Bacterial strain mutagenesis cultivation in membrane bioreactor and adenosine fermentation method

A membrane bioreactor, adenosine deaminase technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of gene number and activity decline

Inactive Publication Date: 2015-05-20
LUOYANG DESHENG BIO TECH CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

DAVID A.GLESNE, FRANK R.COLLART, AND ELIEZER HUBERMANL (MOLECULAR AND CELLULAR BIOLOGY, Nov.1991, p.5417-5425) etc. studied inosinate dehydrogenase inhibitors mycophenolic acid and guanosine on inosinic acid Regulation of dehydrogenase gene expression, indicating that treatment of the inosinate dehydrogenase gene with guanosine and mycophenolic acid for several hours resulted in a decrease in gene number and activity

Method used

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  • Bacterial strain mutagenesis cultivation in membrane bioreactor and adenosine fermentation method
  • Bacterial strain mutagenesis cultivation in membrane bioreactor and adenosine fermentation method
  • Bacterial strain mutagenesis cultivation in membrane bioreactor and adenosine fermentation method

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Embodiment Construction

[0010] The strain is added into the solution containing the enzyme inhibitor, and after 3-6 hours of treatment, the membrane experimental device is started for aerobic fermentation. The oxygen flow rate is about 1.6L / min (2L fermentation liquid), and the pH value is adjusted in the range of 6-6.2 with ammonia water during the fermentation process. During the experiment, the ultraviolet absorbance of the membrane permeate was tested, and the fermentation was stopped when the absorbance did not increase.

[0011] 1. Comparison of the experimental rate of hollow fiber ultrafiltration membrane cell cycle fermentation bioreactor fermentation and conventional fermentation

[0012] Under the condition that the fermentation medium composition is shown in Table 1, see Table 2 and Table 3 for the hollow fiber ultrafiltration membrane cell circulation fermentation bio-fermentation bioreactor and conventional fermentation experiments

[0013] Table 1. Fermentation medium composition

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Abstract

The invention describes a method for producing a bacillus subtillis bacterial strain with adenosine having a concentration of 8g / L and uridine having a concentration of 6g / L through once or two-time mutagenesis. The bacillus subtillis bacterial strain is cultured in a high density in a membrane bioreactor by taking ribavirin, methotrexate, allopurine and bupirimate as enzyme inhibitors to perform cooperative mutagenesis of bacillus subtillis. The result shows that the fermentation rate is increased since the membrane bioreactor is utilized to ferment adenosine.

Description

technical field [0001] The invention belongs to the field of microbial fermentation nucleosides and membrane bioreactors Background technique [0002] The current inosine fermentation includes ammonia-producing short bacteria fermentation (inosine yield 31g / L), Bacillus subtilis fermentation adenosine (16g / L), Bacillus subtilis fermentation guanosine (20g / L), Bacillus subtilis fermentation cytidine (19g / L) L), Bacillus subtilis fermentation of uridine (65 g / L) (Michael Petersen and Andreas Kiener; Biocatalysis; Green Chemistry April 1999). Recent literature reports at home and abroad have not seen a value higher than this value. The Bacillus subtilis adenosine fermentation strain should have the following characteristics: inosinate dehydrogenase deficiency, that is, xanthine deficiency; adenosine deaminase deficiency, cutting off the pathway from adenylate to inosinate. Deficiency of adenosine phosphorylase reduces adenosine breakdown; relieves feedback inhibition of adeno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/01C12N1/20C12P19/40C12R1/125
Inventor 姬朝青薛宝玉王育才苏华强刘爱香
Owner LUOYANG DESHENG BIO TECH CO LTD
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