Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Thyroid microsomal antibody detection reagent kit as well as preparation method and application thereof

A technology of thyroid microsomes and kits, which is applied in the direction of biological testing, measuring devices, material inspection products, etc., can solve the problems of rare detection kits, and achieve the effects of long detection time, long storage time, and small system error

Active Publication Date: 2015-05-13
SHENZHEN NEW INDS BIOMEDICAL ENG
View PDF7 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently there are few detection kits with excellent performance for the detection of TMA by chemiluminescence immunoassay

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Thyroid microsomal antibody detection reagent kit as well as preparation method and application thereof
  • Thyroid microsomal antibody detection reagent kit as well as preparation method and application thereof
  • Thyroid microsomal antibody detection reagent kit as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] (1) Labeling of protein A

[0051] Preparation of dialysate (F solution): Add Na to a 5000ml beaker 2 CO 3 14.31g, NaHCO 3 26.46g, add purified water to dilute to 4500ml. The prepared F solution was placed on a magnetic stirrer for later use.

[0052] Choose a dialysis bag with a suitable cutoff (usually 14,000), measure the appropriate size, tie one end tightly after wetting, and test for leaks with purified water 3 times (no leakage is required).

[0053] Take 100 μg of protein A and adjust to 1 ml with 0.1 mol / L pH9.5 carbonic acid buffer (F solution). Put it into the dialysate, stir and dialyze at room temperature for 2 hours, add 300 μg of ABEI activated ester to the dialyzed solution, and react at 37°C for 2 hours.

[0054] The ligation product of protein A and ABEI was purified by G-25 gel column.

[0055] D. 2 Solution preparation: add 200ml of 0.5M phosphate buffer (P001 solution), 20g BSA, 8g NaN 3 , 2g MgCl 2 ·6H 2 O, 600ml glycerin, add purified wa...

Embodiment 2

[0073] (1) Protein A biotinylation, the specific steps are as follows:

[0074] Preparation of dialysate (F solution): Add Na to a 5000ml beaker 2 CO 3 14.31g, NaHCO 3 26.46g, add purified water to dilute to 4500ml. The prepared F solution was placed on a magnetic stirrer for later use.

[0075] Take 100 μg of biotin and 1 mg of protein A and adjust to 1 ml with 0.1 mol / L pH9.5 carbonic acid buffer (F solution). Put it into the dialysate, stir and dialyze at room temperature for 2 hours, and react at 37°C for 2 hours.

[0076] Purified by G-25 gel column.

[0077] D2 solution was prepared according to the method of Example 1, and the purified connection product was prepared with D 2 The solution was diluted to a concentration of 0.025 µg / ml.

[0078] (2) SA mark

[0079] Take 100μg SA and adjust it to 1ml with 0.1mol / L pH9.5 carbonic acid buffer (F solution). Put it into the dialysate, stir and dialyze at room temperature for 2 hours, add 300 μg of ABEI activated este...

Embodiment 3

[0096] (1) Labeling of protein A

[0097] Preparation of dialysate (F solution): Add Na to a 5000ml beaker 2 CO 3 14.31g, NaHCO 3 26.46g, add purified water to dilute to 4500ml. The prepared F solution was placed on a magnetic stirrer for later use.

[0098] Choose a dialysis bag with a suitable cutoff (usually 14,000), measure the appropriate size, tie one end tightly after wetting, and test for leaks with purified water 3 times (no leakage is required).

[0099] Take 100 μg of protein A and adjust to 1 ml with 0.1 mol / L pH9.5 carbonic acid buffer (F solution). Put it into the dialysate, stir and dialyze at room temperature for 2 hours, add 300 μg of ABEI activated ester to the dialyzed solution, and react at 37°C for 2 hours.

[0100] The ligation product of protein A and ABEI was purified by G-25 gel column.

[0101] D. 2 Solution preparation: add 200ml of 0.5M phosphate buffer (P001 solution), 20g BSA, 8g NaN 3 , 2g MgCl 2 ·6H 2 O, 600ml glycerin, add purified wa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to a chemical luminescent immunodetection thyroid microsomal antibody detection reagent kit and a preparation method of the reagent kit. The reagent kit comprises a protein A antigen labeled with a tracer marker, or an anti-human IgG and anti-human IgM labeled with the tracer marker, a thyroid microsomal antigen coated with magnetic spheres, or a junction complex of a protein carrier and the thyroid microsomal antigen coated with the magnetic spheres. The invention also provides a method for detecting the concentration of the thyroid microsomal antibody. When the reagent kit provided by the invention is used for detecting the concentration of the thyroid microsomal antibody, the enzymatic reaction is not involved, and the bridging of enzyme is not needed, so that the reagent is long in preservation time and stable; the detection also can be carried out by virtue of a full-automatic chemical luminescent method, so that the operation time is shortened, the manmade operation error is reduced, and the sensitivity and the accuracy in detection can be improved by utilizing the specificity of the chemical tracer marker.

Description

technical field [0001] The invention relates to a detection kit, in particular to a chemiluminescence immunoassay kit for detecting thyroid microsome antibody. The invention also relates to a preparation method of the kit and a method of using the kit to detect the concentration of thyroid microsome antibody. Background technique [0002] Thyroid microsomal antibody (TMA) is an antibody against thyroid microsomes (TM), which is one of the autoantibodies caused by autoimmune thyroid disease. TAM and antithyroglobulin antibody (TGA) have been recognized as important signs in the process of thyroid autoimmunity, and are the most representative antibodies. TAM and TGA are indispensable indicators in the diagnosis of autoimmune thyroid disease, and are one of the specific means for histological diagnosis of autoimmune thyroid disease. [0003] For various thyroid diseases, the positive detection rate of anti-TM: 50% to 100% for Hashimoto’s thyroiditis; 88.9% for hypothyroidism;...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68
CPCG01N33/532G01N33/564G01N33/68
Inventor 饶微徐红陈帆李武杨雅丽李婷华袁锦云
Owner SHENZHEN NEW INDS BIOMEDICAL ENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com