Method for detecting scutellarin content

A technology of scutellarin and scutellarin, which is applied in the field of analytical chemistry, can solve the problems of inaccurate detection, long detection cycle, and high detection cost, and achieve the effects of good reproducibility, low detection cost, and simple method

Inactive Publication Date: 2015-05-06
KPC PHARM INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The quality standard of scutellarin has been included in the "Chinese Pharmacopoeia" 2010 edition. The content of scutellarin (or scutellarin) is detected by the method of HPLC external standard reference substance. This method is exclusive and accurate, but there is a detection cos

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  • Method for detecting scutellarin content
  • Method for detecting scutellarin content
  • Method for detecting scutellarin content

Examples

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Example Embodiment

[0025] Example 1: The method of the present invention for detecting the content of scutellarin

[0026] Weigh the test product containing scutellarin, completely dissolve it with 0.1 mol / L sodium hydroxide aqueous solution, and then titrate with 0.1 mol / L hydrochloric acid. The Swiss Meton potentiometric titrator indicates the end of the reaction, and the first acid solution is recorded ( Hydrochloric acid) consumption volume;

[0027] Take the same volume of the 0.1mol / L sodium hydroxide aqueous solution, titrate with 0.1mol / L hydrochloric acid to the end of the reaction, and record the consumption volume of the second acid solution (hydrochloric acid);

[0028] Calculate according to the following formula to get the percentage of scutellarin:

[0029]

Example Embodiment

[0030] Example 2: Method for detecting scutellarin content of the present invention

[0031] Weigh the test product containing scutellarin, completely dissolve it with 0.01mol / L potassium hydroxide aqueous solution, and then titrate with 0.01mol / L sulfuric acid. The Swiss Meton potentiometric titrator indicates the end of the reaction and records the first acid solution ( Sulfuric acid) consumption volume;

[0032] Take the same volume of 0.01mol / L potassium hydroxide aqueous solution, titrate with 0.01mol / L sulfuric acid to the end of the reaction, and record the consumption volume of the second acid solution (sulfuric acid);

[0033] Calculate according to the following formula to get the percentage of scutellarin:

[0034]

Example Embodiment

[0035] Example 3: Method for detecting scutellarin content of the present invention

[0036] Weigh the test product containing scutellarin, completely dissolve it with 0.05mol / L sodium hydroxide aqueous solution, and then titrate with 0.05mol / L phosphoric acid. The Swiss Meton potentiometric titrator indicates the end of the reaction and records the first acid solution ( Phosphoric acid) consumption volume;

[0037] Take the same volume of 0.05mol / L sodium hydroxide aqueous solution, titrate with 0.05mol / L phosphoric acid to the end of the reaction, and record the consumption volume of the second acid solution (phosphoric acid);

[0038] Calculate according to the following formula to get the percentage of scutellarin:

[0039]

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Abstract

The invention relates to the field of analytical chemistry and discloses a method for detecting scutellarin content. The method comprises the following steps of weighing a scutellarin-containing product to be detected, completely dissolving the product in alkali lye, carrying out titration until a reaction terminal point by an acid solution, recording a consumed volume of a first acid solution, taking alkali lye with a volume the same as that of the used alkali lye, carrying out titration until a reaction terminal point by the acid solution, recording a consumed volume of a second acid solution, and carrying out calculation by the two consumed volumes according to a formula. The method can detect scutellarin content by an alkali residual titration method, is free of an expensive scutellarin contrast, is simple, fast and accurate, has good reappearance, greatly reduces a detection cost and is especially suitable for semi-finished product quantitative analysis and low-added value breviscapinun preparation analysis.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for detecting the content of scutellarin. Background technique [0002] Erigeron breviscapus (vant.) Hand.-Mazz. is the whole herb of Erigeron breviscapus (vant.) Hand.-Mazz.], also known as Erigeron breviscapus (vant.) Hand.-Mazz. This medicinal material was first recorded in "Southern Yunnan Materia Medica", and has been included in the 2005 edition of "Chinese Pharmacopoeia". Yunnan folks are often used to treat bruises, rheumatism pain, toothache, stomach pain, colds, etc. In the 1970s, it was clinically verified that it has good curative effect on hypertension, cerebral hemorrhage, cerebral thrombosis, multiple neuritis of cerebral embolism, chronic arachnoiditis and its sequelae, and also has certain curative effect on rheumatism and coronary heart disease. Medical research shows that the main active ingredient of breviscapine for treating cardiovascular and ce...

Claims

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Application Information

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IPC IPC(8): G01N31/16
Inventor 张伟王珺
Owner KPC PHARM INC
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