Method for preparing fermented product filtrate by fermenting rice with lactobacillus casei, and application of fermented product filtrate

A technology of Lactobacillus casei and fermented products, applied in the field of Lactobacillus casei fermenting rice to prepare fermented product filtrate

Inactive Publication Date: 2015-05-06
MEIJIALE SHANGHAI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] There are few reports on the application of lactic acid bacteria fermented rice to skin care. The present invention specifically descri

Method used

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  • Method for preparing fermented product filtrate by fermenting rice with lactobacillus casei, and application of fermented product filtrate
  • Method for preparing fermented product filtrate by fermenting rice with lactobacillus casei, and application of fermented product filtrate
  • Method for preparing fermented product filtrate by fermenting rice with lactobacillus casei, and application of fermented product filtrate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] The preparation of embodiment 1 Lactobacillus casei rice fermentation product filtrate

[0066] The medium contains 20% rice and 80% drinking water, and the two are mixed and kept at 70° C. for 20 minutes. After cooling, inoculate Lactobacillus casei at 27-40°C for static culture for 24 hours. At this time, the pH value of the fermentation system is less than 4, and the filtrate is obtained by filtering with a 40-mesh sieve, adding arginine to the filtrate to adjust the pH value to 5.0-6.0, and sterilizing at 80°C-105°C for 20 minutes to obtain the fermentation product filtrate.

Embodiment 2

[0067] Embodiment 2 Penetration-promoting effect test

[0068] 1 material

[0069] 1.1 Epidermal stem cells were isolated and cultured in vitro to obtain a three-dimensional skin reconstruction model.

[0070] 1.2 Instruments High performance liquid chromatography LC-VP (Shimadzu, Japan); constant temperature gas bath.

[0071] 1.3 Drug reagent Ascorbyl glucoside; the fermentation product obtained in Example 1; all reagents used are analytically pure.

[0072] 2 methods

[0073] 2.1 Use high performance liquid chromatography to analyze and determine the content of ascorbic acid.

[0074] 2.2 Release solutions A to C use physiological saline as a solvent.

[0075] Group A is a control group without penetration enhancer, which is 2.0% by weight of ascorbyl glucoside physiological saline solution;

[0076] Group B adopts the fermented product filtrate prepared in Example 1, which is a composition physiological saline solution of 5.0% by weight, and wherein the concentration ...

Embodiment 3

[0084] Embodiment 3 acute toxicity test

[0085] The rice fermentation product filtrate of Lactobacillus casei in Example 1 was orally administered to each of five male and female mice in an amount of 10 g / kg, and when observed, it was confirmed that there were no deaths and abnormalities.

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Abstract

The invention discloses a method for preparing fermented product filtrate by fermenting rice with lactobacillus casei, and the application of the fermented product filtrate. The method comprises the steps of firstly, carrying out heat sterilization on a culture medium; then, inoculating the lactobacillus casei into the culture medium, fermenting, and filtering to obtain the fermented product filtrate; after that, adjusting the pH value of the fermented product filtrate, and sterilizing to obtain the fermented product filtrate. After being used in the compositions of cosmetics, the fermented product filtrate has a good function of promoting the permeation of other components, the skin wrinkles can be reduced, and the skin melanin is reduced; the fermented product filtrate is high in biological safety, thus being widely applied to various cosmetics.

Description

technical field [0001] The invention relates to the field of cosmetics, in particular to a method for preparing a fermented product filtrate by fermenting rice with Lactobacillus casei and its application. Background technique [0002] Rice, also known as "rice". It is mainly divided into indica rice, japonica rice and sticky rice. Rice is flat in nature and sweet in taste. It has the functions of invigorating the middle and nourishing the stomach, benefiting the essence and strengthening the will, improving the ears and eyesight, harmonizing the five internal organs, clearing the four veins, relieving restlessness, quenching thirst, and antidiarrheal. [0003] Traditional Chinese medicine believes that rice is sweet and flat, and has the effects of invigorating the spleen and nourishing the stomach, strengthening the will, improving eyesight, and is known as "the first of the five grains". Li Shizhen, a medical scientist in the Ming Dynasty, said in "Compendium of Materi...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/97A61Q19/00A61Q19/02
Inventor 张文源
Owner MEIJIALE SHANGHAI BIOTECH CO LTD
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