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Method for removing chlorophyll in microalgaes

A chlorophyll and removal technology is applied in the field of chlorophyll removal to achieve the effects of simplifying the process, improving the decolorization efficiency and reducing the cost

Active Publication Date: 2015-05-20
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of the present invention can remove high-content chlorophyll in microalgae with high efficiency, simplicity, safety and low cost, effectively solves the problem of chlorophyll pollution of microalgae products, and the decolorization waste liquid can be used for the preparation of sodium copper chlorophyllin, degreening microalgae Algae powder or wet algae mud can be used for the extraction and preparation of microalgae oil, protein, polysaccharides and carotenoids, and can also be used for the development of health care products, cosmetics, bio-energy, feed, bait, etc.

Method used

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  • Method for removing chlorophyll in microalgaes
  • Method for removing chlorophyll in microalgaes
  • Method for removing chlorophyll in microalgaes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A kind of green algae was selected to study the chlorophyll removal process. Through measurement, it is found that the chlorophyll content of the algae powder is 0.48g / 100g-1.20g / 100g, the algae powder before decolorization is yellow-green or green, and the algae oil is dark brown or black. Optimal decolorization conditions were obtained through process parameter optimization: the decolorization solution was obtained by mixing 100% ethanol with 1% to 2% sodium hydroxide solution according to the volume ratio of 1:4, and then the green algae powder According to the material concentration of 1% to 2%, it is mixed with the decolorizing solution and reacted at 70° C. for 1.0 to 2.0 hours to remove the chlorophyll from the algae powder.

[0031] By the method of this embodiment, the removal rate of chlorophyll is more than 90%, the algae powder after decolorization becomes orange-red or gray-yellow, the extracted microalgae oil is transparent orange-red or slightly yellow, a...

Embodiment 2

[0034] A diatom was chosen to study the chlorophyll removal process. It is found by measuring that the chlorophyll content of the algae powder is 0.32g / 100g, and the algae powder before decolorization is dark brown. Mix ethanol with a concentration of 100% and a sodium hydroxide solution with a concentration of 0.5% according to a volume ratio of 1:10 or 1:5 to obtain a decolorizing solution, and then diatom algae powder is mixed with the decolorizing solution according to a material concentration of 0.5%. React at 40°C for 3.0 hours to remove chlorophyll from the algae powder.

[0035] Through the method of this embodiment, the removal rate of chlorophyll can reach more than 90%.

[0036] The decolorized waste liquid in this example can be used for the preparation of sodium copper chlorophyllin, the diatom powder of chlorophyll can be used for the extraction and preparation of microalgae oil, protein, polysaccharide and carotenoid, and the diatom powder of chlorophyll can be...

Embodiment 3

[0038] A species of Eurythmia euphratus was chosen to study the chlorophyll removal process. The algae powder before decolorization is green or yellow-green, and the algae oil is dark brown. Mix ethanol with a concentration of 100% and potassium hydroxide solution with a concentration of 2% according to the volume ratio of 1:4 to obtain a decolorizing solution, and then mix the algae powder with the decolorizing solution according to the material concentration of 1% to 5%. , react at 70°C for 1 to 2 hours to remove chlorophyll from the algae powder.

[0039] By the method of this embodiment, the removal rate of chlorophyll can exceed 95%, the algae flour after degreening becomes pale yellow or orange, the microalgae oil becomes yellow or transparent orange, and the neutral lipid ratio of microalgae oil increases , the ratio of glycolipids and phospholipids is reduced.

[0040] The decolorization waste liquid in this example can be used for the preparation of sodium copper ch...

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Abstract

The invention discloses a method for removing chlorophyll in microalgaes. The method comprises the following steps: mixing microalgae powder or wet algae mud with a material concentration (mass-volume ratio) of 0.5-5.0% with a destaining solution, and reacting for 0.5-3.0 hours at a temperature of 40-70 DEG C, so that chlorophyll can be removed, wherein a preparation method of the destaining solution is implemented by mixing low carbon alcohol with an alkaline solution with a concentration of 0.5-2.5% in a certain volume ratio. The method disclosed by the invention can remove high-content chlorophyll in microalgaes in an efficient, simple, safe and low-cost mode, thereby effectively solving the chlorophyll pollution problem of microalgae products; and the destaining solution can be used for preparing sodium copper chlorophyllin, microalgae powder or wet algae mud after chlorophyll removal can be applied to the extraction and preparation of microalgae oil, proteins, polysaccharides and carotenoids, and related products can be applied to the development of health products, cosmetics, biological energy sources, feeds, baits and the like.

Description

Technical field: [0001] The invention belongs to the technical field of chlorophyll removal, and in particular relates to a method for removing chlorophyll from microalgae. Background technique: [0002] Chlorophyll is an important and indispensable component for microalgae to carry out photosynthesis and maintain normal cell life activities. Usually, the content of chlorophyll in microalgae can account for 0.2-2.0% of the dry weight of cells. Chlorophyll is a magnesium porphyrin compound with a high color value (greater than 500). It can appear green in a small amount. The presence of chlorophyll seriously affects the quality of microalgae end products (such as oil, protein, polysaccharides and carotenoids). The patent provides an efficient, simple, low-cost and safe process for removing chlorophyll from microalgae, which effectively solves the problem of chlorophyll pollution of microalgae products. The shortcoming of prior art and purpose of the present invention are det...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D487/22C11B1/00C07K1/14C08B37/04C07C403/02A23L1/29A61K8/97A23K1/14A23L33/00
Inventor 李涛向文洲王广华吴华莲戴世鲲何慧
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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