Dissection

[0025] A mouse aortic dissection model was successfully established with angiotensin II (Ang II) implanted pump administration method. Male C57 mice aged 8-9 months were implanted with a slow-release pump subcutaneously in the back. The administration group was pumped with angiotensin II 2500 ng / kg / min, and the control group was pumped with normal saline. The samples were taken 14 days after the pump was buried. Artery, extract total RNA for real-time quantitative PCR, extract total protein for Western Blot, fix and embed the slice for HE staining, elastic plate staining, immunohistochemistry and immunofluorescence.

[0026] The results are as follows:

[0027] The blood pressure (SBP) of mice in the AngII-administered group was significantly higher than that in the control group. The incidence of dissection in the AngII-administered group was 42%, and the incidence of dissection in the control group was 0.

[0028] ...

Embodiment 3

[0031] Take the plasma samples of 150 patients with acute chest pain, and test the plasma samples according to the detection method in Example 1. The content of ADAMTS1 is> 1.2ng / ml is used as the criterion, and if it is higher than 1.2ng / ml, it is determined that the patient has an aortic dissection. The test results showed that the ADAMTS1 content of 69 samples was> 1.2ng / ml, CT examination of the aorta showed that the 69 samples were all AD patients.

[0032] Other tests proved that among the other 81 samples, 8 were aortic aneurysms, 6 were aortic intramural hematomas, 41 were acute coronary syndromes, 4 were pulmonary embolism, and 22 were chest pains caused by other causes. CT proved that there were no patients with aortic dissection in the 81 samples, indicating that the diagnostic accuracy of ADAMTS1 was 100%.

[0033] In addition, according to the non-patent literature Wu Wenjing et al., a comparative study of plasma ADAMTS-9 levels in patients with aortic dissection and a...

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Abstract

The invention belongs to the field of medicine bioengineering and in particular relates to an application of ADAMTS1 as an aortic dissection clinical screening molecular marker. Many researches show that protein expression quantity of ADAMTS1 is highly correlated to aortic dissection; and a risk that a person suffers from aortic dissection is obviously improved along with increase of the protein expression quantity of ADAMTS1. By virtue of the application, ADAMTS1 content in blood plasma of a patient suffering from the aortic dissection is found to be at least 50% higher than that of a normal person.

Description

technical field [0001] The invention belongs to the field of medical biology, and in particular relates to the application of ADAMTS1 as a molecular marker for clinical screening of aortic dissection. Background technique [0002] Aortic dissection (Aortic dissection, AD) is one of the serious life-threatening cardiovascular emergencies. It refers to the tear of the aortic intima. The walls of such torn blood vessels are very weak and prone to dilation or rupture, which is often fatal. About 36%-50% of clinically untreated patients with aortic dissection died within 48 hours after onset, about 65%-75% died within two weeks, the one-year mortality rate was as high as 90%, and the 10-year mortality rate was almost 100%. (1) At present, interventional therapy and artificial vascular replacement are the main methods for the treatment of ruptured aortic dissection. Despite the continuous development of surgical methods, the overall morbidity and mortality have not improved; (2...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 郑金刚高艳香吴文静于长安
Owner 郑金刚