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A kind of method of biological control and anti-white thorn stem rot and growth promotion

A technology for stem rot and Nitraria, applied in the field of biological control fertilizer preparation, can solve the problems of poor resistance, low stress resistance and low stability of non-spore bacteria, and achieve simple treatment, good economic benefits, and easy operation Effect

Active Publication Date: 2017-12-22
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, non-spore bacteria have low stress resistance and poor resistance to dry high temperature, so the stability is not high when made into liquid or adsorbent

Method used

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  • A kind of method of biological control and anti-white thorn stem rot and growth promotion
  • A kind of method of biological control and anti-white thorn stem rot and growth promotion
  • A kind of method of biological control and anti-white thorn stem rot and growth promotion

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) High-density cultivation of Bacillus subtilis (CICC 10339) producing beta glucanase, the starting density of Bacillus subtilis (CICC 10339) is 1.18×10 6 Pieces / mL, inoculate into liquid culture medium (glucose 14g / L, beef extract 25g / L, potassium dihydrogen phosphate 2.45g / L, peanut meal powder 10g / L, pH=7.0, sterilized at 121°C for 20min), inoculate The amount is 2%, shake culture at 37℃ for 24h, the density of the bacterial solution is 2.65×10 9 cfu / mL to obtain a high-density bacterial suspension.

[0028] 2) Cut 100kg of healthy branches of Nitraria tangutorum into small sections for natural composting, spray 2L of the high-density bacterial suspension prepared in 1) when the internal temperature of the compost reaches 32℃, and continue to compost until the internal temperature of the compost exceeds 43℃ After composting, mixing and cooling, after fermentation in a natural state, it becomes a bio-fertilizer rich in beta-glucanase-producing Bacillus subtilis, that is,...

Embodiment 2

[0038] 1) High-density culture of Bacillus subtilis (CICC 10339) producing beta glucanase, the starting density of Bacillus subtilis (CICC 10339) is 1.70×10 6 Pieces / mL, inoculate into liquid culture medium (glucose 14g / L, beef extract 25g / L, potassium dihydrogen phosphate 2.45g / L, peanut meal powder 10g / L, pH=7.0, sterilized at 121°C for 20min), inoculate The amount is 3%, shake culture at 37℃ for 24h, the density of the bacterial solution is 3.03×10 9 cfu / mL to obtain a high-density bacterial suspension.

[0039] 2) Cut 100kg of healthy branches of Nitraria tangutorum into small sections for natural composting, spray 3L of the high-density bacterial suspension prepared in 1) when the internal temperature of the compost reaches 32℃, and continue to compost until the internal temperature of the compost exceeds 43℃ After composting is completed, stir and cool, and then ferment in a natural state to become a bio-fertilizer rich in Bacillus subtilis that produces beta glucanase.

[00...

Embodiment 3

[0045] 1) High-density culture of Bacillus subtilis (CICC 10339) producing beta glucanase, the starting density of Bacillus subtilis (CICC 10339) is 1.68×10 6 Pieces / mL, inoculate into liquid culture medium (glucose 14g / L, beef extract 25g / L, potassium dihydrogen phosphate 2.45g / L, peanut meal powder 10g / L, pH=7.0, sterilized at 121°C for 20min), inoculate The amount is 3%, shake culture at 37℃ for 24h, the density of the bacterial solution is 2.79×10 9 cfu / mL to obtain a high-density bacterial suspension.

[0046] 2) Cut 100kg of healthy branches of Nitraria tangutorum into small sections for natural composting, spray 3L of the high-density bacterial suspension prepared in 1) when the internal temperature of the compost reaches 32℃, and continue to compost until the internal temperature of the compost exceeds 43℃ After composting is completed, stir and cool, and then ferment in a natural state to become a bio-fertilizer rich in Bacillus subtilis that produces beta glucanase.

[00...

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Abstract

The invention discloses a method for biologically preventing and controlling the stem rot of nitraria tangutorum bobr and promoting the growth of the nitraria tangutorum bobr. The method comprises the steps of preparing a 109-1010cfu / mL high-density bacterial suspension from bacillus subtilis; shearing healthy branches of the nitraria tangutorum bobr into small segments to compost, spraying the high-density bacterial suspension of the bacillus subtilis in the composting process, and fermenting to obtain a biological fertilizer capable of effectively inhibiting and killing pathological fungi of the nitraria tangutorum bobr; and applying the biological fertilizer to biological prevention and control of the stem rot of the nitraria tangutorum bobr and growth promotion of the nitraria tangutorum bobr. According to the method, the bacillus subtilis capable of generating beta-glucanase is a plant probiotic capable of taking disease resisting and growth promoting effects, and if the bacillus subtilis is added into compost, the effects for biologically preventing and controlling pathological fungi of the nitraria tangutorum bobr and promoting the growth of the nitraria tangutorum bobr are remarkably improved. The method is simple and convenient in composting process treatment, free of wastewater discharge, low in investment and energy consumption, easy to operate, capable of easily realizing large-scale industrial production, favorable in economic benefit and wide in application prospect.

Description

Technical field [0001] The invention relates to the field of preparation of biological control fertilizers, in particular to a method for biological control and resistance to Nitraria stalk rot and growth promotion. Background technique [0002] Nitraria sp Terraces and sandy clay have the characteristics of drought resistance, wind erosion resistance, sand burial resistance, salt-alkali resistance, and strong germination ability. They are important constructive plants in arid desert areas. They are the most widely distributed and the largest in the arid desert areas downstream of the river. Vegetation restoration, wind prevention and sand fixation, maintaining the balance of desert ecosystems, and protecting oasis play an important role. Nitraria tangutorum Bor., a traditional Chinese medicine, is the fruit of Nitraria tangutorum Bor., a plant belonging to the genus Nitraria tangutorum Bor. [0003] Nitraria stalk rot is one of the more serious diseases of Nitraria sphaerocarpa....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08A01P3/00A01P21/00C12R1/125
CPCC05F11/08C05G3/00
Inventor 吴渊胡慧雯徐薇薇阮晖梁莹许丽珍吴孔华莫建平史金昕波缪丹俞雨吴盛彬徐腾洋
Owner ZHEJIANG UNIV
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