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Method for detecting scutellarin content

A technology of scutellarin and scutellarin, which is applied in the field of analytical chemistry, can solve the problems of inaccurate detection, long detection cycle, and high detection cost, and achieve the effects of good reproducibility, low detection cost, and simple method

Inactive Publication Date: 2015-05-06
KPC PHARM INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The quality standard of scutellarin has been included in the "Chinese Pharmacopoeia" 2010 edition. The content of scutellarin (or scutellarin) is detected by the method of HPLC external standard reference substance. This method is exclusive and accurate, but there is a detection cost High, lack of long detection cycle
Some literatures also use breviscapine and chlorine trichloride for color development, and use rutin as a reference substance, and use ultraviolet spectrophotometry to detect total flavonoids, but this method has poor specificity and inaccurate detection

Method used

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  • Method for detecting scutellarin content
  • Method for detecting scutellarin content
  • Method for detecting scutellarin content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: The present invention detects the method for scutellarin content

[0026] Weigh the test product containing scutellarin, dissolve it completely with 0.1mol / L sodium hydroxide aqueous solution, then titrate with 0.1mol / L hydrochloric acid, the Swiss Meton potentiometric titrator indicates the reaction end point, and record the first acid solution ( hydrochloric acid) consumption volume;

[0027] Take the 0.1mol / L aqueous sodium hydroxide solution of the same volume as above, titrate with 0.1mol / L hydrochloric acid to the end of the reaction, and record the consumed volume of the second acid solution (hydrochloric acid);

[0028] Calculate the percentage content of scutellarin according to the following formula:

[0029]

Embodiment 2

[0030] Embodiment 2: The present invention detects the method for scutellarin content

[0031] Weigh the test product containing scutellarin, dissolve it completely with 0.01mol / L potassium hydroxide aqueous solution, then titrate with 0.01mol / L sulfuric acid, the Swiss Meton potentiometric titrator indicates the reaction end point, and record the first acid solution ( sulfuric acid) consumption volume;

[0032] Take the above-mentioned same volume of 0.01mol / L potassium hydroxide aqueous solution, titrate with 0.01mol / L sulfuric acid to the end of the reaction, and record the consumption volume of the second acid solution (sulfuric acid);

[0033] Calculate the percentage content of scutellarin according to the following formula:

[0034]

Embodiment 3

[0035] Embodiment 3: The method for detecting the content of scutellarin in the present invention

[0036] Weigh the test product containing scutellarin, dissolve it completely with 0.05mol / L sodium hydroxide aqueous solution, then titrate with 0.05mol / L phosphoric acid, the Swiss Meton potentiometric titrator indicates the reaction end point, and record the first acid solution ( Phosphoric acid) consumed volume;

[0037] Take the 0.05mol / L aqueous sodium hydroxide solution of the same volume as above, titrate with 0.05mol / L phosphoric acid to the end of the reaction, and record the consumption volume of the second acid solution (phosphoric acid);

[0038] Calculate the percentage content of scutellarin according to the following formula:

[0039]

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Abstract

The invention relates to the field of analytical chemistry and discloses a method for detecting scutellarin content. The method comprises the following steps of weighing a scutellarin-containing product to be detected, completely dissolving the product in alkali lye, carrying out titration until a reaction terminal point by an acid solution, recording a consumed volume of a first acid solution, taking alkali lye with a volume the same as that of the used alkali lye, carrying out titration until a reaction terminal point by the acid solution, recording a consumed volume of a second acid solution, and carrying out calculation by the two consumed volumes according to a formula. The method can detect scutellarin content by an alkali residual titration method, is free of an expensive scutellarin contrast, is simple, fast and accurate, has good reappearance, greatly reduces a detection cost and is especially suitable for semi-finished product quantitative analysis and low-added value breviscapinun preparation analysis.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for detecting the content of scutellarin. Background technique [0002] Erigeron breviscapus (vant.) Hand.-Mazz. is the whole herb of Erigeron breviscapus (vant.) Hand.-Mazz.], also known as Erigeron breviscapus (vant.) Hand.-Mazz. This medicinal material was first recorded in "Southern Yunnan Materia Medica", and has been included in the 2005 edition of "Chinese Pharmacopoeia". Yunnan folks are often used to treat bruises, rheumatism pain, toothache, stomach pain, colds, etc. In the 1970s, it was clinically verified that it has good curative effect on hypertension, cerebral hemorrhage, cerebral thrombosis, multiple neuritis of cerebral embolism, chronic arachnoiditis and its sequelae, and also has certain curative effect on rheumatism and coronary heart disease. Medical research shows that the main active ingredient of breviscapine for treating cardiovascular and ce...

Claims

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Application Information

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IPC IPC(8): G01N31/16
Inventor 张伟王珺
Owner KPC PHARM INC
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