Brown planthopper survival and growth development-associated NlPIK3R1 gene, and coded protein and application thereof

Abstract

The invention discloses a brown planthopper survival and growth development-associated NlPIK3R1 gene, and a coded protein and an application thereof. The gene NlPIK3R1 for coding a brown planthopper phosphatidyl inositol 3 kinase p85 alpha subunit comprises a nucleotide sequence as shown in SEQ ID NO:1 and a homologous mutant sequence thereof. The gene has an important function in a survival and growth development process of the brown planthopper, and is restrained in function to cause brown planthopper survival rate drop, slow growth and delayed adult eclosion. The protein coded by the brown planthopper NlPIK3R1 gene comprises an amino acid sequence as shown in SEQ ID NO:2 and a homologous mutant sequence thereof. The gene is restrained in function to cause brown planthopper survival rate drop, slow growth and delayed adult eclosion. RNA interference of the brown planthopper NlPIK3R1 gene is applied to control brown planthopper. RNA interference is successfully performed on the gene, the survival rate of pests is decreased according to the results, the survival and the growth development are obviously restrained, the weight is reduced, and the adult eclosion is delayed.

Description

technical field [0001] The invention relates to a gene related to survival, growth and development of brown planthopper, encoded protein and application thereof. Background technique [0002] Brown planthopper (Nilaparvata lugens ) is a long-distance migratory rice pest, which mainly damages rice plants by sucking phloem sap and spreading rice viruses. In recent years, the brown planthopper has developed into an important pest in the rice region of Asia, with an annual damage area of ​​20 million hectares 2 More than billions of kilograms of paddy output losses are caused (Lin Yongjun et al., 2011). At present, the main emergency control measures against brown planthoppers are spraying chemical pesticides. The extensive abuse of chemical pesticides not only seriously pollutes the environment, but also induces the resistance of brown planthoppers to the long-term application of chemical pesticides (Wang Peng et al., 2013). Among the pesticides used to control brown plant...

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Problems solved by technology

The massive abuse of chemical pesticides not only seriously pollutes the environment, but also induces resistance in brown planthoppers (Wang Peng et al., 2013)

Among the pesticides used to control brown planthoppers, the effective pesticides that have played a good role in the past, such as fipronil, imidacloprid, buprofezin, etc., have been eliminated or restricted in use. When carrying out prevention and control, I feel helpless in the choice of pesticides, and chemical control is facing an embarrassing situation that cannot be prevented

The reason is that most of these chemical pesticides aim at large-scale and high-intensity killing of pests. However, due to practical reasons such as the extremely complex genetic diversity of the brown planthopper population, some of them can survive. As a result of high selection pressure, eventually lead to the formation of more adaptive populations

On the other hand, while chemical pesticides kill pests, they will also endanger non-target organisms including natural enemies, which will inevitably have a negative impact on the farmland ecosystem, and the ecological control function has not waited for its due performance

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