Application of cipadessa cinerascens A and analogues thereof in preparation for anti-depression medicines or foods
A technology of antidepressant and neem berry, applied in the field of preparation of antidepressant drugs or food, to achieve the effect of small side effects and definite curative effect
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Embodiment 1
[0026] The preparation of embodiment 1 neem A
[0027] Neem A is a monomer isolated and extracted from Neem, with a purity of more than 98% through identification.
[0028] Method for Extraction, Separation and Purification of Neem A
[0029] Take about 20 Kg of crushed neem medicinal material (fruit or seeds), and extract by cold immersion in 95% ethanol. Each soaking time is one week, and the extraction is 3 times. The extract was filtered and concentrated under reduced pressure to a paste to obtain an extract. Suspend the extract in water, extract with petroleum ether, dichloromethane and ethyl acetate respectively, and concentrate the extract under reduced pressure to obtain partial extracts of petroleum ether, partial extracts of dichloromethane and partial extracts of ethyl acetate. The chemical components of the above extracts were separated by silica gel column chromatography, dextran gel, preparative thin-layer method and preparative high-performance liquid chromato...
Embodiment 2
[0033] Embodiment 2 mouse forced swimming experiment model
[0034] Male Kunming mice were used as experimental animals, and the mice had free access to food and water, with a body weight of about 30 grams during the experiment.
[0035] Put a certain amount of neem A in a mortar, add a quantitative amount of 0.5% sodium carboxymethyl cellulose solution and grind it into a suspension, and make a mixture of neem A and 0.5% sodium carboxymethyl cellulose suspension; the positive control drug was venlafaxine. The way of administration is intragastric administration.
[0036] Mice were randomly divided into 5 groups, divided into negative control group (vehicle group), low-dose Neem pilosa A group (5mg / kg), medium-dose Neem A high-dose group (50 mg / kg), venlafaxine positive control group (10 mg / kg), 9-10 rats in each group.
[0037] Such as figure 1As shown, the mice began to be administered after 5 days of adaptive feeding, and the day of the first administration was counted ...
Embodiment 3
[0042] Example 3 Mouse tail suspension experiment
[0043] Male Kunming mice were used as experimental animals, and the mice had free access to food and water, with a body weight of about 30 grams during the experiment.
[0044] With 0.5% sodium carboxymethyl cellulose as the solvent, fresh neem A-0.5% sodium carboxymethyl cellulose suspension was prepared before the experiment; the positive control drug was venlafaxine. The way of administration is intragastric administration.
[0045] Mice were randomly divided into 5 groups, divided into negative control group (vehicle group), low-dose Neem pilosa A group (5mg / kg), medium-dose Neem A high-dose group (50 mg / kg), venlafaxine positive control group (10 mg / kg), 8-10 rats in each group. Such as figure 1 As shown, the mice began to be administered after 5 days of adaptive feeding, and the day of the first administration was counted as the first day of administration, and the administration was continued for 7 days, and the beh...
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