Salt-tolerant cold-resistant ammonia oxidizing bacterium immobilizing method and application thereof

Abstract

The invention relates to a salt-tolerant cold-resistant ammonia oxidizing bacterium immobilizing method and an application thereof. The salt-tolerant cold-resistant ammonia oxidizing bacterium immobilizing method comprises the following steps: firstly screening out high-efficiency ammonia oxidizing bacteria, carrying out salt-tolerant and cold-resistant domestication on the high-efficiency ammonia oxidizing bacteria to obtain domesticated Ochrobactrum.sp and Aquamicrobium.sp, then putting prepared biological carbon balls into a salt-tolerant cold-resistant ammonia oxidizing bacteria-enriched liquid for carrying out biofilm formation on the biological carbon balls, then mixing with salt-tolerant cold-resistant ammonia oxidizing bacterium embedding mixed liquor, and finally putting the biological carbon balls in the mixed liquor into a saturated boric acid solution one by one, putting the biological carbon balls into a refrigerator, crosslinking, and cleaning, so that salt-tolerant cold-resistant ammonia oxidizing bacterium immobilized biological carbon balls are obtained. The salt-tolerant cold-resistant ammonia oxidizing bacterium immobilizing method has the advantages that the biological carbon balls are utilized as a carrier for immobilizing the salt-tolerant cold-resistant ammonia oxidizing bacteria, a carrier material is local and available, a preparation method is simple, industrialization can be easily realized, the salt-tolerant cold-resistant ammonia oxidizing bacterium immobilized biological carbon balls can be applied to purification of low-temperature high-salt ammonia nitrogen polluted water, microbial biomass is high, mechanical strength is high, treatment effect is good, cost is low, recycling is not carried out, and no secondary pollution is produced to the environment.

Description

technical field [0001] The invention belongs to water pollution control technology, and specifically relates to a method and application of immobilization of salt-tolerant and cold-ammonia-oxidation-resistant bacteria, and relates to the domestication and screening of salt-tolerant and cold-ammonia oxidation-resistant bacteria, as well as its immobilization method and application. Background technique [0002] Excessive ammonia nitrogen is one of the main factors of water eutrophication and the main indicator of water pollution control. At present, the removal methods for ammonia nitrogen at home and abroad mainly include physical methods, chemical methods and biological methods. Physical methods can only realize the displacement of pollutants in space, but cannot achieve complete removal; chemical methods are fast and effective, but due to the introduction of chemical agents, improper treatment will cause secondary pollution to water bodies; biological methods use microbial...

AI Technical Summary

Problems solved by technology

The usual high-efficiency bacterial agents obtained through cultivation and acclimation are added to a fixed-volume reactor. Although a better degradation effect can be obtained by changing the conditions in the reactor, they are applied to natural rivers with on-site flow patterns. The flow of the bacterial agent is dispersed in the water body, and it is difficult to ensure sufficient residence time and sufficient microbial concentration, so a good removal effect cannot be achieved

In addition, the Liaohe Wetland in Northeast China is an important oil mining area. Oil leakage caused by oil extraction, transportation and storage has a toxic effect on microorganisms and greatly restricts the degradation of ammonia nitrogen.

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