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SCML4 gene sequence and expression change detection and application of SCML4 gene sequence in coronary heart disease prediction

A coronary heart disease and gene technology, applied in the detection of SCML4 gene sequence and expression changes and its application in the prediction of coronary heart disease, can solve the problems of lack of effective methods for susceptibility genes and insufficient explanation of the genetic basis of coronary heart disease, etc., to achieve The effect of reducing morbidity

Active Publication Date: 2015-05-27
田小利 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On the other hand, only 6 of the discovered loci are based on the research findings of the Chinese Han population, which is far from enough to explain the genetic basis of coronary heart disease in the Chinese Han population
In addition, most of the existing studies only focus on the related loci, and there is no effective method for identifying the susceptibility genes referred to by the related loci.

Method used

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  • SCML4 gene sequence and expression change detection and application of SCML4 gene sequence in coronary heart disease prediction
  • SCML4 gene sequence and expression change detection and application of SCML4 gene sequence in coronary heart disease prediction
  • SCML4 gene sequence and expression change detection and application of SCML4 gene sequence in coronary heart disease prediction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Example 1: Genome-wide screening of coronary heart disease susceptibility loci:

[0090] Experimental population

[0091] In this embodiment, the samples and data of patients with coronary heart disease, that is, the coronary heart disease case group (positive coronary angiography) came from the General Hospital of the Chinese People's Liberation Army, and the samples and data of the control group came from the Beijing Physical Examination Center. All samples from the population participating in the experiment were notified in writing, and informed consent was signed by themselves or their family members. The experimental operation followed the ethical principles stipulated in the "Declaration of Helsinki" and was reviewed and approved by the Peking University Ethics Committee.

[0092] Among them, the selection criteria for samples from the coronary heart disease case group meet one of the following three conditions:

[0093] (1) Previous medical history of myocardia...

Embodiment 2

[0405] Example 2 Multi-center verification of rs9486729 site

[0406] In order to further verify the rs9486729 single nucleotide polymorphism site in Example 1, this example will carry out multi-center verification on the above site. The method used for verification is still the combination of LCR and direct sequencing. The specific operation is as follows:

[0407] research population

[0408] The samples and data of patients with coronary heart disease (positive coronary angiography) used in the experiment came from the Affiliated Hospital of Harbin Medical University, China-Japan Friendship Hospital, Fuwai Hospital, and the First Affiliated Hospital of Zhengzhou University. The control samples and data came from the physical examination centers in Northeast and North regions that matched the regions of the cases; the case samples and data verified by the southern population came from the First People's Hospital of Jiangsu Province, the First People's Hospital of Ningbo Ci...

Embodiment 3

[0422] Example 3 Identification of susceptibility genes

[0423] Unlike many SNP sites found in GWAS located in gene deserts, the rs9486729 site identified in this application is located in the SCML4 promoter region, so the inventors hypothesized that the SCML4 gene may be a susceptibility gene for coronary heart disease. And verify it through this embodiment.

[0424] research population

[0425] The population used for the eQTL analysis of SCML4 gene came from the Beijing Physical Examination Center. All samples from the population participating in the experiment were notified in writing, and informed consent was signed by themselves or their family members. The experimental operation followed the ethical principles stipulated in the "Declaration of Helsinki" and was reviewed and approved by the Peking University Ethics Committee.

[0426] cell culture

[0427] Human embryonic kidney cell line HEK293A was preserved by this experiment; primary human umbilical vein endothe...

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Abstract

The invention relates to the technical field of biology. On the one hand, the invention relates to a single nucleotide polymorphism (SNP) site of a coronary heart disease susceptibility gene SCML4, and a corresponding composition and a kit for detecting the SNP site, and particularly relates to an application of a nucleic acid affinity ligand of the SNP site in preparation of the composition for detecting, screening or predicting the coronary heart disease susceptibility of Chinese han population; and on the other hand, the invention relates to a corresponding composition and kit for detecting the SCML4 gene, and further relates to an application of the nucleic acid affinity ligand of the SCML4 gene in preparation of the composition for detecting, screening or predicting the coronary heart disease susceptibility of Chinese han population. A new further scientific basis is provided for research and development of specific coronary heart disease treatment; and a new clue is provided for discovery of a new molecular mechanism of the coronary heart disease, development of new drug targets and individual-based treatment of the coronary heart disease.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a single nucleotide polymorphism (single nucleotide polymorphism, SNP) site on the SCML4 gene associated with susceptibility to coronary heart disease, and a corresponding combination for detecting the SNP site The invention relates to a substance and a kit, in particular to the application of the nucleic acid affinity ligand of the SNP site in the preparation of a composition for detecting, screening or predicting the susceptibility of coronary heart disease in Han population. Background technique [0002] Coronary heart disease, also known as coronary heart disease, is an ischemic heart disease caused by coronary artery wall atherosclerosis with morphological changes of coronary artery stenosis or even occlusion. Coronary heart disease is extremely harmful. According to the data of the World Health Organization, ischemic heart disease, mainly coronary heart disease, is the number o...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 田小利李扬
Owner 田小利
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